Abstract
Myosin V is an actin-based motor essential for a variety of cellular processes including skin pigmentation, cell separation and synaptic transmission. Myosin V transports organelles, vesicles and mRNA by binding, directly or indirectly, to cargo-bound receptors via its C-terminal globular tail domain (GTD). We have used the budding yeast myosin V Myo2p to shed light on the mechanism of how Myo2p interacts with post-Golgi carriers. We show that the Rab/Ypt protein Ypt32p, which associates with membranes of the trans-Golgi network, secretory vesicles and endosomes and is related to the mammalian Rab11, interacts with the Myo2p GTD within a region previously identified as the 'vesicle binding region'. Furthermore, we show that the essential myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p. Our data are consistent with a role of Ypt32p and Mlc1p in regulating the interaction of post-Golgi carriers with Myo2p subdomain II.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Amino Acid Motifs
- Amino Acid Sequence
- Amino Acid Substitution
- Conserved Sequence
- Gene Expression Regulation, Fungal
- Molecular Sequence Data
- Molecular Structure
- Myosin Heavy Chains / chemistry
- Myosin Heavy Chains / genetics
- Myosin Heavy Chains / metabolism*
- Myosin Light Chains / metabolism
- Myosin Type V / chemistry
- Myosin Type V / genetics
- Myosin Type V / metabolism*
- Protein Binding
- Protein Structure, Tertiary
- Saccharomyces cerevisiae / genetics
- Saccharomyces cerevisiae / metabolism*
- Saccharomyces cerevisiae Proteins / chemistry
- Saccharomyces cerevisiae Proteins / genetics
- Saccharomyces cerevisiae Proteins / metabolism*
- Secretory Vesicles / physiology*
- Secretory Vesicles / ultrastructure
- Sequence Alignment
- Two-Hybrid System Techniques
- rab GTP-Binding Proteins / genetics
- rab GTP-Binding Proteins / metabolism*
Substances
- MLC1 protein, S cerevisiae
- MYO2 protein, S cerevisiae
- Myosin Light Chains
- Saccharomyces cerevisiae Proteins
- Myosin Type V
- YPT32 protein, S cerevisiae
- Myosin Heavy Chains
- rab GTP-Binding Proteins