Abstract
In most experimental conditions, the initial concentrations of a chemical system are at stoichiometric proportions, allowing us to eliminate at least one variable from the mathematical analysis. Under different initial conditions, we need to consider other manifolds defined by stoichiometry and the principle of conservation of mass. Therefore, a given set of initial conditions defines a dynamic manifold and the system, a tall times, has to satisfy a particular relation of its concentrations. To illustrate the relevance of the initial conditions in a dynamic analysis, we consider a chemical system consisting of two first-order self-replicating peptides competing for a common nucleophile in a semi-batch reactor. For the symmetric case, we find different complex oscillations for a given set of parameter values but different initial conditions.
Keywords
- chemical self-replication
- limiting reagent
- coexistence
- autocatalytic self-replication
- chemical systems
1. Introduction
Chemical self-replication is how an individual molecule can duplicate itself. In a first-order process, a product molecule directs in own synthesis by facilitating the binding of two or more component molecules to form a new product molecule. The product molecule acts as an auto-catalytic template to position the components for a ligation reaction. There are two critical steps in chemical self-replication. First, the product molecules must bind available components to facilitate the ligation and formation of the product molecules. Second, once this ligation is completed, the product-template complex (duplex) must readily dissociate so that the newly formed product molecule may join the other product molecules. In an efficient self-replicating system, as soon as the new product molecule is formed after ligation, it should readily dissociate from the template molecule and begin to act as a template by binding component molecules. Since product molecules can participate in multiple replication cycles as a template, the product concentration’s growth rate is directly proportional to its concentration. This relationship characterizes autocatalysis, and it yields an exponential growth rate for the concentration of the product molecule. This process of sustained exponential growth is known as autocatalytic self-replication.
In reality, however, it is not easy to achieve the delicate balance between a strong binding of the component molecules to facilitate the formation of a new product molecule and an easy dissociation of the duplex, which is required for autocatalytic self-replication. The most significant challenge is the difficulty of dissociating the product-template intermediate to yield a new product molecule and the original template. When product-template molecules remain together, the number of template molecules is reduced, and thus the growth rate is less than exponential.
Over the past 25 years, the interest in understanding chemical self-replication has grown. Researchers have developed several experimental systems in aqueous solutions using peptides [1, 2, 3, 4, 5, 6, 7, 8, 9, 10], oligonucleotides [11, 12, 13, 14, 15], modified ribozymes [16, 17, 18], and DNA [19, 20, 21, 22, 23, 24]. In particular, we have considered Joyce’s [16, 17, 18], Ashzkenazy’s [25, 26, 27, 28, 29, 30, 31, 32, 33, 34], and Rebek’s [35, 36, 37, 38, 39, 40] experimental systems and have proposed simple model [41] to analyze their experiments.
In most reported cases, researchers start with an initial set of concentrations and monitor over time the concentrations of the reactants, product, and, on some occasions, the intermediate. Under these so-called batch conditions, researchers have found exponential growth and, therefore, self-replication. Using Joyce’s experiments, we have proposed a minimal Templator Mode [41] and determined parameter values for our model, and we have extended our analyses to open conditions to characterize probable dynamic behaviors [41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51].
However, most of our early work has only emphasized auto-catalysis mediated by a single template, and, lately, we have considered a generalization of the first model to include the so-called parabolic growth and the association of multiple product/template molecules to form an active auto-catalytic template multimer [45, 46]. We have also considered cross-catalysis, where a system of four component molecules and two templates can cross-replicate [47]. In other words, one template catalyzes the formation of the other template and vis versa.
Another critical aspect of chemical self-replication is its implications for understanding life’s origins [52, 53, 54, 55]. Although chemical self-replication is necessary to develop models of the origins of life, competition between chemical systems must also be included in the discussion. To consider competition in our analysis of chemical self-replication, we study a two-template system competing for one common reagent. In the next section, we discuss an extension of our previous work to include the three different reactants and two self-replicating templates. Section 3 presents and discusses our dynamic analysis in cases where the templates are similar, but one is a better replicator than the other. Finally, section 4 summarizes the dynamic behavior of a simple competing system of self-replicating templates.
2. General model
In previous work [41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51], we have considered Rebek’s and Joyce’s self-replicating systems and modeled ideal self-replication using a self-complementary template mechanism. For this experimental design, we used a reasonable chemical model consistent with the laboratory work on self-replication. In particular, we consider a simple self-replicating mechanism characterized by a cubic nonlinearity, and in general, chemical self-replication can be represented schematically by the following mechanistic steps
where
While considering first-order self-replication, we can couple the autocatalytic process with an enzymatic formation of a regulatory product, Q,
where the rate shows saturation at high concentrations of
Following Joyce’s ribozyme systems and Ashkenazy’s peptide systems, where the components are RNA strands or electrophilic or nucleophilic peptide fragments, we build a competitive Templator model by first considering the uncatalyzed formation of the template
In Eqs. (4) and (5),
Here,
As in most of our previous work, we have considered a simple constant volume ideal open conditions that assume a continuous inflow of reactants to the system. Therefore, to prevent the chemical system from reaching equilibrium, we pump A, B, and C into the system from pools
Finally, we must incorporate the removal of each template from the system through an enzymatic reaction that converts them to another compound,
where
Based on Eqs. (4) and (5), we can consider a competitive two-template system with the following ODEs:
For details on dimensionless systems, see references [41, 43].
