Synonyms: protoporphyrinogen IX oxidase, protoporphyrinogenase, PPO, Protox, HemG, HemY
Systematic Name: protoporphyrinogen-IX:oxygen oxidoreductase
Unification Links: BRENDA:1.3.3.4, ENZYME:1.3.3.4, IUBMB-ExplorEnz:1.3.3.4
Reaction:
protoporphyrinogen IX + 3 dioxygen → protoporphyrin IX + 3 hydrogen peroxide
Enzymes and Genes:
protoporphyrinogen oxidase: hemJ ( Synechocystis sp. PCC 6803 )
protoporphyrinogen oxidase: PPOX ( Homo sapiens )
protoporphyrinogen IX oxidase isozyme II: PPXII ( Nicotiana tabacum )
protoporphyrinogen oxidase: PPOX ( Arabidopsis thaliana col )
Summary:
This is the last common enzyme in the biosynthesis of chlorophylls and heme [Che00]. Two isoenzymes exist in plants: one in plastids and the other in mitochondria. This is the target enzyme of phthalimide-type and diphenylether-type herbicides [Che00]. The enzyme from oxygen-dependent species contains FAD [Dailey98]. Also slowly oxidizes mesoporphyrinogen IX.
Citations: [Poulson75, Poulson76, Ferreira88, Dailey96, Dailey96a, Wang01]
References
Che00: Che FS, Watanabe N, Iwano M, Inokuchi H, Takayama S, Yoshida S, Isogai A (2000). "Molecular characterization and subcellular localization of protoporphyrinogen oxidase in spinach chloroplasts." Plant Physiol 124(1);59-70. PMID: 10982422
Dailey96: Dailey TA, Dailey HA (1996). "Human protoporphyrinogen oxidase: expression, purification, and characterization of the cloned enzyme." Protein Sci 5(1);98-105. PMID: 8771201
Dailey96a: Dailey HA, Dailey TA (1996). "Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme." J Biol Chem 271(15);8714-8. PMID: 8621504
Dailey98: Dailey TA, Dailey HA (1998). "Identification of an FAD superfamily containing protoporphyrinogen oxidases, monoamine oxidases, and phytoene desaturase. Expression and characterization of phytoene desaturase of Myxococcus xanthus." J Biol Chem 273(22);13658-62. PMID: 9593705
Ferreira88: Ferreira GC, Dailey HA (1988). "Mouse protoporphyrinogen oxidase. Kinetic parameters and demonstration of inhibition by bilirubin." Biochem J 250(2);597-603. PMID: 2451512
Poulson75: Poulson R, Polglase WJ (1975). "The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX. Protoporphyrinogen oxidase activity in mitochondrial extracts of Saccharomyces cerevisiae." J Biol Chem 250(4);1269-74. PMID: 234450
Poulson76: Poulson R (1976). "The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX in mammalian mitochondria." J Biol Chem 251(12);3730-3. PMID: 6461
Wang01: Wang KF, Dailey TA, Dailey HA (2001). "Expression and characterization of the terminal heme synthetic enzymes from the hyperthermophile Aquifex aeolicus." FEMS Microbiol Lett 202(1);115-9. PMID: 11506917
Report Errors or Provide Feedback Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018 Page generated by Pathway Tools version 28.0 (software by SRI International) on Sat Apr 27, 2024, BIOCYC14A.
hide 
show operations 
