Association of the yeast poly(A) tail binding protein with translation initiation factor eIF‐4G.
The EMBO Journal
(1996)
15: 7168 - 7177
Although the cap structure and the poly(A) tail are on opposite ends of the mRNA molecule, previous work has suggested that they interact to enhance translation and inhibit mRNA degradation. Here we present biochemical data that show that the proteins bound to the mRNA cap (eIF‐4F) and poly(A) tail (Pab1p) are physically associated in extracts from the yeast Saccharomyces cerevisiae. Specifically, we find that Pab1p co‐purifies and co‐immunoprecipitates with the eIF‐4G subunit of eIF‐4F. The Pab1p binding site on the recombinant yeast eIF‐4G protein Tif4632p was mapped to a 114‐amino‐acid region just proximal to its eIF‐4E binding site. Pab1p only bound to this region when complexed to poly(A). These data support the model that the Pablp‐poly(A) tail complex on mRNA can interact with the cap structure via eIF‐4G.
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Published in issue: December 1996
Published online: 16 December 1996
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© 1996 European Molecular Biology Organization.
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- Sam Kajjo, Sahil Sharma, Shan Chen, William R Brothers, Megan Cott, Benedeta Hasaj, Predrag Jovanovic, Ola Larsson, Marc R Fabian, PABP prevents the untimely decay of select mRNA populations in human cells, The EMBO Journal, 10.15252/embj.2021108650, 41, 6, (2022).
- Boris Slobodin, Rivka Dikstein, So close, no matter how far: multiple paths connecting transcription to mRNA translation in eukaryotes, EMBO reports, 10.15252/embr.202050799, 21, 9, (2020).
- Hila Gingold, Yitzhak Pilpel, Determinants of translation efficiency and accuracy, Molecular Systems Biology, 10.1038/msb.2011.14, 7, 1, (2011).
- Eun‐Hee Park, Sarah E Walker, Joseph M Lee, Stefan Rothenburg, Jon R Lorsch, Alan G Hinnebusch, Multiple elements in the eIF4G1 N‐terminus promote assembly of eIF4G1•PABP mRNPs in vivo, The EMBO Journal, 10.1038/emboj.2010.312, 30, 2, (302-316), (2011).
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- Mohammad A Mir, Antonito T Panganiban, A protein that replaces the entire cellular eIF4F complex, The EMBO Journal, 10.1038/emboj.2008.228, 27, 23, (3129-3139), (2008).
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