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High Level of Genetic Diversity Among the Selected Accessions of Tea (Camellia sinensis) from Abandoned Tea Gardens in Western Himalaya

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Abstract

To revive cultivation of the tea unique to the western Himalayan region, it is important to evaluate the seed-derived bushes available in the area’s abandoned gardens. This study used quantitative leaf characters, catechin content, and AFLP markers to assess these China cultivar type bushes. Compared with other China cultivar germplasm, these accessions showed a higher level of diversity among themselves. Among the quantitative morphological characters, leaf length is important in distinguishing the accessions studied, with a high loading value in the principal component analysis. The catechins and AFLP markers displayed the genetic makeup of the accessions. Other than total catechins, the trihydroxylated catechins showed a high loading value in differentiating the accessions. The genetic control of the ratio of dihydroxylated and trihydroxylated catechins is found to be based on a correlation with AFLP markers. The genetic similarity between Kangra Asha and Kangra Jat suggests that Kangra Jat must be descended from Kangra Asha. Kangra Jat is well adapted to local environmental conditions, as is evident from its high catechin content.

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References

  • Balasaravanan T, Pius PK, Raj Kumar R, Muraleedharan N, Shasany AK (2003) Genetic diversity among the south Indian germplasm (Camellia sinensis, C. assamica spp. lasiocalyx) using AFLP markers. Plant Sci 165:365–372

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:1–15

    Google Scholar 

  • Evans JC (1992) Tea in China. Greenwood Press, Westport, pp 1–22

    Google Scholar 

  • Gerats AM, Martin C (1992) Flavanoid synthesis in Petunia hybrida: genetics and molecular biology of flower colour. In: Stafford HA, Ibrahim RK (eds) Phenolic metabolism in plants. Plenum Press, New York, pp 167–175

    Google Scholar 

  • Hammer O, Harper DAT, Ryan PD (2001) PAST: paleaobiological statistics software package for education and data analysis. Palaeontol Electronica 4(1): 9pp

  • IPGRI (1997) Descriptors for tea (Camellia sinensis). International Plant Genetic Resources Institute, Rome

    Google Scholar 

  • Kaundun SS, Park YG (2002) Genetic structure of six Korean tea populations as revealed by RAPD-PCR markers. Crop Sci 42:594–601

    CAS  Google Scholar 

  • Kaundun SS, Zhyvoloup A, Park YG (2000) Evaluation of the genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers. Euphytica 115:7–16

    Article  CAS  Google Scholar 

  • Lai JA, Yang WC, Hsiao JY (2001) An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR maskers. Bot Bull Acad Sinica 42:93–100

    CAS  Google Scholar 

  • Lee SH, Choi HS, Kim RS, Lee HY, Nou IS (1995) Identification of Korean wild tea plants and Japanese green tea cultivars using RAPD markers. J Korean Tea Sci 1:129–148

    Google Scholar 

  • Li HL (1983) The domestication of plants in China: ecogeographical considerations. In: Keightley DN (ed) The origins of Chinese civilization. University of California Press, Berkeley and Los Angeles

    Google Scholar 

  • Magoma GN, Wachira FN, Obanda M, Imbuga M, Agong SG (2000) The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genet Resour Crop Evol 47:107–114

    Article  Google Scholar 

  • Matsumoto S, Takeuchi A, Hayatsu M, Kondo S (1994) Molecular cloning of phenylalanine ammonia lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theor Appl Genet 89:671–675

    Article  CAS  Google Scholar 

  • Muraleedharan N (1999) Tea research in South India. Planters Chron 95(10):481–483

    Google Scholar 

  • Obanda M, Owuor P (1997) Flavonols composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric 74:209–215

    Article  CAS  Google Scholar 

  • Paul S, Wachira FN, Powell W, Waugh R (1997) Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor Appl Genet 94:255–263

    Article  CAS  Google Scholar 

  • Saravana M, Maria John KM, Rajkumar R, Pius PK, Sasikumar R (2005) Genetic diversity of UPASI tea clones (Camellia sinensis (L.) O. Kuntze). On the basis of total catechins and their fractions. Phytochemistry 66(2005):561–565

    Article  Google Scholar 

  • Sharma V, Gulati A, Ravindranath SD, Kumar V (2005) A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. J Food Compost Anal 18(6):583–594

    Article  CAS  Google Scholar 

  • Ukers WH (1935) All about tea. Tea and Coffee Trade Journal Company, New York

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van Der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Wachira F, Wauch R, Hachett CA, Powell W (1995) Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38:201–210

    Article  PubMed  CAS  Google Scholar 

  • Wachira FN, Powell W, Waugh R (1997) An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle specific STS. Heredity 78:603–611

    Article  CAS  Google Scholar 

  • Wachira F, Tanaka J, Takeda Y (2001) Genetic variation and differentiation in tea (Camellia sinensis) germplasm revealed by RAPD and AFLP variation. J Hortic Sci Biotechnol 76:557–563

    CAS  Google Scholar 

  • Weatherstone J (1992) Historical introduction. In: Willson KC, Clifford MN (eds) Tea: cultivation to consumption. Chapman and Hall, London

    Google Scholar 

Download references

Acknowledgments

We are grateful for financial assistance from the Council of Scientific and Industrial Research, New Delhi, under the NMITLI program and the Department of Biotechnology (Govt. of India).

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Correspondence to S. Rajkumar.

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Karthigeyan, S., Rajkumar, S., Sharma, R.K. et al. High Level of Genetic Diversity Among the Selected Accessions of Tea (Camellia sinensis) from Abandoned Tea Gardens in Western Himalaya. Biochem Genet 46, 810–819 (2008). https://doi.org/10.1007/s10528-008-9195-1

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  • DOI: https://doi.org/10.1007/s10528-008-9195-1

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