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Species-specific expression variation of fish MYH14, an ancient vertebrate myosin heavy chain gene orthologue

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  • Chemistry and Biochemistry
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Abstract

MYH14 (MYH7b) is the most recently identified sarcomeric myosin heavy chain gene (MYH) from the human genome. Recent observations have revealed that MYH M5 , a torafugu orthologue of MYH14, is one of the major components of the MYH repertoire expressed in torafugu embryos and adults, suggesting its pivotal role in fish muscle formation. We have examined the expression pattern of MYH14 in zebrafish, a model organism for the study of vertebrate development. In situ hybridization studies revealed that zebrafish MYH14 was expressed in the most myotomal region containing fast muscle fibers in the embryonic stage and in superficial slow muscle fibers in the adult stage. Weak signals of MYH14 transcripts were also detected in intermediate muscle fibers located between superficial slow and inner fast muscle fibers in the adult. Reverse transcription-PCR studies showed that MYH14 was not only expressed in skeletal muscles but also in cardiac muscles. These expression patterns are in a marked contrast to that of torafugu MYH M5 , which is solely expressed in slow and cardiac muscle fibers in both the embryonic and adult stages. Our results imply species-specific functions of MYH14 in fish muscle formation.

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Acknowledgments

This study was partly supported by a Grant-in-Aid for Scientific Research from the Japan Society for Promotion of Science.

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Authors and Affiliations

  1. Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan

    Shigeharu Kinoshita, Bhuiyan Sharmin Siddique, Md. Asaduzzaman, Shuichi Asakawa & Shugo Watabe

  2. Hınıs Vocational Training School and Biotechnology Application and Research Center, Atatürk University, 25240, Erzurum, Turkey

    Saltuk Bugrahan Ceyhun

Authors
  1. Shigeharu Kinoshita
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  2. Bhuiyan Sharmin Siddique
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  3. Saltuk Bugrahan Ceyhun
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  4. Md. Asaduzzaman
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  5. Shuichi Asakawa
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  6. Shugo Watabe
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Correspondence to Shugo Watabe.

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Kinoshita, S., Siddique, B.S., Ceyhun, S.B. et al. Species-specific expression variation of fish MYH14, an ancient vertebrate myosin heavy chain gene orthologue. Fish Sci 77, 847–853 (2011). https://doi.org/10.1007/s12562-011-0375-2

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  • DOI: https://doi.org/10.1007/s12562-011-0375-2

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