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RNA Catalysis in Model Protocell Vesicles

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Contribution from the Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.
Cite this: J. Am. Chem. Soc. 2005, 127, 38, 13213–13219
Publication Date (Web):August 31, 2005
https://doi.org/10.1021/ja051784p

Copyright © 2005 American Chemical Society. This publication is licensed under these Terms of Use.

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Abstract

We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation of self-replicating nucleic acids in self-replicating membrane vesicles. Here, we address the issue of the compatibility of these two replicating systems. Fatty acids form vesicles that are able to grow and divide, but vesicles composed solely of fatty acids are incompatible with the folding and activity of most ribozymes, because low concentrations of divalent cations (e.g., Mg2+) cause fatty acids to precipitate. Furthermore, vesicles that grow and divide must be permeable to the cations and substrates required for internal metabolism. We used a mixture of myristoleic acid and its glycerol monoester to construct vesicles that were Mg2+-tolerant and found that Mg2+ cations can permeate the membrane and equilibrate within a few minutes. In vesicles encapsulating a hammerhead ribozyme, the addition of external Mg2+ led to the activation and self-cleavage of the ribozyme molecules. Vesicles composed of these amphiphiles grew spontaneously through osmotically driven competition between vesicles, and further modification of the membrane composition allowed growth following mixed micelle addition. Our results show that membranes made from simple amphiphiles can form vesicles that are stable enough to retain encapsulated RNAs in the presence of divalent cations, yet dynamic enough to grow spontaneously and allow the passage of Mg2+ and mononucleotides without specific macromolecular transporters. This combination of stability and dynamics is critical for building model protocells in the laboratory and may have been important for early cellular evolution.

Introduction

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The ability to evolve through the combined effects of inherited variation and natural selection is the unifying principle of biology. The most elementary cellular system capable of Darwinian evolution requires two components:  a self-replicating informational molecule, such as an RNA polymerase ribozyme (“replicase”), and a mechanism for spatial localization such as compartmentalization. Without compartmentalization or some other mechanism for restricting the diffusion of the genetic material, a superior mutant replicase would act to propagate unrelated sequences more efficiently, while having no effect on its own fitness. The physical proximity of related sequences, which can be readily maintained by the random segregation of sequences within replicating compartments, is therefore necessary for the evolution of improved replicase activity through natural selection. (1-6) Membranes provide the major mechanism for compartmentalization in modern biology and also mediate the chemical fluxes between the cell and its environment.
To construct the simplest possible protocell, the membrane boundary should be capable of growth and division without complex biochemical machinery and should allow the entry of ions and small substrates by passive diffusion. To have potential prebiotic relevance, the membrane should also be formed from chemicals that can be synthesized abiotically. Fatty acids are simple amphiphiles that form bilayer membrane vesicles when the solution pH is near the apparent pKa of the membrane-incorporated fatty acid. (7, 8) They can be synthesized under a variety of conditions, (9, 10) including simulated hydrothermal vent chemistry, (12) and have been detected in carbonaceous chondrite meteorites. (13, 14) Clay surfaces that catalyze RNA polymerization also promote the self-assembly of fatty acid micelles into vesicles, suggesting a route for enhancing RNA encapsulation by colocalizing vesicles and RNA. (15, 16)
In addition to their chemical simplicity, fatty acid membranes have dynamic properties that are not exhibited by phospholipid membranes. For example, fatty acid vesicles show autocatalytic growth, (17, 18) and they can undergo repeated cycles of growth and division in the laboratory. (16) These vesicles can also exhibit a primitive form of competition for limited resources, as vesicles encapsulating a high concentration of RNA grow larger at the expense of empty vesicles; this is referred to below as the exchange pathway for growth. (19) Under certain conditions, growth can drive the formation of a transmembrane pH gradient, thereby storing energy for several hours. (20) These protocellular behaviors are facilitated by the fast time scales of monomer−micelle−vesicle transitions and the relatively small energetic barriers between these phases, (21) as compared to phospholipid membranes, which do not share these dynamic properties.
The major disadvantage of using fatty acid membranes to build protocells is their instability under the ionic conditions necessary for catalytic RNA activity. For example, decanoic acid vesicles aggregate at moderate concentrations of monovalent ions (∼0.2 M NaCl), and divalent cations cause aggregation and precipitation even at quite low concentrations (<1.5 mM MgCl2, or 1:65 ratio of cation to lipid). (22) This is an important problem because divalent cations are often required for the formation of tertiary contacts in RNA folding and also commonly participate directly in the catalytic mechanism. (23, 24) Consequently, the activity of many ribozymes, such as the hammerhead ribozyme, is highly dependent on divalent cation concentration. (25, 26) For a protocell to use RNA-based biochemistry, vesicle membranes must be stable under conditions enabling ribozyme activity. We therefore sought to encapsulate catalytically active RNA in simple vesicles that were stable and capable of growth.
The addition of fatty acid glycerol esters stabilizes fatty acid vesicles in the presence of ionic solutes. (22, 27) Although a variety of cations can be utilized by ribozymes, we focused on Mg2+ because of its common use by ribozymes and its abundance on the earth, particularly in seawater. We characterized the growth properties, Mg2+ tolerance, and Mg2+ permeability of such mixed composition vesicles. By varying the composition of mixed vesicles, we were able to increase the tolerance of vesicles to Mg2+ and to demonstrate that Mg2+ could rapidly enter vesicles by passive diffusion. Remarkably, the presence of Mg2+ increased the permeability of the membrane to small, negatively charged solutes such as nucleotides. On the other hand, vesicle growth by the exchange pathway was slowed, and vesicle growth following micelle addition was only possible after further modification of the membrane composition. Finally, we encapsulated a hammerhead ribozyme in mixed vesicles and showed that the ribozyme is active under conditions consistent with vesicle stability and growth.