However, before analyzing the dynamic behavior of these five ODEs, we must understand the behavior of the chemical system in the absence of one of the competing templates, the role of the external fluxes, and the initial conditions on the dynamics. Therefore, if we ignore the competing template,
Since the overall reaction shows that A and B are in a one-to-one relation, we next employ the following simple definitions:
Where X(t) stands for the total concentration of A and B, and Y(t) represents the difference in concentration between A and B, both at a given time t. From Eqs. (21) and (22), we determine the new ODEs,
where
Notice that in self-replicating chemical systems, the uncatalyzed process occurs with a very low probability, so
In previous work, we have analyzed at length the case of no-limiting reagent,
or
In other words, the difference in initial concentrations introduces a mass constraint on the dynamic behavior of the concentrations.
Regardless of initial conditions, we find a linear relationship (with a slope
In Figure 1, we consider the following dimensionless parameter values:
As in the three-variable system, in competitive scenarios, stoichiometric pumping is necessary for the existence of a steady state. It is straightforward to prove that a steady state exists if the pumping pools satisfy the stoichiometric condition
3. Dynamic characterization of the symmetric case
In this section, we examine the symmetric case in which both templates exhibit the same replicative efficiency for their catalyzed and uncatalyzed formation. Although we may think the symmetric case is not that interesting, an initial limiting reagent condition can give us interesting dynamic behaviors. In Figure 2, we plot the time Series [56] for
For example, in Figure 4, we hold A(0) at 40 and C(0) at 20 while increasing B(0) to 45 (Y = −25), and, in this case, we find period two, (P2), out of phase oscillations with larger amplitude that in Figure 3. At this point, we have changed
Upon further increase of the initial concentration of B to 25 while holding A(0) and C(0) at the same values (Y = −5), the chemical system returns to simple alternating oscillations, with template R exhibiting greater amplitude (Figure 3). Inspection of the case in which A(0) = B(0) = C(0) = 20 (Y = −20) indicates that the system exhibits the same behavior, though the amplitude values of the templates are approximately the same (Figure 4). We again see this simple oscillatory behavior when Y = −25 (Figure 5), but when Y = −26, the system transitions to chaotic behavior (Figure 6). We find another transition in behavior in the competitive model when Y is equal to −37 at higher values of B(0) as it begins to exhibit complex rather than chaotic oscillations (Figure 7). At more extreme values of B(0) (Y = −50), we continue to see complex behavior in this system (Figure 8).
4. Discussion
We can emphasize the synchronized and unsynchronized oscillations in figures for the symmetric case (2,3). The synchronized oscillations have a smaller amplitude than the case of unsynchronized oscillations. But, in general, the oscillations tend to be unsynchronized, as shown in Figures 4–8, and, in the cases of the plots in phase space (P, R), where the symmetry is easy to observe. In the previous section, we have shown simple P1 and P2 oscillations and complex oscillations. The symmetric case serves as a benchmark as we continue our analysis.
At first glance, we may inquire why the initial conditions influence the observed attractor. We emphasize that one needs to satisfy the condition
where
The restriction due to the mass conservation expressed in the reaction’s stoichiometry is not particular to the assumed system’s conditions. The mass constraint manifests through the initial concentrations for our ideal open system. But in the case of a continuous stirred tank reactor (CSTR), the mass constraint manifests through the stock concentrations and in this case
In most model reductions, one assumes stoichiometrically balanced inputs and initial conditions, simplifying the ODEs. But experimentally, it may not be the case, and one may have a limiting reagent and mathematically have an additional parameter in the ODEs. In this case, the mass constraint enters as a parameter, as seen in Eqs. (23)–(25), and all the initial conditions are related by the constraint belonging to the same manifold. In summary, under experimental conditions, one may need to pay attention to the stock solutions and the initial concentrations and include them in the ODEs associated with a particular mechanism.
Acknowledgments
One of the authors (EPL) would like to thank Dr. Nathaniel Wagner and Professor Gonen Ashkenasy for their hospitality during his sabbatical visit to the Department of Chemistry of the Ben Gurion University of the Negev and for countless discussions on chemical self-replications. Their camaraderie made his visit a delightful and productive experience. He would also thank Williams College for its financial support.
