Modulation of the Activity of the Insulin-Degrading Enzyme by Aβ Peptides

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acschemneuro.3c00384.

• Steady-state kinetic parameters for the degradation of insulin by IDE in the absence and presence of Aβ peptides determined from Michaelis–Menten and Lineweaver–Burk plots, MALDI-TOF mass spectra of IDE-dependent degradation of insulin and corresponding table of observed and theoretical masses of insulin fragments, cleavage sites in insulin identified by MALDI-TOF mass spectrometry, MALDI-TOF mass spectra of IDE-dependent degradation of Aβ(1–40) and corresponding table of observed and theoretical masses of Aβ(1–40) fragments, MALDI-TOF mass spectra of IDE-dependent degradation of Aβ(pyroE3–42), circular dichroic spectra of insulin and insulin + Aβ samples in the absence of IDE, MALDI-TOF mass spectra of IDE-dependent degradation of insulin in the presence of Aβ(1–40) and Aβ(pyroE3–42) and corresponding table of observed and theoretical masses of insulin and Aβ(1–40) fragments, and MALDI-TOF mass spectra of IDE-dependent degradation of Aβ(1–40) in the presence of Aβ(pyroE3–42) (PDF)