Materials and Methods

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Materials. Myristoleic acid (C14:1) and glycerol 1-monomyristoleate (i.e., monomyristolein, the glycerol ester of myristoleic acid) were purchased from NuChek Prep (Elysian, MN). Pyrene was from Fluka (Buchs SG, Switzerland); N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (NBD-PE), Lissamine rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Rh-DHPE), calcein, and mag-fura-2 (tetrapotassium salt) were from Molecular Probes (Eugene, OR). GlycoBlue was purchased from Ambion, Inc. (Austin, TX), and RNasin was from Promega (Madison, WI). RNA labeled with fluorescein (5‘-6-FAM-AAAAAAAAAA-3‘) was synthesized at the W. M. Keck Facility at Yale University. All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
Preparation of Micelles and Vesicles. Micelles were prepared by dissolving neat myristoleic acid oil in 1 equiv of NaOH solution (80 mM), as described. (16) Vesicles 100 nm in diameter were prepared by resuspending 1 equiv of the oil in 0.2 M bicine buffer, pH 8.5, to which 0.5 equiv of NaOH had been added (resulting in a final pH of 8.5). Resuspension was followed by several freeze−thaw cycles and extrusion to 100 nm diameter using the Mini-Extruder system (Avanti Polar Lipids, Inc.), as described. (20) Mixed composition micelles and vesicles were prepared by mixing the appropriate neat oils before resuspension. Mixed micelles were used within 10 min of preparation. Vesicles labeled by the FRET dyes (NBD-PE and Rh-DHPE) were prepared by mixing the dyes with fatty acid/ester in methanol, and then removing the solvent by rotary evaporation before resuspension. (19)
Encapsulation of RNA or water-soluble fluorescent dyes was achieved by mixing the solute with the resuspension buffer before adding the oil. Vesicles were purified from unencapsulated solutes on a gravity-flow size exclusion column (Sepharose 4B). The fluorescent dyes (≤1 kD) and RNA (19 kD) used in these experiments elute at the low molecular weight range of the resin (30 kD), while vesicles (100 nm in diameter) elute with the void volume. Purification and dilution buffers contained a concentration of amphiphiles approximately equal to the critical aggregate concentration, to avoid disintegration of the vesicles.
Vesicle Growth. Vesicles labeled with FRET dyes were diluted to ∼3 mM amphiphile and added to a cuvette. Growth from addition of micelles was initiated by adding micelles from an 80 mM stock solution, with mixing by pipetting for ∼5 s. FRET was measured using the fluorescence ratio between donor (λem 530 nm) and acceptor (λem 586 nm) dyes, with excitation at 430 nm, as described. (16) To convert FRET signal into relative surface area, a standard curve was prepared for each vesicle composition (Figures S1, S2). Growth by intermembrane transfer of amphiphiles from empty vesicles to vesicles encapsulating tRNA was performed as described previously, (19) with the addition of 3.2 mM MgCl2 to the reaction buffer containing 2 mM amphiphile. The concentration of free Mg2+ in this solution was 2 mM, as measured by mag-fura-2 fluorescence.
Dye Leakage Assay. Extruded vesicles encapsulating calcein or 3H-UMP were added to a small volume of a stock solution of MgCl2 (100 mM) to achieve the desired concentration of Mg2+. To determine the extent of dye leakage, vesicles were separated from free dye by size-exclusion chromatography as described above. The integrated fluorescence intensity of each peak was determined by summing the fluorescence intensity of the fractions, as measured on a SpectraMax Gemini EM (Molecular Devices). For vesicles of this size (∼100 nm), the concentration of vesicles was low enough (<10 mM lipid) to avoid effects on the fluorescence from turbidity. Levels of free dye >6% were reliably detectable as leakage. Radioactivity was measured on a Beckman LS 6500 scintillation counter. For time courses, the percent of free dye in the initial time point was used as the background and subtracted from subsequent time points. Calcein fluorescence was measured at λex 415 nm, λem 600 nm; when appropriate, values were corrected for self-quenching using a standard curve. (16) Initial rates of leakage were determined by fitting a straight line to the data for leakage over time.
