Selective localization of myosin-I proteins in macropinosomes and actin waves
Corresponding Author
Hanna Brzeska
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Address correspondence to: Hanna Brzeska, National Institutes of Health, Bldg. 50, Rm. 2515, 9000 Rockville Pike, Bethesda, MD 20892, USA. E-mail: [email protected]Search for more papers by this authorHilary Koech
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorKevin J. Pridham
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorEdward D. Korn
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorMargaret A. Titus
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota
Search for more papers by this authorCorresponding Author
Hanna Brzeska
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Address correspondence to: Hanna Brzeska, National Institutes of Health, Bldg. 50, Rm. 2515, 9000 Rockville Pike, Bethesda, MD 20892, USA. E-mail: [email protected]Search for more papers by this authorHilary Koech
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorKevin J. Pridham
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorEdward D. Korn
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
Search for more papers by this authorMargaret A. Titus
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota
Search for more papers by this authorMonitoring Editor: Bruce Goode
Abstract
Class I myosins are widely expressed with roles in endocytosis and cell migration in a variety of cell types. Dictyostelium express multiple myosin Is, including three short-tailed (Myo1A, Myo1E, Myo1F) and three long-tailed (Myo1B, Myo1C, Myo1D). Here we report the molecular basis of the specific localizations of short-tailed Myo1A, Myo1E, and Myo1F compared to our previously determined localization of long-tailed Myo1B. Myo1A and Myo1B have common and unique localizations consistent with the various features of their tail region; specifically the BH sites in their tails are required for their association with the plasma membrane and heads are sufficient for relocalization to the front of polarized cells. Myo1A does not localize to actin waves and macropinocytic protrusions, in agreement with the absence of a tail region which is required for these localizations of Myo1B. However, in spite of the overall similarity of their domain structures, the cellular distributions of Myo1E and Myo1F are quite different from Myo1A. Myo1E and Myo1F, but not Myo1A, are associated with macropinocytic cups and actin waves. The localizations of Myo1E and Myo1F in macropinocytic structures and actin waves differ from the localization of Myo1B. Myo1B colocalizes with F-actin in the actin waves and at the tips of mature macropinocytic cups whereas Myo1E and Myo1F are in the interior of actin waves and along the entire surface of macropinocytic cups. Our results point to different mechanisms of targeting of short- and long-tailed myosin Is, and are consistent with these myosins having both shared and divergent cellular functions. © 2016 Wiley Periodicals, Inc.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
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cm21275-sup-0001-suppfigs1.tif5.9 MB | Supporting Figure S1 |
cm21275-sup-0002-suppfigs2.tif2.9 MB | Supporting Figure S2 |
cm21275-sup-0003-suppfigs3.tif4.2 MB | Supporting Figure S3 |
cm21275-sup-0004-suppfigs4.tif7 MB | Supporting Figure S4 |
cm21275-sup-0005-suppmovie1.mov5.3 MB | Supporting Movie 1 |
cm21275-sup-0006-suppmovie2.mov5.1 MB | Supporting Movie 2 |
cm21275-sup-0007-suppmovie3.mov2.5 MB | Supporting Movie 3 |
cm21275-sup-0008-suppmovie4.mov7.8 MB | Supporting Movie 4 |
cm21275-sup-0009-suppmovie5.mov1.8 MB | Supporting Movie 5 |
cm21275-sup-0010-suppinfo.docx24.5 KB | Supporting Information |
cm21275-sup-0011-supptbl1.docx14.8 KB | Supporting Table 1 |
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