KEGG ENZYME: 6.2.1.7

ENZYME: 6.2.1.7

Entry

EC 6.2.1.7                  Enzyme

Name

cholate---CoA ligase;
BAL;
bile acid CoA ligase;
bile acid coenzyme A ligase;
choloyl-CoA synthetase;
choloyl coenzyme A synthetase;
cholic thiokinase;
cholate thiokinase;
cholic acid:CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase;
THCA-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate---CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming);
cholyl-CoA synthetase;
trihydroxycoprostanoyl-CoA synthetase

Class

Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases

Sysname

cholate:CoA ligase (AMP-forming)

Reaction(IUBMB)

(1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA [RN:R02794];
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA [RN:R04580]

Reaction(KEGG)

R02794 R04580;
(other) R04507 R08733 R08738 R08743

Substrate

ATP [CPD:C00002];
cholate [CPD:C00695];
CoA [CPD:C00010];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
choloyl-CoA [CPD:C01794];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA

Comment

Requires Mg2+ for activity. The mammalian enzyme is membrane-bound and catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [7]. The bacterial enzyme is soluble and participates in an anaerobic bile acid 7 alpha-dehydroxylation pathway [5].

History

EC 6.2.1.7 created 1961 (EC 6.2.1.29 created 1992, incorporated 2005), modified 2005

Pathway

ec00120

Primary bile acid biosynthesis

ec00121

Secondary bile acid biosynthesis

ec01100

Metabolic pathways

Orthology

K08748

solute carrier family 27 (fatty acid transporter), member 5

K15868

bile acid-coenzyme A ligase

Genes

HSA:	10998(SLC27A5)

PTR:	456351(SLC27A5)

PPS:	100973518(SLC27A5)

GGO:	101141402(SLC27A5)

PON:	100450572(SLC27A5)

NLE:	100598523(SLC27A5)

HMH:	116479284(SLC27A5)

MCC:	713713(SLC27A5)

MCF:	102139611(SLC27A5)

MTHB:

126941889

MNI:	105488041(SLC27A5)

CSAB:

103235458(SLC27A5)

CATY:

105593284(SLC27A5)

PANU:

101012361(SLC27A5)

TGE:	112612525(SLC27A5)

MLEU:

105531198(SLC27A5)

RRO:	104680867(SLC27A5)

RBB:	108514368(SLC27A5)

TFN:	117091868(SLC27A5)

PTEH:

111530345(SLC27A5)

CANG:

105524030(SLC27A5)

CJC:	100394591(SLC27A5)

SBQ:	101033269(SLC27A5)

CIMI:

108289969(SLC27A5)

CSYR:

103262310(SLC27A5)

MMUR:

105864502(SLC27A5)

LCAT:

123624765(SLC27A5)

PCOQ:

105819607(SLC27A5)

OGA:	100943998(SLC27A5)

MMU:	26459(Slc27a5)

MCAL:

110297223(Slc27a5)

MPAH:

110336881(Slc27a5)

RNO:	79111(Slc27a5)

MCOC:

116101654(Slc27a5)

ANU:	117700681(Slc27a5)

MUN:	110562939(Slc27a5)

CGE:	100765567(Slc27a5)

MAUA:

101824762(Slc27a5)

PROB:

127238975(Slc27a5)

PLEU:

114703883(Slc27a5)

MORG:

121449385(Slc27a5)

MFOT:

126493814

AAMP:

119820463(Slc27a5)

NGI:	103725476(Slc27a5)

HGL:	101724180(Slc27a5)

CPOC:

100717817(Slc27a5)

CCAN:

109683037(Slc27a5)

DORD:

105994312(Slc27a5)

DSP:	122110673(Slc27a5)

PLOP:

125338761(Slc27a5)

NCAR:

124966722

MMMA:

107158986(Slc27a5)

OCU:

100340287

OPI:	101527096(SLC27A5)

TUP:	102483776(SLC27A5)

GVR:	103595548(SLC27A5)

CFA:	608675(SLC27A5)

CLUD:

112643327(SLC27A5)

VVP:	112908791(SLC27A5)

VLG:	121484156(SLC27A5)

NPO:	129500979(SLC27A5)

AML:	100478693(SLC27A5)

UMR:	103677216(SLC27A5)

UAH:	113248017(SLC27A5)

UAR:	123776624(SLC27A5)

ELK:

111139861

LLV:

125089011

MPUF:

101687342(SLC27A5)

MNP:	132005114(SLC27A5)

MLK:	131818144(SLC27A5)

NVS:	122912093(SLC27A5)

ORO:	101381954(SLC27A5)

EJU:	114200965(SLC27A5)