Mg2+ Assays. Free Mg2+ concentration was measured using the fluorescent probe mag-fura-2. Mg2+ concentration was related to the ratio of the fluorescence intensity at λex 340 nm and λex 370 nm (at constant λem 500 nm) through a standard curve prepared with varying concentrations of MgCl2 in 0.2 M bicine, pH 8.5. To determine Mg2+ permeability, vesicles containing encapsulated mag-fura-2 were diluted to 4 mM amphiphile and mixed by stopped-flow with 1/25 volume of 50 mM MgCl2 (final [Mg2+] = 2 mM), as described. (21) Because the counter-transport of Na+, required for charge neutrality, is fast relative to the transport of Mg2+, (20) the Mg2+ permeability coefficient was calculated by P = k*V/A, where k is the first-order rate constant, and V and A are the volume and surface area of the vesicle, respectively.
Characterization of Aggregates and Vesicles. Hydrodynamic diameter was estimated by dynamic light scattering, as described. (16) The pyrene excimer/monomer ratio was determined from the ratio of fluorescence intensity at λem 374 nm (monomer) and λem 470 nm (excimer), as described. (21) Amphiphile samples were prepared with 0.5 mol % pyrene.
Hammerhead Ribozyme RNA. The transcription template for the hammerhead ribozyme N15min7 (5‘- GGGACGCAGTTTCGCTTAGCTCATCAGAGTAAATTCCTTTCGGAATTTAC TGACTGCGT-CCCTATAGTGAGTCGTATTACAGATC-3‘) was PCR amplified using the primers (5‘-GATCTGTAATACGACTCAC-3‘ and 5‘-GGGACGCAGTTTCGCTTAG-3‘) for 20 cycles. The PCR product was purified using agarose gel electrophoresis, ethanol precipitated, dissolved, and stored in 10 mM Tris-Cl, pH 8.5.
RNA was transcribed from double-stranded N15min7 template by T7 RNA polymerase in a solution containing 0.5 mM NTPs, ∼20 μCi α-32P-UTP, 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 2 mM spermidine, 10 mM DTT, and 0.2 U/μL RNasin. The reaction also included 25 μM of two oligonucleotides complementary to the ribozyme sequence, to block ribozyme self-cleavage during transcription (5‘-TCGCTTAGCTCATCAG-3‘ and 5‘-GGAATTTACTGACTGC-3‘). (28) Transcribed RNA was purified by denaturing PAGE, ethanol precipitated, dissolved in water, and stored at −80 °C.
Hammerhead Ribozyme Assay. Cleavage reactions were begun by the addition of MgCl2. The reaction was stopped at each time point by removing an aliquot and adding 1.1−2.3 volumes of 10 mM Tris-Cl, pH 7.5, containing 3−14 mM EDTA. For encapsulated reactions, the amphiphile concentration was ∼15 mM and the RNA concentration was 40−300 nM (less than one molecule per vesicle on average). Vesicles were disrupted by the addition of 0.3% Triton X-100, and the RNA was precipitated in 0.3 M sodium acetate, pH 7, 73% ethanol, with 10 μg of GlycoBlue. Pellets were resuspended in formamide loading buffer and resolved by denaturing PAGE. Cleavage products were quantitated by phosphorimage analysis of the dried gel. The extent of cleavage observed before addition of MgCl2 (t = 0) was subtracted from subsequent time points.
Fluorescence Microscopy. Vesicles were prepared by mixing MA:GMM:dodecane (2:1:0.3) oil with buffer consisting of 0.2 M bicine, pH 8.5, 3 mM MgCl2, and 40 nM N15min7 (final amphiphile concentration = 20 mM). Vesicle suspensions were allowed to equilibrate for at least 1 h before staining with 10 mM Rhodamine 6G (λmax,ex = 528 nm; λmax,em = 551 nm) for microscopy. Conventional epifluorescence images were collected using a Zeiss Axioplan 2 microscope (100× objective lens; rhodamine filter). Confocal images were collected using a Leica TCS SP confocal laser scanning microscope equipped with Ar, Ar−Kr, and He−Ne lasers (100× objective lens; 514 nm laser line).