Author contributions
Anuj K Shah: Formal analysis(lead);Methodology (equal);Writing-original draft(equal). Enrique Peacock-López: Funding acquisition; Writing -review, and editing (equal).
Data availability
The data supporting this study’s findings are available from the corresponding author upon reasonable request.
References
- 1.
Lee DH, Granja JR, Martinez JA, Severin K, Ghadiri MR. A self-replicating peptide. Nature. 1996; 382 :525-528 - 2.
Severin K, Lee DH, Martinez JA, Ghadiri MR. Peptide self-replication via template-directed ligation. Chemistry - A European Journal. 1997; 3 :1017-1024 - 3.
Lee DH, Severin KK, Ghadiri MR. Autocatalytic networks: The transition from molecular. Current Opinion in Chemical Biology. 1997; 1 :491-496 - 4.
Lee DH, Severin K, Yokobayashi Y, Ghadiri MR. Emergence of symbiosis in peptide self-replication through a hypercyclic network. Nature. 1997; 390 :591-594 - 5.
Yao S, Gossh I, Zutshi R, Chmielewski J. A pH-modulated, self-replicating peptide. Journal of the American Chemical Society. 1997; 119 :10559-10560 - 6.
Yao S, Ghosh I, Zutshi R, Chmielewski J. Selective amplification by auto- and cross-catalysis in a replicating peptide system. Nature. 1998; 396 :447-450 - 7.
Luther A, Brandsch R, von Kiedrowski G. Surface-promoted replication and exponential amplification of DNA analogues. Nature. 1998; 386 :245-248 - 8.
Saghatalias AK, Yokobayashi Y, Soltani K, Ghadiri MR. A chiroselective peptide replicator. Nature. 2001; 409 :797-801 - 9.
Issac R, Ham Y-W, Chmielewski J. The design of self-replicating helical peptides. Current Opinion in Structural Biology. 2001; 11 :458-463 - 10.
Issac R, Chmielewski J. Approaching exponential growth with a self-replicating peptide. Journal of American Chemical Society. 2002; 124 :6808-6809 - 11.
von Kiedrowski G. A self-replicating hexadeoxynucleotide. Angewandte Chemie. 1986; 25 :932-935 - 12.
Zielinski WS, Orgel LE. Autocatalytic synthesis of a tetranucleotide analogue. Nature. 1987; 327 :346-347 - 13.
Szathmary E. Sub-exponential growth and coexistence of non-enzymatically replicating templates. Journal of Theoretical Biology. 1989; 138 :55-58 - 14.
Bag B, von Kiedrowski G. Templates, autocatalytic and molecular replication. Pure and Applied Chemistry. 1996; 68 :2145-2152 - 15.
Assouline S, Nir S, Lahav N. Simulation of non-enzymatic template-directed synthesis of oligonucleotides and peptides. Journal of Theoretical Biology. 2001; 208 :117-125 - 16.
Paul N, Joyce GF. A self-replicating ligase ribozyme. Proceedings of the Natlional Academy Science USA. 2002; 99 :12733-12740 - 17.
Paul N, Jouce GF. Minimal self-replicating systems. Current Opinion in Chemical Biology. 2004; 8 :634-639 - 18.
Lincoln TA, Joyce GF. Self-Sustained replication of RNA. Enzyme Science. 2009; 323 :1229 - 19.
Luebke KJ, Dervan PB. Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation. - 20.
Maher LJ, Dervan PB, Wold B. Kinetic analysis of oligodeoxyribonucleotide-directed triple-helix formation on DNA. Journal of Biochemistry. 1990; 29 :8820 - 21.
Kanavarioti A. Self-replication of chemical sysytems based on recognition within a double or triple helix: A realistic hypothesis. Journal of Theoretical Biology. 1992; 158 :207-219 - 22.
Li T, Nicolaou KC. Chemical self-replication of palindromic duplex DNA. Nature. 1994; 369 :218-221 - 23.
Li T, Weinstein S, Nicolau KC. The chemical end-ligation of homopyrimidine oligodeoxyribonucleotides within a DNA triple helix. Journal of Chemical Biology. 1997; 4 :209-214 - 24.
Vasquez KM, Wilson JH. Triplex-directed modification of genes and gene activity. TIBS. 1998; 23 :1-6 - 25.
Ashkenasy G, Ghadiri MR. Boolean logic functions of synthetic peptide network. Journal of the American Chemical Society. 2004; 126 :11140-11141 - 26.
Ashkenasy G, Jagasla R, Yadav M, Ghadiri MR. Design of a directed molecular network. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101 :10877-10877 - 27.
Dadon Z, Wagner N, Ashkenasy G. Road to non-enzyme molecular networks. Angewandte Chemie, International Edition. 2008; 47 :6128-6136 - 28.
