Single Chain Epidermal Growth Factor Receptor Antibody Conjugated Nanoparticles for in vivo Tumor Targeting and Imaging
Corresponding Author
Lily Yang
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Lily Yang, Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA).
Shuming Nie, Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA).
Search for more papers by this authorHui Mao
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorY. Andrew Wang
Ocean Nanotech, LLC Fayetteville, AR 72701 (USA)
Search for more papers by this authorZehong Cao
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXianghong Peng
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXiaoxia Wang
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorHongwei Duan
Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA)
Search for more papers by this authorChunchun Ni
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorQingan Yuan
Fox Chase Cancer Center Philadelphia, PA 19111 (USA)
Search for more papers by this authorGregory Adams
Fox Chase Cancer Center Philadelphia, PA 19111 (USA)
Search for more papers by this authorMark Q. Smith
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorWilliam C. Wood
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXiaohu Gao
Department of Bioengineering University of Washington Seattle, WA 98195 (USA)
Search for more papers by this authorCorresponding Author
Shuming Nie
Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA)
Lily Yang, Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA).
Shuming Nie, Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA).
Search for more papers by this authorCorresponding Author
Lily Yang
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Lily Yang, Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA).
Shuming Nie, Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA).
Search for more papers by this authorHui Mao
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorY. Andrew Wang
Ocean Nanotech, LLC Fayetteville, AR 72701 (USA)
Search for more papers by this authorZehong Cao
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXianghong Peng
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXiaoxia Wang
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorHongwei Duan
Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA)
Search for more papers by this authorChunchun Ni
Department of Radiology Emory University School of Medicine EUH AG11, 1364 Clifton Road, Atlanta, GA 30322 (USA)
Search for more papers by this authorQingan Yuan
Fox Chase Cancer Center Philadelphia, PA 19111 (USA)
Search for more papers by this authorGregory Adams
Fox Chase Cancer Center Philadelphia, PA 19111 (USA)
Search for more papers by this authorMark Q. Smith
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorWilliam C. Wood
Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA)
Search for more papers by this authorXiaohu Gao
Department of Bioengineering University of Washington Seattle, WA 98195 (USA)
Search for more papers by this authorCorresponding Author
Shuming Nie
Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA)
Lily Yang, Department of Surgery, Winship Cancer Institute Emory University School of Medicine 1365 C Clifton Road NE Atlanta, GA 30322 (USA).
Shuming Nie, Department of Biomedical Engineering Emory University School of Medicine 101 Woodruff Circle, Suite 2007B, Atlanta, GA 30322 (USA).
Search for more papers by this authorAbstract
Epidermal growth factor receptor (EGFR) targeted nanoparticle are developed by conjugating a single-chain anti-EGFR antibody (ScFvEGFR) to surface functionalized quantum dots (QDs) or magnetic iron oxide (IO) nanoparticles. The results show that ScFvEGFR can be successfully conjugated to the nanoparticles, resulting in compact ScFvEGFR nanoparticles that specifically bind to and are internalized by EGFR-expressing cancer cells, thereby producing a fluorescent signal or magnetic resonance imaging (MRI) contrast. In vivo tumor targeting and uptake of the nanoparticles in human cancer cells is demonstrated after systemic delivery of ScFvEGFR-QDs or ScFvEGFR-IO nanoparticles into an orthotopic pancreatic cancer model. Therefore, ScFvEGFR nanoparticles have potential to be used as a molecular-targeted in vivo tumor imaging agent. Efficient internalization of ScFvEGFR nanoparticles into tumor cells after systemic delivery suggests that the EGFR-targeted nanoparticles can also be used for the targeted delivery of therapeutic agents.
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