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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Home - Search by name - Free text search - Search by properties |
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| Cell line name | COS-7 |
| Accession number | ICLC ATL96005 |
| Brief description | Species: monkey, African green; Tissue: kidney; Transformed by: SV40origin defective mutant; Original line: CV-1 |
| Description | Species: monkey, African green; Tissue: kidney; Transformed by: SV40origin defective mutant; Original line: CV-1 |
| Depositor | Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy |
| Reference paper | Cell 1981;23:175-182 - DOI: 10.1016/0092-8674(81)90282-8 - PMID: 6260373 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | The line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as monkey with MD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |