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Methane production potential and methanogenic archaea community dynamics along the Spartina alterniflora invasion chronosequence in a coastal salt marsh

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Abstract

Invasion by the exotic species Spartina alterniflora, which has high net primary productivity and superior reproductive capacity compared with native plants, has led to rapid organic carbon accumulation and increased methane (CH4) emission in the coastal salt marsh of China. To elucidate the mechanisms underlying this effect, the methanogen community structure and CH4 production potential as well as soil organic carbon (SOC), dissolved organic carbon, dissolved organic acids, methylated amines, aboveground biomass, and litter mass were measured during the invasion chronosequence (0–16 years). The CH4 production potential in the S. alterniflora marsh (range, 2.94–3.95 μg kg−1 day−1) was significantly higher than that in the bare tidal mudflat. CH4 production potential correlated significantly with SOC, acetate, and trimethylamine concentrations in the 0–20 cm soil layer. The abundance of methanogenic archaea also correlated significantly with SOC, and the dominant species clearly varied with S. alterniflora-driven SOC accumulation. The acetotrophic Methanosaetaceae family members comprised a substantial proportion of the methanogenic archaea in the bare tidal mudflat while Methanosarcinaceae family members utilized methylated amines as substrates in the S. alterniflora marsh. Ordination analysis indicated that trimethylamine concentration was the primary factor inducing the shift in the methanogenic archaea composition, and regressive analysis indicated that the facultative family Methanosarcinaceae increased linearly with trimethylamine concentration in the increasingly sulfate-rich salt marsh. Our results indicate that increased CH4 production during the S. alterniflora invasion chronosequence was due to increased levels of the non-competitive substrate trimethylamine and a shift in the methanogenic archaea community.

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Acknowledgment

This work was supported by the strategic priority research program—Climate Change: Carbon Budget and Relevant Issues of the Chinese Academy of Sciences (XDA05020501), the National Basic Research Program of China (2012CB417102), and the Natural Science Foundation of China (41171190, 41001045, and 41001173).

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  1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Junji Yuan, Weixin Ding, Deyan Liu, Jian Xiang & Yongxin Lin

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Junji Yuan, Jian Xiang & Yongxin Lin

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  1. Junji Yuan
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  2. Weixin Ding
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  3. Deyan Liu
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Yuan, J., Ding, W., Liu, D. et al. Methane production potential and methanogenic archaea community dynamics along the Spartina alterniflora invasion chronosequence in a coastal salt marsh. Appl Microbiol Biotechnol 98, 1817–1829 (2014). https://doi.org/10.1007/s00253-013-5104-6

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