MetaCyc

Parent Class:

EC-Numbers → 1 -- Oxidoreductases → 1.5 -- Acting on the CH-NH group of donors → 1.5.1 -- With NAD(+) or NADP(+) as acceptor

Synonyms: N⁵,N¹⁰-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate:NADP oxidoreductase, 5,10-methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase (NADP)

Systematic Name: 5,10-methylenetetrahydrofolate:NADP⁺ oxidoreductase

Unification Links: BRENDA:1.5.1.5, ENZYME:1.5.1.5, IUBMB-ExplorEnz:1.5.1.5

Reaction:
a 5,10-methylenetetrahydrofolate + NADP⁺ ↔ a 5,10-methenyltetrahydrofolate + NADPH

Enzymes and Genes:
bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase: folD ( Escherichia coli K-12 substr. MG1655 )
methylenetetrahydrofolate dehydrogenase [multifunctional]: folD ( Bacillus methanolicus )
methylenetetrahydrofolate dehydrogenase ( Clostridium cylindrosporum )
C1-THF synthase: MTHFD1 ( Homo sapiens )
5,10-methylenetetrahydrofolate dehydrogenase [multifunctional]: AF030516 ( Pisum sativum )
methenyl tetrahydrofolate cyclohydrolase [multifunctional]: folD ( Hyphomicrobium zavarzinii ZV580 )
NADP-dependent methylene tetrahydromethanopterin dehydrogenase [multifunctional]: mtdA ( Methylorubrum extorquens AM1 )
methenyltetrahydrofolate cyclohydrolase [multifunctional]: folD ( Mycoplasmoides pneumoniae M129 )
methenyltetrahydrofolate cyclohydrolase [multifunctional]: folD ( Moorella thermoacetica )
methenyltetrahydrofolate cyclohydrolase [multifunctional]: folD ( Desulfovibrio desulfuricans )

Summary:
This reaction is involved in folic acid synthesis and is necessary for the biosynthesis of purines, thymydylate, methionine, histidine, pantothenate and formyl tRNA-MET. cf EC 1.5.1.15, methylenetetrahydrofolate dehydrogenase (NAD⁺).

Citations: [Osborn57, Hatefi57, Ramasastri62, Yeh65]