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Circulating Concentration of Chemical Elements During Exercise-Induced Muscle Damage and the Repeated Bout Effect

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Abstract

The objective of the study was to evaluate the circulating levels of chemical elements after exercise-induced muscle damage (EIMD) followed by the repeated bout effect (RBE). Seven physically active subjects (26.5 ± 4.0 years) performed two sessions of EIMD (5 sets of 20 drop jumps), the second session 14 days after the first for RBE assessment. Blood collections, countermovement jump (CMJ), squat jump (SJ), and delayed-onset muscle soreness (DOMS) were performed before (Pre), after (Post), and 24, 48, and 72 h after the exercise session. Creatine kinase (CK) was detected by biochemical analysis and the concentration of chemical elements by total reflection X-ray fluorescence (TXRF). Differences between time points and sessions were assessed with two-way ANOVA and the effect size (ES). EIMD induced a reduction in the CMJ at 24 h (P < 0.05) and an increase in DOMS at 24 h (P < 0.01) and 48 h (P < 0.01), and CK at 72 h (P < 0.05). RBE alleviated all symptoms of EIMD in the second session (P > 0.05). EIMD induced a large to very large ES for Zn reduction at 24 h (− 1.37) and 72 h (− 0.93) and Br (− 0.83) at 72 h. RBE presented large to very large ES for the increase in P at 48 h (0.92); Cl at 24 h (1.04); K at 24 h (0.91), 48 h (1.10), and 72 h (0.96); Ca at 72 h (0.92); and Fe at 24 h (0.85). RBE influenced the concentration of elements associated with fatigue (K, Ca, Cl), inflammatory response, and glucose metabolism (Zn).

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Data Availability

The authors declare that study data are available from the corresponding author at readers’ request.

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Acknowledgements

The authors would like to thank the Edge academy (Londrina-PR) for allowing the use of their facilities for evaluation and data collection.

Funding

The study had financial support from Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil) for the Master’s scholarship to SP (grant n. 1798841/2018).

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Authors and Affiliations

  1. Associate Postgraduate Program in Physical Education UEL-UEM, State University of Londrina, Londrina, Brazil

    Silas Seolin Dias, Martim Gomes Weber & Susana Padoin

  2. Department of Physics, State University of Londrina, Londrina, Brazil

    Avacir Casanova Andrello & Eduardo Inocente Jussiani

  3. Study Groups on Tissue Regeneration, Adaptation, and Repair, State University of Londrina, Londrina, Brazil

    Solange de Paula Ramos

  4. Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid PR 445 Km 380, Londrina, PR, CEP: 86099-100, Brazil

    Solange de Paula Ramos

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  1. Silas Seolin Dias
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Contributions

S.S. Dias: conceptualization, methodology, validation, formal analysis, investigation, resources, data curation, writing the original draft.

M.G. Weber: methodology, investigation, data curation, writing the original draft.

S. Padoin: conceptualization, methodology, investigation, resources.

A.C. Andrello: conceptualization, methodology, validation, formal analysis, investigation, data curation, writing the original draft.

E.I. Jussiani: conceptualization, methodology, validation, formal analysis, investigation, resources, data curation, writing the original draft.

S.de Paula Ramos: supervision, project administration, resources, writing the original data.

Corresponding author

Correspondence to Solange de Paula Ramos.

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Dias, S.S., Weber, M.G., Padoin, S. et al. Circulating Concentration of Chemical Elements During Exercise-Induced Muscle Damage and the Repeated Bout Effect. Biol Trace Elem Res 200, 1060–1070 (2022). https://doi.org/10.1007/s12011-021-02737-8

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