Parent Class:

EC-Numbers → 1 -- Oxidoreductases → 1.11 -- Acting on a peroxide as acceptor → 1.11.1 -- Peroxidases

Synonyms: VP, hybrid peroxidase, polyvalent peroxidase, reactive-black-5:hydrogen-peroxide oxidoreductase

Systematic Name: 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol:hydrogen-peroxide oxidoreductase

Unification Links: BRENDA:1.11.1.16, ENZYME:1.11.1.16, IUBMB-ExplorEnz:1.11.1.16

Reaction:
2 Mn²⁺ + hydrogen peroxide + 2 H⁺ → 2 Mn³⁺ + 2 H₂O,
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + hydrogen peroxide → vanillin + guaiacol + glycolaldehyde + H₂O

Unofficial Reactions:
reduced reactive black 5 + hydrogen peroxide → oxidized reactive black 5 + 2 H₂O

Enzymes and Genes:
versatile peroxidase VPL2: vpl2 ( Pleurotus eryngii )
versatile peroxidase VPL1: vpl1 ( Pleurotus eryngii )

Summary:
This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC 1.11.1.13, manganese peroxidase and EC 1.11.1.14, lignin peroxidase. It is also able to oxidize phenols, hydroquinones and both low- and high redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [Heinfling98, Camarero99].

Citations: [Martinez96, RuizDuenas99, RuizDuenas99a, Caramelo99, Camarero00, RuizDuenas01, Banci03, PerezBoada05]

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References

Banci03: Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ (2003). "NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii." J Biol Inorg Chem 8(7);751-60. PMID: 12884090

Camarero00: Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT (2000). "The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases." FEMS Microbiol Lett 191(1);37-43. PMID: 11004397

Camarero99: Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites." J Biol Chem 274(15);10324-30. PMID: 10187820

Caramelo99: Caramelo L, Martinez MJ, Martinez AT (1999). "A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers." Appl Environ Microbiol 65(3);916-22. PMID: 10049842

Heinfling98: Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT (1998). "A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta." FEBS Lett 428(3);141-6. PMID: 9654123

Martinez96: Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT (1996). "Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii." Eur J Biochem 237(2);424-32. PMID: 8647081

PerezBoada05: Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT (2005). "Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways." J Mol Biol 354(2);385-402. PMID: 16246366

RuizDuenas01: Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT (2001). "A new versatile peroxidase from Pleurotus." Biochem Soc Trans 29(Pt 2);116-22. PMID: 11356138

RuizDuenas99: Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii." Mol Microbiol 31(1);223-35. PMID: 9987124

RuizDuenas99a: Ruiz-Duenas FJ, Martinez MJ, Martinez AT (1999). "Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates." Appl Environ Microbiol 65(10);4705-7. PMID: 10508113

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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