Results and Discussion

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Effect of Mg2+ on the Stability and Permeability of Simple Vesicles. We assessed the stability of vesicles composed of myristoleic acid (MA) and glycerol monomyristoleate (GMM) in the presence of Mg2+ by monitoring the retention of encapsulated dye and macroscopic turbidity changes. Pure MA vesicles showed low Mg2+ tolerance, but vesicles of mixed composition, MA:GMM (2:1), showed increased stability in the presence of Mg2+, as previously reported on the basis of light microscopy. (22) An intermediate composition of MA:GMM (4:1) showed intermediate stability (Table 1). Higher proportions of GMM led to the appearance of oil droplets mixed with vesicles.

Table 1. MgCl2 Tolerance of Simple Vesicles

MA:GMM  ratio [MgCl2]  tolerated,  assayed by  dye leakagea  (mM) [MgCl2] at  turbidity  changeb  (mM)
1:0 0.5 1
4:1 2 3
2:1 4 6

a Dye leakage was assessed <1 h after addition of MgCl2, and onset of leakage defined the maximum [MgCl2] tolerated.b Turbidity change was visible as a sudden increase in the opacity of the solution.

To determine whether vesicles exposed to relatively high concentrations of MgCl2 are only transiently destabilized by the sudden addition of MgCl2, we followed the leakage of encapsulated calcein over time. Dye leakage continued to increase over the course of at least 1 day (Figure 1A,B), indicating that the vesicles remained permeabilized throughout the experiment. The initial rate of dye leakage from MA:GMM (2:1) vesicles increased by a factor of 27 in the presence of 4 mM Mg2+. To determine whether this enhanced permeability was due to large-scale membrane destabilization by Mg2+, we encapsulated a fluorescently labeled RNA decamer in vesicles exposed to 4 mM Mg2+. We expected that large-scale membrane defects would cause RNA leakage at a rate similar to small molecule leakage, but no RNA leakage was detected after 19 h (Figure 1C). 3H-UMP showed a 4-fold permeability enhancement in the presence of 4 mM Mg2+ (Figure 1D). Thus, exposure to Mg2+ increased the permeability of an RNA mononucleotide but not of RNA itself, perhaps by neutralizing the negative charge and/or stabilizing the interaction between the solute and small-scale transient defects in the membrane. (29) Even so, more than 95% of encapsulated calcein was retained in MA:GMM (2:1) vesicles for at least 3 h. This is much longer than the hammerhead ribozyme cleavage reactions described below, which were completed within a few minutes.

Figure 1

Figure 1. Leakage from MA:GMM vesicles (2:1) in 0.2 M bicine, pH 8.5, 4 mM MgCl2. (A) Vesicles were initially purified away from unencapsulated calcein, and dye leakage was measured over time with (blue) or without (black) the addition of 4 mM MgCl2. (B) Calcein leakage assayed by size-exclusion chromatography after 22 h in the presence of 4 mM MgCl2. (C) RNA leakage assayed by size-exclusion chromatography after 19 h, with (red) or without (black) the addition of 4 mM MgCl2. (D) Vesicles encapsulating 0.1 mM 5‘-UMP and a trace amount of 3H-UMP were initially purified, and then leakage of 3H-UMP was measured over time, with (red) or without (black) the addition of 4 mM MgCl2.

Because we wished to eventually measure the activity of encapsulated RNA, we also determined the permeability of Mg2+ through the vesicle membrane. We encapsulated mag-fura-2, (30) a magnesium-sensitive fluorescent dye (Figure 2A), in MA:GMM vesicles (2:1) and added MgCl2 using a stopped-flow device. The internal concentration of Mg2+ equilibrated within several seconds (permeability coefficient = 2 × 10-7 cm/s) to 1.4 mM, a concentration lower than the added concentration (2 mM; Figure 2B). Because MA is partially negatively charged at pH 8.5, it may associate with Mg2+, thereby reducing the amount of free Mg2+ detected by the mag-fura-2 dye. Using size-exclusion chromatography, we verified that <5% of encapsulated mag-fura-2 leaked from vesicles after 30 min of exposure to 2 mM Mg2+ (Figure 2C), consistent with the results obtained for calcein leakage. When we mixed phospholipid (POPC) vesicles with MgCl2, we saw no detectable change in the internal Mg2+ concentration over several hours (Figure 2D), demonstrating that equilibration of both Mg2+ and mag-fura-2 across POPC membranes is quite slow.

