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Adipose Tissue Distribution and Quantification of PPARβ/δ and PPARγ1-3 mRNAs: Discordant Gene Expression in Subcutaneous, Retroperitoneal and Visceral Adipose Tissue of Morbidly Obese Patients

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Background

Adipose tissue (AT) metabolism is altered in obese subjects, and the reestablishment of energy homeostasis requires the identification and regulation of genes with altered patterns. The aim of this study was to compare mRNA expression of PPARβ/δ and PPARγ1-3 in morbidly obese and nonobese patients. The expression pattern of these receptors in various abdominal adipose tissues, subcutaneous (SAT), retroperitoneal (RAT) and visceral (VAT), was also evaluated.

Methods

The AT depots were obtained by surgery. Total RNAs were extracted using TRIzol. PPARs reverse transcripts were determined by quantitative polymerase chain reaction (qRT-PCR).

Results

The amounts of PPARβ/δ mRNA in different depots of morbidly obese AT showed a significant decrease in VAT (P < 0.05). In the non-obese group, the level of PPARβ/δ was higher in SAT (P < 0.05), but PPARγ1-3 was not differentially expressed in obese and non-obese depots. When comparing obese and non-obese, the results revealed a decrease in PPARβ/δ expression in SAT (P = 0.058) and VAT (P = 0.094) of the morbidly obese. PPARγ1-3 mRNA expression was increased significantly in SAT (P = 0.022) and decreased in RAT (P = 0.034) in morbidly obese subjects. PPARβ/δ expression in SAT and VAT correlated negatively with hip size and insulin serum respectively. PPARγ1-3 expression in RAT correlated negatively with waist and hip circumference and in VAT correlated positively with waist size.

Conclusions

The present study demonstrates that PPARβ/δ and PPARγ1-3 mRNAs are quantitatively different in AT of morbidly obese individuals compared to non-obese, and that PPARβ/δ mRNA levels are characteristic for each AT depot.

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Authors and Affiliations

  1. Departamento de Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos 2600 – anexo, CEP 90.035-003, Porto Alegre, RS, Brazil

    Josiane Woutheres Bortolotto, Rogério Margis PhD, Ângela Cristine Bersch Ferreira & Regina Maria Guaragna PhD

  2. Centro de Obesidade Mórbida, PUCRS, Porto Alegre, Brazil

    Alexandre Vontobel Padoin MD & Cláudio Cora Mottin PhD

Authors
  1. Josiane Woutheres Bortolotto
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  2. Rogério Margis PhD
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  3. Ângela Cristine Bersch Ferreira
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  4. Alexandre Vontobel Padoin MD
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  5. Cláudio Cora Mottin PhD
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  6. Regina Maria Guaragna PhD
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Correspondence to Regina Maria Guaragna PhD.

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Bortolotto, J.W., Margis, R., Ferreira, Â.C.B. et al. Adipose Tissue Distribution and Quantification of PPARβ/δ and PPARγ1-3 mRNAs: Discordant Gene Expression in Subcutaneous, Retroperitoneal and Visceral Adipose Tissue of Morbidly Obese Patients. OBES SURG 17, 934–940 (2007). https://doi.org/10.1007/s11695-007-9172-5

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  • DOI: https://doi.org/10.1007/s11695-007-9172-5

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