Abstract
With the use of molecular ecology methods, the archaeal component of microbial mats on coral-like structures associated with methane seeps occurring at a depth of about 200 m in the Black Sea was investigated without the isolation of pure cultures. Using archaea-specific 16S rDNA–targeted oligonucleotide primes, long fragments of genes were amplified, cloned, and sequenced and their phylogenetic analysis was carried out. It was shown that archaea in microbial mats on coral-like structures are represented by two dominant phylotypes that belong to the kingdoms Crenarchaeota and Euryarchaeota and are not specifically related to any described archaeal species. The possible role of the revealed archaea in the process of anaerobic methane oxidation is discussed.
Similar content being viewed by others
REFERENCES
Zehnder, A.J. and Brock, T.D., Anaerobic Methane Oxidation: Occurrence and Ecology, Appl. Environ. Microbiol., 1980, vol. 39, pp. 194-204.
Hoehler, T.M., Alperin, M.J., Albert, D.B., and Martens, C.S., Field and Laboratory Studies of Methane Oxidation in Anoxic Marine Sediments: Evidence for a Methanogen-Sulfate Reducer Consortium, Global Biogeochem. Cycles, 1994, vol. 8, pp. 451-463.
Hinrichs, K.-U., Hayes, J.M., Sylva, S.P., Brewer, P.G., and DeLong, E.F., Methane-Consuming Archaebacteria in Marine Sediments, Nature(London), 1999, vol. 398, pp. 802-805.
Boetius, A., Ravenschlag, K., Schubert, G.J., Rickert, D., Widdel, F., Gieseke, A., Amann, R., Jorgensen, B.B., Witte, U., and Pfannkuche, O., A Marine Microbial Consortium Apparently Mediating Anaerobic Oxidation of Methane, Nature(London), 2000, vol. 407, pp. 623-626.
Orphan, V.J. and Hinrichs, K.-U., Ussler III, W., Paull, C.K., Taylor, L.T., Sylva, S.P., Hayes, J.M., and DeLong, E.F., Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments, Appl. Environ. Microbiol.,2001, vol. 67, pp. 1922-1934.
Orphan, V.J., House, C.H., Hinrichs, K.-U., McKeegan, K.D., and DeLong, E.F., Methane-Consuming Archaea Revealed by Directly Coupled Isotopic and Phylogenetic Analyses, Science,2001, vol. 293, pp. 484-487.
Salentine, D.L. and Reeburgh, W.S., New Perspectives on Anaerobic Methane Oxidation, Environ. Microbiol., 2000, vol. 2, pp. 477-484.
Ivanov, M.V., Polikarpov, G.G., Lein, A.Yu., et al., Biogeochemistry of the Carbon Cycle in the Region of Methane Seeps in the Black Sea, Dokl. Akad. Nauk SSSR, 1991, vol. 320, no. 5, pp. 1235-1240.
Pimenov, N.V., Rusanov, I.I., Poglazova, M.N., et al., Bacterial Mats on Coral-like Structures at Methane Seeps in the Black Sea, Mikrobiologiya, 1997, vol. 66, no. 3, pp. 412-428.
Kolganova, T.V., Kuznetsov, B.B., and Tourova, T.P., Designing and Testing Oligonucleotide Primers for Amplification and Sequencing of Archaeal 16S rRNA Genes, Mikrobiologiya, 2002, vol. 71, no. 2, pp. 283-286.
Edwards, U., Rogall, T., Bloeker, H., Ende, M.D., and Boeettge, E.C., Isolation and Direct Complete Nucleotide Determination of Entire Genes, Characterization of Gene Coding for 16S Ribosomal RNA, Nucleic Acids Res., 1989, vol. 17, pp. 7843-7853.
Holmes, A.J., Costello, A., Lidstrom, M.E., and Murrel, J.C., Evidence That Particulate Methane Monooxygenase and Ammonia Monooxygenase May Be Evolutionary Related, FEMS Microbiol. Lett., 1995, vol. 132, pp. 203-208.
Felsenstein, J., PHYLIP, Phylogenetic Inference Package (Version 3.2), Cladistics, 1989, vol. 5, pp. 164-166.
Vetriani, C., Jannasch, H.W., MacGregor, B., Stahl, D.A., and Reysenbach, A.-L., Population Structure and Phylogenetic Characterization of Marine Benthic Archaea in Deep-Sea Sediments, Appl. Environ. Microbiol., 1999, vol. 65, no. 10, pp. 4375-4384.
Thomsen, T.R., Finster, K., and Ramsing, N.B., Biogeochemical and Molecular Signatures of Anaerobic Methane Oxidation in a Marine Sediment, Appl. Environ. Microbiol., 2001, vol. 67, pp. 1646-1656.
Takai, K. and Horikoshi, K., Genetic Diversity of Archaea in Deep-Sea Hydrothermal Vent Environments, Genetics,1999, vol. 152, pp. 1285-1297.
Schleper, C., DeLong, E.F., Preston, C.M., Feldman, F.A., Wu, K.-Y., and Swanson, R.V., Genomic Analysis Reveals Chromosomal Variation in Natural Populations of the Uncultured Psychrophilic Archaeon Cenarchaeum symbiosum, J. Bacteriol., 1998, vol. 180, pp. 5003-5009.
Preston, C.M., Wu, K.Y., Molinski, T.F., and DeLong, E.F., A Psychrophilic Crenarchaeon Inhabits a Marine Sponge: Cenarchaeum symbiosumgen. nov., sp. nov, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 6241-6246.
Christoserdova, L., Vorholt, J.A., Thauer, R.K., and Lidstrom, M.E., C1 Transfer Enzymes and Coenzymes Linking Methylotrophic Bacteria and Methanotrophic Archaea, Science, 1998, vol. 281, pp. 99-102.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tourova, T.P., Kolganova, T.V., Kuznetsov, B.B. et al. Phylogenetic Diversity of the Archaeal Component in Microbial Mats on Coral-like Structures Associated with Methane Seeps in the Black Sea. Microbiology 71, 196–201 (2002). https://doi.org/10.1023/A:1015102422144
Issue Date:
DOI: https://doi.org/10.1023/A:1015102422144