Figure 2

Figure 2. Mg2+ permeability in MA:GMM vesicles (2:1). (A) Standard curve of mag-fura-2 fluorescence ratio as a function of [Mg2+] in 0.2 M bicine, pH 8.5. The solid line is a linear regression fit. MgCl2 was added to a final concentration of 2 mM, by stopped-flow mixing, to MA:GMM (2:1) vesicles (B) or POPC vesicles (D) encapsulating mag-fura-2. The internal concentration of Mg2+ at each time point was calculated from the standard curve. The solid line (B) indicates a curve fit to a single-exponential equation, where k = 0.11 s-1. (C) Size-exclusion chromatography shows that no mag-fura-2 dye has leaked out of the MA:GMM vesicles after equilibration of Mg2+.

Growth of Mg2+-Tolerant Vesicles. Although the addition of GMM to MA vesicles increased their tolerance for Mg2+, we suspected that the increase in the stability of the vesicles might come at the expense of dampened dynamic behaviors, such as growth, that rely on the rapid insertion and dissociation of molecules from the membrane. Growth of the surface area of vesicles can be monitored using nonexchanging FRET dyes, whose fluorescence depends on the surface density of the dyes. (16, 31, 32) We have previously studied the growth of osmotically swollen vesicles due to intermembrane transfer of amphiphiles from empty vesicles. While transfer was slower in MA:GMM (2:1) membranes, the total amount of transfer was comparable in MA:GMM (2:1) and pure fatty acid membranes. (19) To determine whether Mg2+ affected the intermembrane transfer of amphiphiles, we measured the transfer between MA:GMM (2:1) vesicles (2 mM amphiphile) in the presence of 3.2 mM MgCl2 (2 mM free Mg2+). The rate (k = 0.05 min-1) and amount of transfer (28%) were not substantially affected by the presence of Mg2+.
Another way to achieve vesicle growth is by the addition of micelles, prepared at high pH, to preformed vesicles buffered at pH 8.5. When 0.5 equiv of pure fatty acid in micelle form is rapidly added to preformed vesicles, the surface area of preformed vesicles grows by ∼50%, indicating a very high yield of incorporation. (16, 21) In contrast, 4:1 MA:GMM vesicles showed only ∼35% growth upon addition of 0.5 equiv of micelles of the same composition. Dynamic light scattering (DLS) indicated that MA:GMM (4:1) micelle solutions contained particles ∼30 nm in diameter, much larger than pure fatty acid micelles (diameter = 3 nm). (20) Aggregation can also be detected by the excimer/monomer ratio of the hydrophobic dye pyrene when present at low molar ratio (∼1:200). When dissolved in small micelles, pyrene is distributed among the separate volumes and exists primarily as a monomer. However, lipid aggregation allows multiple pyrene molecules to share a contiguous volume, forming excimers with a red-shifted fluorescence emission spectrum. (33-35) Consistent with the DLS data, pyrene fluorescence indicated the presence of large aggregates in MA:GMM (4:1) micelles (Figure 3A).

Figure 3

Figure 3. Growth of mixed composition vesicles by micelle incorporation. (A) Pyrene fluorescence shifts from monomer to excimer if aggregates are present. Shown are the spectra of pyrene in pure MA micelles (green), pure MA vesicles (blue), and MA:GMM (4:1) micelles (red). (B) Growth of 2:1:0.3 MA:GMM:dodecane vesicles over time, after addition of 1 equiv of micelles, in 0.2 M bicine, pH 8.5, 1 mM MgCl2. Relative surface area was determined using the FRET assay. The solid line indicates a curve fit to a single-exponential equation, with k = 1.5 min-1. A similar yield was obtained if 1 equiv of micelles was added at once or in up to five separate aliquots.