Rubinov B, Wagner N, Rapaport H, Ashkenasy G. Self-replicating amphiphilic β-sheet peptides. Angewandte Chemie. 2009; 121 :6811-6814 - 29.
Wagner N, Ashkenasy G. System chemistry: Logic gates, arithmetic units, and network motifs in small networks. Chemistry – A European Journal. 2009; 15 :1765-1775 - 30.
Samiappan M, Dadon Z, Ashkenasy G. Replication NAND gate with light as input and output. Chemical Communication. 2011; bf47 :710-712 - 31.
Wagner N, Rubinov B, Ashkenasy G. β-sheet-induced chirogenesis in polymerization of oligopeptides. Chemical Physics Chemistry. 2011; 12 :2771-2780 - 32.
Ashkenasy G, Dadon Z, Alesebi S, Wagner N, Ashkenasy N. Building logic into peptide networks: Bottom-up and top-down. Israel Journal of Chemistry. 2011; 51 :106-117 - 33.
Rubinov B, Wagner N, Matmor M, Rege O, Ashkenasy N, Ashkenasy G. Transient fibril structures facilitating nonenzymatic self-replication. ACS Nano. 2012; 6 :7893-7901 - 34.
Dadon Z, Samiappan M, Wagner N, Ashkenasy G. Chemical and light triggering of peptide networks under partial thermodynamic control. Chemical Communications. 2012; 48 :1419-1421 - 35.
Tjivikua T, Ballester P, Rebek J Jr. A self-replicating system. Journal of American Chemical Society. 1990; 112 :1249-1250 - 36.
Hong J-I, Fing Q, Rotello V, Rebek J. Competition, cooperation, and mutation: Improving a synthetic replicator by light irradiation. Science. 1992; 255 :848-850 - 37.
Rebek J Jr. Synthetic self-replicating molecules. Scientific American. 1994; 271 :48-55 - 38.
Wintner EA, Morgan Conn M, Rebek J Jr. Studies in molecular replication ACC. Chemical Research. 1994; 27 :198-203 - 39.
Wintner EA, Morgan Conn M, Rebek J Jr. Self-replicating molecules: A second generation. Journal of American Chemical Society. 1994; 116 :8877-8884 - 40.
Rebek J Jr. A template for life, surface and linear rate. Chemical Brittonia. 1994; 30 :286-290 - 41.
Peacock-López E, Radov DB, Flesner CS. Mixed-mode oscillations in a template mechanism. Biophysical Chemistry. 1997; 65 :171-178 - 42.
Tsai LL, Hutchison GH, Peacock-López E. Turing patterns in a self-replicating mechanism with a self-complementarytemplate. The Journal of Chemical Physics. 2000; 113 :2003-2006 - 43.
Peacock-López E. Chemical oscillations: The templator model. The Chemical Educator. 2001; 6 :202-209 - 44.
MacGehee EA, Peacock-López E. An introduction to turing patterns in nonlinear chemical kinetics. The Chemical Educator. 2005; 10 :84-94 - 45.
Beutel KM, Peacock-López E. Chemical oscillations and Turing patterns in a generalized two-variable model of chemical self-replication. The Journal of Chemical Physics. 2006; 125 :024908 - 46.
Beutel KM, Peacock-López E. Chemical oscillations: Two variable models. The Chemical Educator. 2007; 12 :224-235 - 47.
Beutel KM, Peacock-López E. Complex dynamics in a cross-ctalytic self-replication mechanism. The Journal of Chemical Physics. 2007; 126 :125104 - 48.
Chung JM, Peacock-López E. Cross-diffusion in the Templator model of chemical self-replication. Physics Letters A. 2007; 371 :41-47 - 49.
Chung JM, Peacock-López E. Bifurcation diagrams and Turing patterns in a chemical self-replicating reaction-diffusion system with cross-diffusion. The Journal of Chemical Physics. 2007; 127 :174903 - 50.
Lou SJ, Berry AK, Peacock-López E. Modeling square-wave pulse regulation. Dynamical Systems. 2010; 25 :133-143 - 51.
Lou SJ, Peacock-López E. Self-regulation in a minimal model of chemical self-replication. Journal of Biological Physics. 2012; 20 :87-108 - 52.
Cech TR. A model for the RNA-catalyzed replication of RNA. Proceedings of the National Academy of Sciences. United States of America. 1986; 83 :4360-4363 - 53.
Cech TR. Ribozyme self-replication. Nature. 1989; 339 :507-508 - 54.
Orgel LE. Molecular replication. Nature. 1992; 358 :203-209 - 55.
Orgel LE. Unnatural selection in chemical systems. Accounts of Chemical Research. 1995; 28 :109-118 - 56.
Wolfram S. Mathematica Ver. 13.1. New York: Springer-Verlag; 2022