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Involvement of early growth response-2 (Egr-2) in lipopolysaccharide-induced neuroinflammation

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Abstract

Early growth response-2 (Egr-2) protein is a transcription factor, which belongs to Egr family which involve in modulating the peripheral immune response, by means of the induction of differentiation of lymphocyte precursors, activation of T and B cells. Egr-2 plays essential roles in peripheral nerve myelination, adipogenesis, tissue repair and fibrosis, immune tolerance; however, its regulation and role in central nervous system (CNS) remain poorly understood. In contrast to Egr-1, which has been extensively investigated, the regulation and function of Egr-2 remains less well characterized. To elaborate whether Egr-2 was involved in CNS injury, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats. Egr-2 expression was strongly induced in active glia cells (astrocytes and microglias) in inflamed brain cortex. In vitro studies indicated that the upregulation of Egr-2 may be involved in the subsequent glia cellular activation following LPS exposure; and knock down of Egr-2 in primary mixed glial cultures (MGC) by siRNA showed that Egr-2 promoted the synthesis of TNF-α. Collectively, these results suggested Egr-2 may be important in host defense in CNS immune response, which might provide a potential target to the treatment of neuroinflammation.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81070992) and funded by Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Nantong University graduate scientific and technological innovation projects (No. YKC12030).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Yaohua Yan, Xiang Tan, Xinmin Wu, Bai Shao, Xiaohong Wu, Jianhua Cao, Jian Xu, Wei Jin, Lei Li, Wei Xu & Yilu Gao

  2. Department of Immunology, Basic Medical College, Hubei University of Science and Technology, Xianning, Hubei Province, 437000, People’s Republic of China

    Xijun Wang

  3. Department of Neurology, The First Affiliated Hospital of Suzhou University, Suzhou, Jiangsu Province, 215000, People’s Republic of China

    Gang Cui

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  1. Yaohua Yan
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  2. Xiang Tan
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  3. Xinmin Wu
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  4. Bai Shao
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  5. Xiaohong Wu
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  6. Jianhua Cao
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  7. Jian Xu
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  8. Wei Jin
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  9. Lei Li
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  10. Wei Xu
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  11. Xijun Wang
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  12. Yilu Gao
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  13. Gang Cui
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Corresponding authors

Correspondence to Yilu Gao or Gang Cui.

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Yaohua Yan and Xiang Tan contributed equally to this work.

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Yan, Y., Tan, X., Wu, X. et al. Involvement of early growth response-2 (Egr-2) in lipopolysaccharide-induced neuroinflammation. J Mol Hist 44, 249–257 (2013). https://doi.org/10.1007/s10735-013-9482-y

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  • DOI: https://doi.org/10.1007/s10735-013-9482-y

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