Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Parallel Human Differentiation Into Sensory Neural Subtypes
Publication: Cellular Reprogramming (Formerly "Cloning and Stem Cells")
Volume 19, Issue Number 2
Abstract
The pig is the large animal model of choice for study of nerve regeneration and wound repair. Availability of porcine sensory neural cells would conceptually allow for analogous cell-based peripheral nerve regeneration in porcine injuries of similar severity and size to those found in humans. After recently reporting that porcine (or pig) induced pluripotent stem cells (piPSCs) differentiate into neural rosette (NR) structures similar to human NRs, here we demonstrate that pig NR cells could differentiate into neural crest cells and other peripheral nervous system-relevant cell types. Treatment with either bone morphogenetic protein 4 or fetal bovine serum led to differentiation into BRN3A-positive sensory cells and increased expression of sensory neuron TRK receptor gene family: TRKA, TRKB, and TRKC. Porcine sensory neural cells would allow determination of parallels between human and porcine cells in response to noxious stimuli, analgesics, and reparative mechanisms. In vitro differentiation of pig sensory neurons provides a novel model system for neural cell subtype specification and would provide a novel platform for the study of regenerative therapeutics by elucidating the requirements for innervation following injury and axonal survival.
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Published In
Cellular Reprogramming (Formerly "Cloning and Stem Cells")
Volume 19 • Issue Number 2 • April 2017
Pages: 88 - 94
PubMed: 28266869
Copyright
Copyright 2017, Mary Ann Liebert, Inc.
History
Published in print: April 2017
Published online: 1 April 2017
Published ahead of print: 7 March 2017
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The authors declare that no competing financial interests exist.
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