Single Residue Acts as Gate in OccK Channels
- Karunakar R. Pothula
- ,
- Naresh N. Dhanasekar
- ,
- Usha Lamichhane
- ,
- Farhan Younas
- ,
- Daniel Pletzer
- ,
- Roland Benz
- ,
- Mathias Winterhalter
- , and
- Ulrich Kleinekathöfer
Abstract
The OccK protein subfamily located in the outer membrane of Pseudomonas aeruginosa contains dynamic channels with several conformational states that range from open to closed forms. The molecular determinants of the OccK channels that contribute to the diverse gating have, however, remained elusive so far. Performing molecular dynamics (MD) simulations on OccK5 (OpdH) as an example, local fluctuations of loop L7 mediated by a single residue were identified that effectively gate the channel. The features of this gate residue were studied by single-channel electrophysiology and site-directed mutagenesis demonstrating that this gate residue indeed confers unique gating properties to the OccK channels. In support of these functional measurements, MD simulations highlight the correlations between the size of the side-chain belonging to the gate residue on one side and the pore size as well as the L7 flexibility on the other side.
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