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Taxonomic implications of Prototheca and Chlorella cell wall polysaccharide characterization

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Summary

Four polysaccharide fractions were obtained by acid and alkaline degradation of purified cell walls of Prototheca spp. and Chlorella spp. These fractions were further hydrolyzed and the component sugars identified. Five Prototheca strains and two Chlorella protothecoides strains have essentially similar polysaccharide compositions, which significantly differ from those of C. vulgaris and C. pyrenoidosa. This emphasizes the close affinity of C. protothecoides to Prototheca spp. not shared by other Chlorella spp.

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References

  • Butler, E. E.: Radiation-induced chlorophyll-less mutants of Chlorella. Science 120, 274–275 (1954).

    Google Scholar 

  • Ciferri, R., Montemartini, A., Ciferri, O.: Caratteristiche morfologiche e assimilative e speciologia della Prototheae. Nuovi Ann. Ig. 8, 554–563 (1957).

    Google Scholar 

  • Cooke, W. B.: Studies in the genus Prototheca. I. Literature review. J. Elisha Mitchell Sci. Soc. 84, 213–216 (1968).

    Google Scholar 

  • Dische, Z., Borenfreund, E.: A spectrophotometric method for the microdetermination of hexosamines. J. biol. Chem. 184, 517–522 (1950).

    Google Scholar 

  • Eaton, N. R.: New press for disruption of microorganisms. J. Bact. 83, 1359–1360 (1962).

    Google Scholar 

  • Elson, L. A., Morgan, W. T. J.: A colorimetric method for the determination of glucosamine and chondrosamine. In: Methods in enzymology, E. F. Neufeld, V. Ginsburg, Eds., Vol. VIII, p. 57. New York: Academic Press 1933.

    Google Scholar 

  • Krüger, W.: Beiträge zur Kenntnis der Organismen des Saftflusses (sog. Schleimflusses) der Laubbäume. Zopf's Beitr. Phys. u. Morph. nied. Org. (Lpzg) 4, 69–116 (1894).

    Google Scholar 

  • LeJohn, H. B.: Enzyme regulation, lysine pathways, and cell wall structures as indicators of major lines of evolution in fungi. Nature (Lond.) 231, 164–168 (1971).

    Google Scholar 

  • Lloyd, D., Turner, G.: The cell wall of Prototheca zopfii. J. gen. Microbiol. 50, 421–427 (1968).

    Google Scholar 

  • Mahadevan, P. R., Tatum, E. L.: Relationship of the major constituents of the Neurospora crassa cell wall to wild type and colonial morphology. J. Bact. 90, 1073–1081 (1965).

    Google Scholar 

  • Nevo, Z., Sharon, N.: The cell wall of Peridinium westii, a noncellulosic glucan. Biochim. biophys. Acta (Amst.) 173, 161–176 (1969).

    Google Scholar 

  • Northcote, D. H., Goulding, K. J., Horne, R. W.: The chemical composition and structure of the cell wall of Chlorella pyrenoidosa. Biochem. J. 70, 381–397 (1958).

    Google Scholar 

  • Parker, B. C.: Significance of cell wall chemistry to phylogeny in the algae. Ann. N.Y. Acad Sci. 175, 417–428 (1970).

    Google Scholar 

  • Pore, R. S.: Nutritional basis for relating Prototheca and Chlorella. Canad. J. Microbiol. 18, 1175–1177 (1972).

    Google Scholar 

  • Pringsheim, E. G.: Farblose Algen. Stuttgart: Fischer 1963.

    Google Scholar 

  • Seifter, S., Dayton, S., Novic, B., Muntwyler, E.: The estimation of glycogen with anthrone reagent. Arch. Biochem. 25, 191–200 (1950).

    Google Scholar 

  • Shihira, I., Krauss, R. W.: Chlorella: Physiology and taxonomy of fourty-one isolates. Baltimore: Port City Press 1965.

    Google Scholar 

  • Turner, G., Lloyd, D.: The chemical composition and structure of the cell wall of Prototheca zopfii. Biochem. J. 99, 55–56 (1966).

    Google Scholar 

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Conte, M.V., Pore, R.S. Taxonomic implications of Prototheca and Chlorella cell wall polysaccharide characterization. Archiv. Mikrobiol. 92, 227–233 (1973). https://doi.org/10.1007/BF00411203

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  • DOI: https://doi.org/10.1007/BF00411203

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