MA:GMM (2:1) vesicles, which had the greatest magnesium tolerance, showed no growth when micelles of the same composition were added, either rapidly or slowly over 4 h. However, these vesicles were able to grow following the rapid addition of pure fatty acid micelles. Thus, 2:1 MA:GMM micelles (which are also large by the pyrene excimer assay and by DLS) appeared to be unusually stable, perhaps due to the large headgroup of GMM, which may stabilize areas of high curvature in micellar aggregates and which would also provide H-bond donors at high pH. To destabilize these micelles, we added dodecane (9 mol %) to act as a hydrophobic spacer, reducing the preferred curvature and decreasing the surface charge density. The rapid addition of 0.5 equiv of 2:1:0.3 MA:GMM:dodecane micelles to vesicles of the same composition resulted in ∼20% growth, and the rapid addition of 1 equiv of micelles resulted in ∼40% growth (Figure 3B). The rate and total amount of growth were similar in the presence or absence of 1 mM free Mg2+.
Ribozyme Activity in Simple Vesicles. We determined the activity of a self-cleaving hammerhead ribozyme encapsulated in MA:GMM:dodecane vesicles. The hammerhead ribozyme is widespread in nature and also arises during in vitro selection for self-cleaving RNAs. (28, 36-38) This ribozyme can catalyze both cleavage and ligation of RNAs, (39, 40) activities that may be important for simple RNA-based replicating systems. Furthermore, because of its small size, the hammerhead ribozyme may function robustly under error-prone replication conditions. (41) N15min7 is an artificially evolved 62 nucleotide hammerhead ribozyme, which is cleaved into two fragments (11 and 51 nucleotides) in the presence of Mg2+. (42)
N15min7 was encapsulated in 2:1:0.3 MA:GMM:dodecane vesicles during amphiphile resuspension. Multiple freeze−thaw cycles, used to equilibrate vesicle formation, did not affect self-cleavage activity. The self-cleavage reaction was initiated by adding MgCl2 (≤4 mM), and the extent of self-cleavage was monitored as a function of time (Figure 4A,B). We found that ∼60% of the RNA was cleaved whether the ribozyme was encapsulated or free in solution. The observed first-order rate constant of the encapsulated reaction was reduced 4-fold as compared to the unencapsulated reaction at both 1 and 4 mM added MgCl2 (Table 2). As expected, the rate of the encapsulated reaction in 4 mM added MgCl2 was ∼5-fold higher than that in 1 mM added MgCl2.

Figure 4

Figure 4. Self-cleavage activity of the hammerhead ribozyme N15min7. The insets show phosphorimages of the assay gels. The top band corresponds to the uncleaved ribozyme; the bottom band is the cleavage product. (A) Unencapsulated ribozyme activity in 0.2 M bicine, pH 8.5, 4 mM MgCl2. Time points, from left to right:  0 (no MgCl2), 0.2, 0.4, 0.6, 0.8, 1.1 min. The solid line indicates a curve fit to a single-exponential equation, y = a(1 − e-kx), where the extent of cleavage a = 0.66, and the observed rate constant k = 7.9 min-1. (B) Activity of ribozymes encapsulated in 2:1:0.3 MA:GMM:dodecane vesicles (∼15 mM amphiphile), in 0.2 M bicine, pH 8.5, 4 mM MgCl2. Time points, from left to right:  0 (no MgCl2), 0.33, 0.63, 1, 1.3, 1.7, 3, 4.7 min. Curve fit:  a = 0.6, and k = 1.7 min-1. (C) Size-exclusion chromatography of MA:GMM:dodecane vesicles shows that all radiolabeled N15min7 RNA remained encapsulated 15 min after the addition of MgCl2.

Table 2. Hammerhead Cleavage in Vesicles

[MgCl2]  (mM) kunencapsulated  (min-1) kencapsulated  (min-1)
1 1.3 0.4
4 7.9 1.7
This decreased rate of self-cleavage of the encapsulated ribozyme may be partly due to the time scale of Mg2+ permeation into GMM-MA-dodecane vesicles (k ≈ 1 min-1), particularly in 4 mM MgCl2. However, for the encapsulated ribozyme in 1 mM MgCl2, the rate of Mg2+ entry is faster than the observed self-cleavage, indicating that Mg2+ entry was not the rate-limiting step in this case. The decrease in free Mg2+ concentration (Kd ≈ 10 mM amphiphile, considering both the inner and the outer leaflets of the bilayer), as determined by mag-fura-2 fluorescence, accounts for about one-half of the observed decrease in rate. To confirm that the observed self-cleavage activity reflected encapsulated ribozyme, rather than ribozyme adsorbed to the membrane, N15min7 RNA was added to the exterior of vesicles after extrusion but before purification. After purification by size-exclusion chromatography, these preparations contained <3% of the radioactivity of the encapsulated reaction, indicating that only a small amount of RNA was adsorbed to the membrane. We also used size-exclusion chromatography to confirm that the ribozyme still co-purified with vesicles after the self-cleavage reaction (Figure 4C).
We confirmed that the 2:1:0.3 MA:GMM:dodecane mixture formed vesicles in the presence of Mg2+ and RNA by fluorescence microscopy before extrusion (Figure 5). DLS of these vesicles after extrusion indicated an average particle diameter of 65 nm, similar to the size of extruded vesicles made from myristoleic acid alone. (16) Furthermore, self-cleavage of N15min7 was neither catalyzed nor inhibited by the presence of vesicles alone. In the absence of Mg2+, no self-cleavage was detected after 30 min, and the self-cleavage rate of unencapsulated N15min7 upon addition of Mg2+ was not affected by the presence of vesicles.

