Abstract
Canine distemper virus (CDV) and rabies virus (RV) are two important pathogens of the dog. CDV, a member of the morbillivirus genus, has shown promise as an expression vector. The glycoprotein from RV is a main contributor to protective immunity and capable of eliciting the production of virus-neutralizing antibodies. In this study, we recovered an attenuated strain of canine distemper virus and constructed a recombinant virus, rCDV-RV-G, expressing a modified (R333Q) rabies virus glycoprotein (RV-G) of RV Flury strain LEP. RV-G expression by the recombinant viruses was confirmed. Furthermore, G was proved to be incorporated into the surface of CDV particles. While replication of the recombinant virus was slightly reduced compared with the parental CDV, it stably expressed the RV-G over ten serial passages. Inoculation of mice induced specific neutralizing antibodies against both RV-G and CDV. Therefore, the rCDV-RV-G has the potential as a vaccine that may be used to control rabies virus infection in dogs and other animals.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Animals
- Antibodies, Neutralizing / blood
- Antibodies, Viral / blood
- Antigens, Viral / genetics
- Antigens, Viral / immunology*
- Distemper Virus, Canine / genetics*
- Dogs
- Drug Carriers*
- Glycoproteins / genetics
- Glycoproteins / immunology*
- Mice
- Rabies Vaccines / administration & dosage
- Rabies Vaccines / genetics
- Rabies Vaccines / immunology*
- Rabies virus / genetics
- Rabies virus / immunology*
- Recombinant Proteins / genetics
- Recombinant Proteins / immunology
- Vaccines, Attenuated / administration & dosage
- Vaccines, Attenuated / genetics
- Vaccines, Attenuated / immunology
- Vaccines, Synthetic / administration & dosage
- Vaccines, Synthetic / genetics
- Vaccines, Synthetic / immunology
- Viral Envelope Proteins / genetics
- Viral Envelope Proteins / immunology*
Substances
- Antibodies, Neutralizing
- Antibodies, Viral
- Antigens, Viral
- Drug Carriers
- Glycoproteins
- Rabies Vaccines
- Recombinant Proteins
- Vaccines, Attenuated
- Vaccines, Synthetic
- Viral Envelope Proteins
- glycoprotein G, Rabies virus