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Neoplastic Transformation and Suppression of Transformation of Human Bronchial Epithelial Cells in vitro

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Neoplastic Transformation in Human Cell Culture

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 25))

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Abstract

We have taken the following strategy to investigate the role of oncogenes in the neoplastic transformation of human bronchial epithelial cells. First, activated protooncogenes that are associated with human lung cancer are identified. Next, these oncogenes are transferred into the progenitor epithelial cells of broncheogenic carcinoma. The preneoplastic and neoplastic cells are then selected out from the putative suppressive normal cells. The tumorigenicity of the cells containing the transfected oncogenes is then determined using the athymic nude mouse assay system. If the transfected or infected cells show increased tumorigenicity, the dysregulation in the molecular controls of growth and terminal differentiation are then investigated. The methods used to investigate tumor suppressor genes involves several different methodologies including production of somatic cell-cell hybrids with tumorigenic and non-tumorigenic cells, and analysis of mutational events in known tumor suppressor genes in human lung carcinoma cell lines and tumors.

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Author information

Authors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Laboratory of Human Carcinogenesis, Bethesda, MD, 20892, USA

    Teresa A. Lehman & Curtis C. Harris

Authors
  1. Teresa A. Lehman
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  2. Curtis C. Harris
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Editor information

Editors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD, USA

    Johng S. Rhim

  2. Department of Radiation Medicine, Georgetown University Medical Center, Washington DC, USA

    Anatoly Dritschilo

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© 1991 Springer Science+Business Media New York

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Lehman, T.A., Harris, C.C. (1991). Neoplastic Transformation and Suppression of Transformation of Human Bronchial Epithelial Cells in vitro . In: Rhim, J.S., Dritschilo, A. (eds) Neoplastic Transformation in Human Cell Culture. Experimental Biology and Medicine, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0411-4_25

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  • DOI: https://doi.org/10.1007/978-1-4612-0411-4_25

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6750-8

  • Online ISBN: 978-1-4612-0411-4

  • eBook Packages: Springer Book Archive

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