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Plants as Biofactories: Physiological Role of Reactive Oxygen Species on the Accumulation of Phenolic Antioxidants in Carrot Tissue under Wounding and Hyperoxia Stress

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Department of Horticultural Sciences, Texas A&M University, Vegetable & Fruit Improvement Center, College Station, Texas 77843-2133, United States
Postharvest and Refrigeration Group, Department of Food Engineering, Technical University of Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena, Murcia, Spain
§ Instituto de Ciencia y Tecnología de Alimentos, Facultad de Agronomía y Agroindustrias, Universidad Nacional de Santiago del Estero, 4200 Santiago del Estero, Argentina
Tel: +979-845-3244. Fax: +979-845-0627. E-mail: [email protected]
Cite this: J. Agric. Food Chem. 2011, 59, 12, 6583–6593
Publication Date (Web):May 9, 2011
https://doi.org/10.1021/jf2006529
Copyright © 2011 American Chemical Society

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    Abstract

    Plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. Here, we report the potential use of carrots (Daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. Wounding stress induced an increase of ∼287% in total phenolic content (PC) in carrots stored for 48 h at 20 °C. This increase was higher (∼349%) in the wounded tissue treated with hyperoxia stress. To further understand the physiological role of reactive oxygen species (ROS) as a signaling molecule for the stress-induced accumulation of phenolics in carrots, the respiration rate as well as the enzymatic activities of NADPH oxidase, superoxide dismutase, ascorbate peroxidase, and catalase were evaluated. Likewise, shredded carrots were treated with diphenyleneiodonium chloride solution to block NADPH oxidase ROS productions, and the phenylalanine ammonia lyase activity and total PC were evaluated. Results demonstrated that ROS play a key role as a signaling molecule for the stress-induced accumulation of PC in carrots.

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