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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Home - Search by name - Free text search - Search by properties |
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| Cell line name | Vero |
| Accession number | ICLC ATL95005 |
| Brief description | Species: monkey, African green; Tissue: kidney |
| Description | Species: monkey, African green adult; Tissue: kidney |
| Depositor | Obtained from ECACC. |
| Reference paper | Nippon Rinsho 1963;21:1209 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 1-3x104 cells/cm2. 37C, 5% CO2. |
| Properties | indicator line for mycoplasma testing; virology; virus titration; virus replication; plaque assays; bacterial cytotoxicity.; Susceptible to: poliovirus 3, echovirus, reovirus, rubella, herpes simplex, arbovirus, adenovirus 12, influenza, paramyxovirus, Getah arbovirus, orthomixovirus, African swine fever virus, bluetongue virus, bovine leucosis, orbivirus, porcine epidemic diarrhea virus |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as monkey with AST, MD, NP, PepB |
| Karyology | modal number 58 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | Kitasato Arc Exp Med 1964;37:27-42 - PMID: 5833688 Nature 1967;216:271 Proc Soc Exp Biol Med 1967;125:602 - DOI: 10.3181/00379727-125-32157 - PMID: 4961492 |