Figure 5

Figure 5. Fluorescence microscopy of 2:1:0.3 MA:GMM:dodecane vesicles containing hammerhead ribozyme in the presence of 3 mM MgCl2, prior to extrusion. Membranes were stained using Rhodamine 6G for visualization by (A) epifluorescence or (B) laser scanning confocal microscopy. The images shown are from different fields of view.

Hammerhead ribozymes vary greatly in their Mg2+ requirements. The [Mg2+]1/2 (concentration of Mg2+ corresponding to half-maximal activity) of the N15 construct used in our studies is ∼9 mM, (42) and hammerhead ribozymes of biological origin have even lower Mg2+ optima of <1 mM. (43) Other complex natural ribozymes that function under physiological conditions (<1 mM) have evolved low Mg2+ requirements. (44) For example, the [Mg2+]1/2 for folding and catalysis of a number of self-splicing group I introns is ≤4 mM. (45, 46) An RNA polymerase ribozyme with a similarly low Mg2+ requirement could function inside MA:GMM vesicles. However, currently available ribozymes with polymerase or ligase activity have much higher Mg2+ requirements. For example, the class I ligase, discovered by in vitro evolution in the presence of high concentrations of Mg2+, has a [Mg2+]1/2 of 70−100 mM, (47) as does a variant that can catalyze limited RNA polymerization. (48) The discrepancy between the Mg2+ requirement of this ribozyme and the Mg2+ tolerance of the simple vesicles studied here suggests that observing ribozyme polymerase activity inside vesicles that can grow and divide may not be trivial in practice. Further optimization of existing ribozymes, or isolation of new ones, may yield RNA polymerases active at lower Mg2+ concentrations that could be compatible with the vesicles described here. An independent reason for focusing on low to moderate Mg2+ concentrations is that high Mg2+ concentrations promote RNA degradation. (49) Mg2+-catalyzed RNA degradation is in fact thought to limit the extent of polymerization obtainable by the polymerase derived from the class I ligase. (48)

Conclusions

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We have shown that vesicles composed of chemically simple amphiphiles can be stable, grow, and retain contents in the presence of sufficient Mg2+ to activate encapsulated ribozymes. Because these membranes, unlike phospholipid membranes, are quite permeable to Mg2+, encapsulated ribozymes can be activated by the external addition of Mg2+. Other RNA-catalyzed reactions, including RNA replication, may ultimately be possible within similar vesicles, enabling the experimental construction of evolvable protocells. The permeability of these simple membranes to Mg2+ means that the internal Mg2+ concentration of a population of growing and dividing vesicles could be maintained indefinitely, from generation to generation, solely by passive trans-membrane diffusion in the absence of permeases.
Our experiments also illustrate that there are tradeoffs in the search for conditions compatible with both RNA and vesicle replication. While the addition of GMM confers stability on MA vesicles, it also causes a decrease in the yield of growth following micelle addition. This decrease could be partially offset by the addition of another simple component, dodecane. On the other hand, the yield of growth of osmotically swollen vesicles was not affected by the addition of GMM. (19) Indeed, this mode of growth may be more likely to occur in natural situations, because the source of lipid, empty vesicles, is the same physical phase as the growing vesicles, readily permitting their coexistence.
An unexpected effect of Mg2+ was to increase the permeability of small, negatively charged solutes, including uridine monophosphate. This effect would be advantageous to an encapsulated replicating RNA that was polymerizing nucleic acid monomers into longer strands of RNA. The increase in monomer permeability might enhance polymerization, while the polymeric product would continue to be retained.
Many selective pressures are likely to have influenced the evolution of the phospholipid membranes of modern biology, which are much more stable to divalent cations and can maintain pH and ionic gradients for long periods. However, the evolution of such membranes would have required the co-evolution of numerous ion and substrate-specific permeases, as well as the biochemical machinery for membrane growth. Our results show that membranes made from simple amphiphiles can be stable enough to retain RNA, yet dynamic enough to grow and allow the spontaneous entry of Mg2+ and mononucleotides. We are continuing to search for membrane compositions with similar dynamic properties, but greater tolerance to Mg2+ and possibly Ca2+ (another abundant divalent cation on earth), as well as ribozyme polymerases active at lower Mg2+ concentrations, with the goal of observing RNA-catalyzed RNA synthesis within model protocell vesicles.

Supporting Information

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Supporting Information Available

Standard curve for FRET signal prepared using 4:1 MA:GMM vesicles (Figure S1) and 2:1 MA:GMM vesicles (Figure S2). This material is available free of charge via the Internet at http://pubs.acs.org.

