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How Myosin 5 Walks Deduced from Single-Molecule Biophysical Approaches

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Myosins

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1239))

Abstract

Myosin 5a is a two-headed myosin that functions as a cargo transporter in cells. To accomplish this task it has evolved several unique structural and kinetic features that allow it to move processively as a single molecule along actin filaments. A plethora of biophysical techniques have been used to elucidate the detailed mechanism of its movement along actin filaments in vitro. This chapter describes how this mechanism was deduced.

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Acknowledgements

JS would like to thank all his many collaborators who participated in studying the molecular mechanism of myosin 5a for their friendship, keen scientific insight and magnificent technical skills. It’s been fun! Particularly, he would like to thank Claudia Veigel, Peter Knight, Justin Molloy, John Trinick (deceased), Stan Burgess, Philipp Kukura, Joanna Andrecka, Howard White, Fei Wang and Takeshi Sakamoto. The authors especially want to thank Peter Knight for his insightful comments on the manuscript and for preparing Fig. 8.16.

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  1. Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    James R. Sellers & Yasuharu Takagi

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  1. James R. Sellers
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  2. Yasuharu Takagi
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Correspondence to James R. Sellers .

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  1. Department of Physiology & Biophysics, Boston University School of Medicine, Boston, MA, USA

    Lynne M. Coluccio

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Sellers, J.R., Takagi, Y. (2020). How Myosin 5 Walks Deduced from Single-Molecule Biophysical Approaches. In: Coluccio, L. (eds) Myosins. Advances in Experimental Medicine and Biology, vol 1239. Springer, Cham. https://doi.org/10.1007/978-3-030-38062-5_8

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