ZCA:	113936449(SLC27A5)

MLX:	117997921(SLC27A5)

NSU:	110581926(SLC27A5)

LWW:	102737843(SLC27A5)

FCA:	101086686(SLC27A5)

PYU:	121023395(SLC27A5)

PCOO:

112850703(SLC27A5)

PBG:	122494649(SLC27A5)

PVIV:

125152405(SLC27A5)

LRUF:

124522780

PTG:	102948587(SLC27A5)

PPAD:

109260425(SLC27A5)

PUC:

125915971

AJU:

106987933

HHV:	120227093(SLC27A5)

BTA:	533016(SLC27A5)

BOM:	102285844(SLC27A5)

BIU:	109571897(SLC27A5)

BBUB:

102396712(SLC27A5)

BBIS:

105001638(SLC27A5)

CHX:	102172839(SLC27A5)

OAS:	101120689(SLC27A5)

BTAX:

128063061(SLC27A5)

ODA:	120871847(SLC27A5)

CCAD:

122420603(SLC27A5)

MBEZ:

129546342(SLC27A5)

SSC:	102158419(SLC27A5)

CFR:	102519177(SLC27A5)

CBAI:

105074455(SLC27A5)

CDK:	105096797(SLC27A5)

VPC:	102538658(SLC27A5)

BACU:

103020672(SLC27A5)

BMUS:

118884939(SLC27A5)

LVE:	103086085(SLC27A5)

OOR:	101280268(SLC27A5)

DLE:	111170038(SLC27A5)

PCAD:

102979842(SLC27A5)

PSIU:

116744807(SLC27A5)

NASI:

112396914(SLC27A5)

ECB:	100051678(SLC27A5)

EPZ:	103567891(SLC27A5)

EAI:	106846663(SLC27A5)

MYB:	102261821(SLC27A5)

MYD:	102751593(SLC27A5)

MMYO:

118657417(SLC27A5)

MLF:	102425294(SLC27A5)

PKL:	118712387(SLC27A5)

EFUS:

103304167(SLC27A5)

MNA:	107539367(SLC27A5)

DRO:	112313581(SLC27A5)

SHON:

118988175(SLC27A5)

AJM:	119051596(SLC27A5)

PDIC:

114511678(SLC27A5)

PHAS:

123825485(SLC27A5)

MMF:	118638221(SLC27A5)

PPAM:

129066277(SLC27A5)

HAI:	109377097(SLC27A5)

RFQ:	117034578(SLC27A5)

PALE:

102890200(SLC27A5)

PGIG:

120607133(SLC27A5)

PVP:	105308648(SLC27A5)

RAY:	107500402(SLC27A5)

MJV:	108408237(SLC27A5)

TOD:	119249800(SLC27A5)

SARA:

101552573(SLC27A5)

SETR:

126027979(SLC27A5)

LAV:	100676206(SLC27A5)

TMU:

101361902

ETF:	101643777(SLC27A5)

DNM:	101438816(SLC27A5)

MDO:	100029941(SLC27A5)

GAS:	123242899(SLC27A5)

SHR:	100916639(SLC27A5)

AFZ:

127556609

PCW:	110220184(SLC27A5)

OAA:	100090562(SLC27A5)

LPIC:

129261741

RPHI:

132721510

KIM:	G3T16_11475 G3T16_16845

SDF:	ACG33_01260

DOE:	DENOEST_3894

PLA:	Plav_1025 Plav_1029 Plav_1034

RBS:	RHODOSMS8_00086(baiB)

BJA:	bll0131(bll0131)

BJU:	BJ6T_01450

BJP:	RN69_00685

BRA:

BRADO6529

BBT:

BBta_1112

BRS:

S23_06710

AOL:

S58_10890

BRC:	BCCGELA001_03190

BIC:	LMTR13_34780

BRO:	BRAD285_6438

BRK:	CWS35_11230

BOT:	CIT37_14150

BRQ:	CIT40_01725

BGQ:	X265_03260

BGZ:	XH91_04175

BSYM:

CIT39_00180

BBET:

F8237_17610

BARH:

WN72_46090

BVZ:	BRAD3257_0642

BEL:	BE61_86530

BDG:	LPJ38_06565

BSEP:

HAP48_0020605

BQB:	J4P68_0013450

BSEI:

KMZ68_04495

BBAN:

J4G43_050000

BCAN:

BcanWSM471_03240 BcanWSM471_08330

BAUT:

QA635_37450

BBRA:

QA636_38525

BGK:	IC762_05100

BCOU:

IC761_03335

BPAH:

QA639_36935

BXN:	I3J27_03165 I3J27_33625 I3J27_33655

BROS:

QUH67_31575

BRAZ:

LRP30_42785

VGO:	GJW-30_1_02490(baiB_2)

TALZ:

RPMA_07725

XDI:	EZH22_21750

RBM:

TEF_16880

CAK:	Caul_3247 Caul_4604

CAUL:

KCG34_17910

PZU:

PHZ_c2517

BNE:	DA69_06025

BREA:

HZ989_08370

TSV:	DSM104635_02995 DSM104635_03075

YAN:	AYJ57_19780

TMD:	KUV46_00545

RSU:

NHU_03725

RHM:	B5V46_17095

HNE:	HNE_0099(baiB)

NGF:	FRF71_13160

SMAZ:

LH19_12220 LH19_27280

SPHO:

C3E99_13085

SWI:	Swit_0351 Swit_1599 Swit_1600

SPHD:

HY78_17585 HY78_17590 HY78_17610 HY78_26355

RDI:	CMV14_03665

SJP:	SJA_C2-03890 SJA_C2-04050

SCH:	Sphch_3661

SPMI:

K663_20463

SPHT:

K426_28370

CIJ:	WG74_11935

NAO:	Y958_27085

CSCI:

HDCHBGLK_01430(baiB)

CHYL:

CE91St63_15040

CBIL:

EUBC25_24900

PHX:	KGNDJEFE_00401(baiB)

ELE:

Elen_3099

MPA:

MAP_2354

MAV:

MAV_1627

MIT:	OCO_16930 OCO_48560

MIA:	OCU_17130 OCU_48510

MID:	MIP_02338 MIP_07342

MYO:	OEM_14930 OEM_48710

MCHI:

AN480_08330 AN480_23315 AN480_25245

MIR:	OCQ_14600 OCQ_49570

MMAL:

CKJ54_07175 CKJ54_23135

MMAN:

MMAN_24730 MMAN_36630

MSA:	Mycsm_00180

MUL:

MUL_3844

MMI:

MMAR_3983

MMAE:

MMARE11_37900

MLI:	MULP_04163

MMC:

Mmcs_0223

MKM:

Mkms_0233

MJL:

Mjls_0213

MMM:	W7S_07140 W7S_24315

MKN:	MKAN_05880

MYE:	AB431_01945

MSHG:

MSG_00422 MSG_03568

MFJ:	MFLOJ_30860 MFLOJ_51280

MSTO:

MSTO_34790 MSTO_55390

MSIM:

MSIM_20450 MSIM_40770

MSAK:

MSAS_18290

MKU:	I2456_02760 I2456_18740

MLW:	MJO58_02270 MJO58_19560

MXE:	MYXE_17320 MYXE_46350

MNV:	MNVI_02600 MNVI_19750

MPAG:

C0J29_02725 C0J29_21705

MGOR:

H0P51_02740 H0P51_19675

MCOO:

MCOO_16060 MCOO_35900

MBAI:

MB901379_03460(lcfB_9)

MSEO:

MSEO_23560 MSEO_43170

MHEK:

JMUB5695_01801 JMUB5695_04479

MGAU:

MGALJ_47310

MLJ:	MLAC_10490

MBRD:

MBRA_12830 MBRA_32660

MMAM:

K3U93_16925

MSPG:

F6B93_16025

MOT:	LTS72_05340

MPAA:

MKK62_07880 MKK62_23045

MKY:	IWGMT90018_04990 IWGMT90018_41340

MWU:	PT015_09070 PT015_19535

MSM:	MSMEG_0318

MSG:	MSMEI_0312

MSB:	LJ00_01600

MSN:	LI99_01600

MSH:	LI98_01600

MVA:

Mvan_0252

MGI:

Mflv_0419

MCB:	Mycch_0222

MFT:	XA26_02940

MPHL:

MPHLCCUG_04868(baiB_2)

MVQ:

MYVA_0257

MLL:	B1R94_01565

MRH:	MycrhN_1496

MTHN:

4412656_00248(fadD_2)

MDU:	MDUV_11370

MCHT:

MCHIJ_07000

MAUU:

NCTC10437_00256

MMOR:

MMOR_01890 MMOR_58370

MAIC:

MAIC_53080

MIJ:	MINS_27090

MTY:	MTOK_10360

MPSC:

MPSYJ_06360

MGAD:

MGAD_45630

MHEV:

MHEL_18710

MSAR:

MSAR_18910

MANY:

MANY_43890

MPOF:

MPOR_07960

MBOK:

MBOE_15230

MFG:	K6L26_30000

MFLV:

NCTC10271_04880

MCEE:

MCEL_29920

MMON:

EWR22_01215

MGO:	AFA91_08425

MHOL:

K3U96_25350

MSEN:

K3U95_01455

MPAE:

K0O64_01570

MRF:	MJO55_01200

MCRO:

MI149_02120

MAB:

MAB_2316

MMV:	MYCMA_1247

MABB:

MASS_2242

MABL:

MMASJCM_2266

MCHE:

BB28_11610

MIZ:	BAB75_12360

MSTE:

MSTE_02250

MSAO:

MYCSP_11375

MSAL:

DSM43276_02035(baiB)

CGY:	CGLY_15810

NBR:	O3I_021420

NNO:	NONO_c50630

NOZ:	DMB37_08020

NYA:	LTV02_22920

RTM:	G4H71_11130

RKO:	JWS14_47605

RHOP:

D8W71_02125

WHR:	OG579_02230

GOM:	D7316_03708(baiB_2)

SBH:

SBI_00137

STRC:

AA958_12300

SNZ:	DC008_01285

SSEO:

D0Z67_25560

SBAT:

G4Z16_17215

SFIY:

F0344_07365

YIA:	LO772_03550 LO772_29980 LO772_35175

CPHA:

FGI33_11885

CCAF:

FGD68_03385

RFS:	C1I64_10215

CART:

PA27867_2220

BRR:	C1N80_03880

CDON:

KKR89_09550

AER:	AERYTH_14445

ACTW:

F7P10_09660 F7P10_11280

AMAZ:

LUW76_14885 LUW76_14955 LUW76_22035 LUW76_25845

SPIQ:

OHA34_14875

NOW:	GBF35_30420

FRE:	Franean1_0614 Franean1_4026

FRI:	FraEuI1c_2666 FraEuI1c_3335 FraEuI1c_4447 FraEuI1c_5318 FraEuI1c_5444

SEN:	SACE_3387(baiB)

AMYY:

YIM_33605(baiB2)

PDX:

Psed_0542

PSEA:

WY02_09705

PSEE:

FRP1_04515

PSEH:

XF36_02965

PSEQ:

AD006_12175

PECQ:

AD017_20000

PAUT:

Pdca_52450

PBRO:

HOP40_02455

PPEL:

H6H00_02995

SSYI:

EKG83_31105

KAL:

KALB_5626

KPHY:

AOZ06_30715

ACTY:

OG774_25295

UME:	RM788_00940 RM788_48095

MICH:

FJK98_11545

VER:	HUT12_13430

PLK:	CIK06_12190

DFU:	Dfulv_32595

EKE:	EK0264_04430

AYM:	YM304_25790

ATQ:	GH723_15305

AQZ:	KSP35_22785

» show all

Reference

1 [PMID:13303991]

Authors

ELLIOTT WH.

Title

The enzymic activation of cholic acid by guinea-pig-liver microsomes.

Journal

Biochem J 62:427-33 (1956)
DOI:10.1042/bj0620427

Reference

2 [PMID:13403911]

Authors

ELLIOTT WH.

Title

The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes.

Journal

Biochem J 65:315-21 (1957)
DOI:10.1042/bj0650315

Reference

3 [PMID:3183523]

Authors

Prydz K, Kase BF, Bjorkhem I, Pedersen JI.

Title

Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver.

Journal

J Lipid Res 29:997-1004 (1988)

Reference

4 [PMID:2521999]

Authors

Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP.

Title

Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver.

Journal

Biochem J 257:221-9 (1989)
DOI:10.1042/bj2570221

Reference

5 [PMID:1551828]

Authors

Mallonee DH, Adams JL, Hylemon PB

Title

The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase.

Journal

J Bacteriol 174:2065-71 (1992)
DOI:10.1128/JB.174.7.2065-2071.1992

Sequence

[ag:AAC45410]

Reference

6 [PMID:9390170]

Authors

Wheeler JB, Shaw DR, Barnes S.

Title

Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.

Journal

Arch Biochem Biophys 348:15-24 (1997)
DOI:10.1006/abbi.1997.0391

Sequence

[rno:79111]

Reference

7 [PMID:12454267]

Authors

Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.

Title

Molecular cloning and expression of rat liver bile acid CoA ligase.

Journal

J Lipid Res 43:2062-71 (2002)
DOI:10.1194/jlr.m200260-jlr200

Sequence

[rno:79111]

Other DBs

ExplorEnz - The Enzyme Database:

6.2.1.7

IUBMB Enzyme Nomenclature:

6.2.1.7

ExPASy - ENZYME nomenclature database:

6.2.1.7

BRENDA, the Enzyme Database:

6.2.1.7

CAS:

9027-90-1

DBGET integrated database retrieval system