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  • Corresponding Author
    • Jack W. Szostak - Contribution from the Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
  • Authors
    • Irene A. Chen - Contribution from the Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
    • Kourosh Salehi-Ashtiani - Contribution from the Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114

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We are grateful to Pierre-Alain Monnard, Martin Hanczyc, Shelly Fujikawa, Sheref Mansy, Justin Ichida, Michael Sacerdote, James Carothers, and Andrej Luptak for valuable discussions. We thank Gary Ruvkun and Frederick Ausubel for the use of their microscopes. J.W.S. is an investigator of the Howard Hughes Medical Institute. I.A.C. was supported by the NIH Medical Scientist Training Program (#T32-GM07753) and an NIH Molecular Biophysics Training Grant (#T32-GM08313). This work was supported in part by a grant from the NASA Exobiology Program (EXB02-0031-0018).

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  • Figure 1

    Figure 1. Leakage from MA:GMM vesicles (2:1) in 0.2 M bicine, pH 8.5, 4 mM MgCl2. (A) Vesicles were initially purified away from unencapsulated calcein, and dye leakage was measured over time with (blue) or without (black) the addition of 4 mM MgCl2. (B) Calcein leakage assayed by size-exclusion chromatography after 22 h in the presence of 4 mM MgCl2. (C) RNA leakage assayed by size-exclusion chromatography after 19 h, with (red) or without (black) the addition of 4 mM MgCl2. (D) Vesicles encapsulating 0.1 mM 5‘-UMP and a trace amount of 3H-UMP were initially purified, and then leakage of 3H-UMP was measured over time, with (red) or without (black) the addition of 4 mM MgCl2.

    Figure 2

    Figure 2. Mg2+ permeability in MA:GMM vesicles (2:1). (A) Standard curve of mag-fura-2 fluorescence ratio as a function of [Mg2+] in 0.2 M bicine, pH 8.5. The solid line is a linear regression fit. MgCl2 was added to a final concentration of 2 mM, by stopped-flow mixing, to MA:GMM (2:1) vesicles (B) or POPC vesicles (D) encapsulating mag-fura-2. The internal concentration of Mg2+ at each time point was calculated from the standard curve. The solid line (B) indicates a curve fit to a single-exponential equation, where k = 0.11 s-1. (C) Size-exclusion chromatography shows that no mag-fura-2 dye has leaked out of the MA:GMM vesicles after equilibration of Mg2+.

    Figure 3

    Figure 3. Growth of mixed composition vesicles by micelle incorporation. (A) Pyrene fluorescence shifts from monomer to excimer if aggregates are present. Shown are the spectra of pyrene in pure MA micelles (green), pure MA vesicles (blue), and MA:GMM (4:1) micelles (red). (B) Growth of 2:1:0.3 MA:GMM:dodecane vesicles over time, after addition of 1 equiv of micelles, in 0.2 M bicine, pH 8.5, 1 mM MgCl2. Relative surface area was determined using the FRET assay. The solid line indicates a curve fit to a single-exponential equation, with k = 1.5 min-1. A similar yield was obtained if 1 equiv of micelles was added at once or in up to five separate aliquots.

    Figure 4

    Figure 4. Self-cleavage activity of the hammerhead ribozyme N15min7. The insets show phosphorimages of the assay gels. The top band corresponds to the uncleaved ribozyme; the bottom band is the cleavage product. (A) Unencapsulated ribozyme activity in 0.2 M bicine, pH 8.5, 4 mM MgCl2. Time points, from left to right:  0 (no MgCl2), 0.2, 0.4, 0.6, 0.8, 1.1 min. The solid line indicates a curve fit to a single-exponential equation, y = a(1 − e-kx), where the extent of cleavage a = 0.66, and the observed rate constant k = 7.9 min-1. (B) Activity of ribozymes encapsulated in 2:1:0.3 MA:GMM:dodecane vesicles (∼15 mM amphiphile), in 0.2 M bicine, pH 8.5, 4 mM MgCl2. Time points, from left to right:  0 (no MgCl2), 0.33, 0.63, 1, 1.3, 1.7, 3, 4.7 min. Curve fit:  a = 0.6, and k = 1.7 min-1. (C) Size-exclusion chromatography of MA:GMM:dodecane vesicles shows that all radiolabeled N15min7 RNA remained encapsulated 15 min after the addition of MgCl2.

    Figure 5

    Figure 5. Fluorescence microscopy of 2:1:0.3 MA:GMM:dodecane vesicles containing hammerhead ribozyme in the presence of 3 mM MgCl2, prior to extrusion. Membranes were stained using Rhodamine 6G for visualization by (A) epifluorescence or (B) laser scanning confocal microscopy. The images shown are from different fields of view.

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    Standard curve for FRET signal prepared using 4:1 MA:GMM vesicles (Figure S1) and 2:1 MA:GMM vesicles (Figure S2). This material is available free of charge via the Internet at http://pubs.acs.org.

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