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Developmental and organ-specific changes in DNA-protein interactions in the tomato rbcS1, rbcS2 and rbcS3A promoter regions

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Abstract

DNase I footprinting assays were used to map sites of DNA-protein interaction in the promoter regions of three of the five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase (rbcS) in tomato. Organ-specific differences in DNase I protection patterns were observed using nuclear extracts derived from cotyledons, leaves, young fruit, mature fruit, and roots of tomato, implying that organ-specific transcription of these genes is controlled at the level of DNA-protein interaction. The three genes, designated rbcS1, −2 and −3A are similarly expressed in cotyledons of dark-grown seedlings, in immature tomato fruit, and in leaves under conditions of water stress. These three genes share at least three DNA sequence motifs, including the G-box sequence, which are apparently not present in the other two tomato rbcS genes. We find protection of one or more of these sequences in the aforementioned organs, indicating that the corresponding DNA-binding proteins could function in directing differential expression of the genes, although functional studies would be required to establish this point. While most of the DNase I protections encompass previously identified conserved sequence motifs and their flanking sequences, we also observe protection of additional sequences, many of which occur in the region of the transcription start site.

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Author notes

  1. Thianda Manzara

    Present address: Department of Biological Sciences, University of Illinois at Chicago, 60680, Chicago, IL, USA

  2. Pedro Carrasco

    Present address: Departmento de Bioquimica y Biologia Molecular, Universidad de Valencia, Dr. Moliner 50, E-46100, Burjassot, Valencia, Spain

Authors and Affiliations

  1. Department of Plant Biology, University of California, 94720, Berkeley, CA, USA

    Thianda Manzara, Pedro Carrasco & Wilhelm Gruissem

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  1. Thianda Manzara
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  2. Pedro Carrasco
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  3. Wilhelm Gruissem
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Manzara, T., Carrasco, P. & Gruissem, W. Developmental and organ-specific changes in DNA-protein interactions in the tomato rbcS1, rbcS2 and rbcS3A promoter regions. Plant Mol Biol 21, 69–88 (1993). https://doi.org/10.1007/BF00039619

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  • DOI: https://doi.org/10.1007/BF00039619

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