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Quantifying the Diffusion of Lipids in the Proximal/Distal Leaflets of a Supported Lipid Bilayer by Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy

Cite this: J. Phys. Chem. B 2018, 122, 45, 10315–10319
Publication Date (Web):October 26, 2018
https://doi.org/10.1021/acs.jpcb.8b08614
Copyright © 2018 American Chemical Society
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    Abstract

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    A supported lipid bilayer (SLB) is a versatile platform for examining the dynamical properties of biomembranes. However, the effect of a prerequisite solid substrate on the dynamics of a SLB remains very elusive. Especially, it is not clarified how the diffusivity of each leaflet in a SLB is affected by the SLB–solid substrate interaction. In this study, we applied two-dimensional fluorescence lifetime correlation spectroscopy to a SLB for elucidating the diffusion of lipids in the proximal and distal leaflets of a SLB. We find that the autocorrelation curve of a fluorescent lipid in the proximal leaflet decays more slowly than that in the distal leaflet, meaning that the proximal leaflet is less diffusive. This result indicates stronger interaction between the proximal leaflet and a solid substrate.

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    Cited By

    This article is cited by 13 publications.

    1. Ikumi Mori, Shun Terasaka, Shoichi Yamaguchi, Takuhiro Otosu. Diffusion of Multiple Species Resolved by Fluorescence Lifetime Recovery after Photobleaching (FLRAP). Analytical Chemistry 2024, 96 (12) , 4854-4859. https://doi.org/10.1021/acs.analchem.3c05181
    2. Rodrigo Noriega. Measuring the Multiscale Dynamics, Structure, and Function of Biomolecules at Interfaces. The Journal of Physical Chemistry B 2021, 125 (22) , 5667-5675. https://doi.org/10.1021/acs.jpcb.1c01546
    3. Bidyut Sarkar, Kunihiko Ishii, Tahei Tahara. Microsecond Folding of preQ1 Riboswitch and Its Biological Significance Revealed by Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy. Journal of the American Chemical Society 2021, 143 (21) , 7968-7978. https://doi.org/10.1021/jacs.1c01077
    4. Kosei Shimizu, Miyuki Sakaguchi, Shoichi Yamaguchi, Takuhiro Otosu. Peripheral adsorption of polylysine on one leaflet of a lipid bilayer reduces the lipid diffusion of both leaflets. Physical Chemistry Chemical Physics 2024, 26 (11) , 8873-8878. https://doi.org/10.1039/D3CP04882A
    5. Stefan L Schaefer, Gerhard Hummer. Sublytic gasdermin-D pores captured in atomistic molecular simulations. eLife 2022, 11 https://doi.org/10.7554/eLife.81432
    6. Subhrajit Mohanty, Usharani Subuddhi. Fluorescence Lifetime: A Multifaceted Tool for Exploring Biological Systems. 2022, 77-111. https://doi.org/10.1007/978-981-16-4550-1_5
    7. K.G. Schaefer, A.E. Pittman, F.N. Barrera, G.M. King. Atomic force microscopy for quantitative understanding of peptide-induced lipid bilayer remodeling. Methods 2022, 197 , 20-29. https://doi.org/10.1016/j.ymeth.2020.10.014
    8. Rafael L. Schoch, Frank L. H. Brown, Gilad Haran. Correlated diffusion in lipid bilayers. Proceedings of the National Academy of Sciences 2021, 118 (48) https://doi.org/10.1073/pnas.2113202118
    9. Arindam Ghosh, Jörg Enderlein. Advanced fluorescence correlation spectroscopy for studying biomolecular conformation. Current Opinion in Structural Biology 2021, 70 , 123-131. https://doi.org/10.1016/j.sbi.2021.06.018
    10. N.V. Surovtsev, S.V. Adichtchev. Low‐wavenumber Raman scattering of phospholipid bilayers in fluid, ripple, and gel phases. Journal of Raman Spectroscopy 2020, 51 (6) , 952-958. https://doi.org/10.1002/jrs.5861
    11. Takuhiro Otosu, Shoichi Yamaguchi. Effect of electrostatic interaction on the leaflet-specific diffusion in a supported lipid bilayer revealed by fluorescence lifetime correlation analysis. Physical Chemistry Chemical Physics 2020, 22 (3) , 1242-1249. https://doi.org/10.1039/C9CP05833H
    12. Takuhiro Otosu, Shoichi Yamaguchi. Reduction of glass-surface charge density slows the lipid diffusion in the proximal leaflet of a supported lipid bilayer. The Journal of Chemical Physics 2019, 151 (2) https://doi.org/10.1063/1.5103221
    13. Takuhiro Otosu, Shoichi Yamaguchi. Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications. Molecules 2018, 23 (11) , 2972. https://doi.org/10.3390/molecules23112972
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