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Cloning and molecular characterization of a myosin light chain gene from Puccinia striiformis f. sp. tritici

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Abstract

The fungus Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, is an obligate biotrophic basidiomycete. Many studies have found that myosins play important roles during fungal growth and propagation. However, there are few reports on the myosins of Pst. In this study, we cloned and obtained the myosin light chain gene PsMLC1 from Pst and characterized its expression. Furthermore, the function of PsMLC1 was identified by mutant complementation. As a result, we found that expression of PsMLC1 in Schizosaccharomyces pombe mostly complemented the defects of the cdc4 mutant, indicating that PsMLC1 belongs to the myosin light chain family member. Expression studies showed that the transcript levels of PsMLC1 little changed before 24 h post inoculation then was suddenly down-regulated during Pst infection of wheat. By using ML-7, we observed that inactivity of PsMLC1 greatly reduced the germination rate of urediniospores. These results suggest that PsMLC1 is essential for the early stages of Pst infection of wheat but unnecessary for the later stages of infection. This work elucidates the function of the myosins in Pst and may provide some theoretical basis for controlling strip rust.

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Acknowledgments

This study was financially supported by National Key Basic Research Program of China (2013CB127700), the National Natural Science Foundation of China (No. 31000078), and the 111 Project from the Ministry of Education of China (B07049). We thank Dr. Dannel McCollum for S. pombe cdc4 mutant and the vector pREP41.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Jie Liu, Li-Na Han, Qiong Zhang, Qiu-Ling Wang, Jia Liu & Man Li

  2. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Qing Chang, Hua Zhuang, Dan Yu & Zhen-Sheng Kang

Authors
  1. Jie Liu
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  2. Li-Na Han
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  3. Qiong Zhang
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  4. Qiu-Ling Wang
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  5. Qing Chang
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  6. Hua Zhuang
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  7. Jia Liu
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  8. Man Li
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  9. Dan Yu
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  10. Zhen-Sheng Kang
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Correspondence to Zhen-Sheng Kang.

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Jie Liu and Lina Han have contributed equally to this study.

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Liu, J., Han, LN., Zhang, Q. et al. Cloning and molecular characterization of a myosin light chain gene from Puccinia striiformis f. sp. tritici . World J Microbiol Biotechnol 30, 631–637 (2014). https://doi.org/10.1007/s11274-013-1485-z

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  • DOI: https://doi.org/10.1007/s11274-013-1485-z

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