Abstract
Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA levels. Clusters of circadian-regulated genes were found in pathways involved in plant responses to light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.
Publication types
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Arabidopsis / genetics
- Arabidopsis / growth & development
- Arabidopsis / physiology*
- Biological Clocks / genetics*
- Carbohydrate Metabolism
- Circadian Rhythm*
- Gene Expression Profiling
- Gene Expression Regulation, Plant*
- Genes, Plant
- Light
- Nitrogen / metabolism
- Oligonucleotide Array Sequence Analysis
- Photosynthesis / genetics
- Photosynthetic Reaction Center Complex Proteins / genetics
- Plants, Genetically Modified
- Promoter Regions, Genetic
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
- RNA, Plant / genetics
- RNA, Plant / metabolism
- Sulfur / metabolism
- Transcription, Genetic*
Substances
- Photosynthetic Reaction Center Complex Proteins
- RNA, Messenger
- RNA, Plant
- Sulfur
- Nitrogen