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Replication-defective adenovirus type 5 as an in vitro and in vivo gene transfer vector in chickens
- Micheline Adam1, Wahiba Oualikene1, Hervé Le Cocq2, Michèle Guittet2 and Marc Eloit1
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View Affiliations Hide AffiliationsAffiliations: 1 Unité de Génétique Moléculaire, Génétique Virale, INRA, Ecole Nationale Vétérinaire d’Alfort, 94704 Maisons Alfort 2 CNEVA, Laboratoire Central de Recherches Avicole et Porcine, Unité de Pathologie Aviaire, BP 53, 22440 Ploufragan, France
*Author for correspondence. Fax +33 143 96 71 31. e-mail [email protected]
- Published: 01 December 1995 https://doi.org/10.1099/0022-1317-76-12-3153
Abstract
The capacity of E1A gene-deleted and thus replication-defective adenovirus type 5 (Ad5) to transduce foreign genes in chicken embryo fibroblasts (CEF) as well as in chickens was investigated. The lacZ and luciferase genes were successfully transduced in CEF by replication-defective Ad5, demonstrating that these cells possess receptor(s) for binding and penetration of Ad5. A single intramuscular inoculation of Ad-gD, a replication-defective Ad5 harbouring the gD gene of pseudorabies virus, in adult and 1-day-old chickens led to the production of very high titres of specific antibodies. These gD-specific antibodies persisted for at least 56 days. These results demonstrate that replication-defective Ad5, despite its mammalian origin and the deletion of the E1A gene, is a good candidate for developing non-spreading vaccines in poultry.
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References
- Adam M., Le Pottier M. F., Eloit M. 1994; Vaccination of pigs with replication defective adenovirus vectored vaccine: the example of pseudorabies. Veterinary Microbiology 42:205–215
- Ali M., Lemoine N. R., A C. J. 1994; The use of DNA viruses as vectors for gene therapy. Gene Therapy 1:367–384
- Ballay A., Levrero M., Tiollais P., Perricaudet M. 1987; An hepatitis B-adenovirus recombinant as a potential live vaccine. In Hepadnaviruses – U.C.L.A. Symposia on Molecular and Cellular Biology. New Series vol 70481–493 Edited by Robinson W., Koike K., Will H. New York: Alan R. Liss;
- Beard C. W. 1970; Avian influenza antibody detection by immunodiffusion. Avian diseases 14:337–341
- Boursnell M. E. G., Green P. F., Samson A. C. R., Campbell J. I. A., Deuter A., Peters R. W., Millar N. S., Emmerson P. T., Bins M. M. 1990; A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge by NDV. Virology 178:297–300
- Chambers T., Kawaoka Y., Webster R. 1988; Protection of chickens from lethal influenza infection by vaccine-expressed hemagglutinin. Virology 167:414–421
- Cooten M., Wagner K., Zatloukal K., Birnstiel M. L. 1993; Chicken adenovirus (CELO virus) particles augment receptor– mediated DNA delivery to mammalian cells and yield exceptional levels of stable transformants. Journal of Virology 64:3777–3785
- Defer C., Belin M. T., Caillet Boudin M. L., Boulanger P. 1990; Human adenovirus-host cell interactions: comparative study with members of subgroups B and C. Journal of Virology 64:3661–3673
- Eloit M., Gilardi-Hebenstreit P., Toma B., Perricaudet M. 1990; Construction of a defective adenovirus vector expressing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine. Journal of General Virology 71:2425–2431
- Gonin P., Fournier A., Oualikene W., Moraillon A., Eloit M. 1995; Immunization trial of cats with a replication-defective adenovirus type 5 expressing the env gene of feline immunodeficiency virus. Veterinary Microbiology (in press)
- Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
- Guo P., Scholz E., Maloney B., Welniak E. 1994; Construction of recombinant avian infectious laryngotracheitis virus expressing the β-galactosidase gene and DNA sequencing of the insertion region. Virology 202:771–781
- Haywood A. M. 1994; Virus receptors: binding, adhesion strengthening and changes in viral structure. Journal of Virology 68:1–5
- Jacobs S. C. 1993; A novel recombinant adenovirus vector expressing a flavivirus non-structural protein protects against lethal flavivirus challenge. Clinical Science 85:117–122
- Jacobs S. C., Stephenson J. R., Wilkinson G. W. G. 1994; Protection elicited by a replication-defective adenovirus vector expressing the tick-borne encephalitis virus non-structural glycoprotein NS1. Journal of General Virology 75:2399–2402
- Kasseisler A., Falck-Pedersen E., Elfenbeim D. H., Alvira M., Buttrick P. M., Leinwand L. A. 1994; The impact of developmental stage, route of administration and the immune system on adenovirus-mediated gene transfer. Gene Therapy 1:395–402
- Letellier C., Burny A., Meulemans G. 1991; Construction of a pigeonpox virus recombinant: expression of the Newcastle disease virus (NDV) fusion glycoprotein and protection of chickens against NDV challenge. Archives of Virology 118:43–56
- Li P., Bellett A. J. D., Parish C. R. 1984; DNA-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses. Journal of General Virology 65:1817–1825
- Louis N., Fender P., Barge A., Kitts P., Chroboczek J. 1994; Cell binding domain of adenovirus type 2 fiber. Journal of Virology 68:4104–4106
- Mathias P., Wicham T., Moore M., Nemerow G. 1994; Multiple adenovirus serotypes use alpha v integrins for infection. Journal of Virology 68:6811–6814
- MacPherson I., Stokes M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
- Morgan R. W., Gelb J., Schreurs C. S., Lutticken D., Rosenberger J. K., Sondermeijer P. J. A. 1992; Protection of chickens from Newcastle and Marek’s diseases with a recombinant herpesvirus of turkeys vaccine expressing the Newcastle disease virus fusion protein. Avian Diseases 36:858–870
- Natuk R. J., Lubeck M. D., Chanda P. K., Chengalvala M., Wade M. S., Murthy S. C., Whilhelm J., Vernon S. K., Dheer S. K., Mizutani S. 1993; Immunogenicity of recombinant human adenovirus-human immunodeficiency virus vaccines in chimpanzees. AIDS Research and Human Retroviruses 9:395–404
- Oualikene W., Gonin P., Eloit M. 1994; Short and long term dissemination of deletion mutants of adenovirus in permissive (cotton rat) and non-permissive (mouse) species. Journal of General Virology 75:2765–2768
- Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. 1989; Use of human adenosine-based vectors for antigen expression in animals. Journal of General Virology 70:429–134
- Prevec L., Christie B. S., Laurie K. E., Bailey M. M., Graham F.L., Rosenthal K. L. 1991; Immune response to HIV-1 gag antigens induced by recombinant adenovirus in mice and rhesus macaque monkeys. Journal of Acquired Immunodeficiency Syndromes 4:568–576
- Ragot T., Finerty S., Watkins P. E., Perricaudet M., Morgan A. J. 1993; Replication-defective recombinant adenovirus expressing the Epstein-Barr virus (EBV) envelope glycoprotein gp340/220 induces protective immunity against EBV-induced lymphomas in cottontop tamarin. Journal of General Virology 74:501–507
- Seth P. 1994; Adenovirus-dependent release of choline from plasma membrane vesicles at an acidic pH is mediated by the penton base protein. Journal of Virology 68:1204–1206
- Seth P., Rosenfeld M., Higginbotham J., Crystal R. G. 1994; Mechanism of enhancement of DNA expression consequent to cointernalization of a replication-deficient adenovirus and unmodified plasmid DNA. Journal of Virology 68:933–940
- Sondermeijer P. J. A., Claessens J. A. J., Jenniskens P. E., Mocket A. P. A., Thijssen R. A. J., Willemse M. J., Morgan R. W. 1993; Avian herpesvirus as a live viral vector for the expression of heterologous antigens. Vaccine 11:349–357
- Taylor J., Edbauer C., Rey-Senelongue A., Bouquet J. F., Norton E., Goebel S., Desmettre P., Paoletti E. 1990; Newcastle disease virus fusion protein expressed in a fowlpox virus recombinant confers protection in chickens. Journal of Virology 64:1441–1450
- Taylor J., Weinberg R., Tartaglia J., Richardson C., Alkhatib F., Briedis D., Appel M., Norton E., Paoletti E. 1992; Nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins. Virology 187:321–328
- Vanner P., Hutet E., Bourgueil E., Carlolet R. 1991; Level of virulent virus excreted by infected pigs previously vaccinated with different glycoprotein deleted Aujeszky’s disease vaccines. Veterinary Microbiology 29:211–223
- Webster R. G., Kawaoka Y., Taylor J., Weinberg R., Paoletti E. 1991; Efficacy of nucleoprotein and hemagglutinin antigens expressed in fowlpox virus as a vaccine for influenza in chickens. Vaccine 9:303–308
- Yanagida N., Ogawa R., Lee Y., Nazerian K. 1992; Recombinant fowlpox viruses expressing the glycoprotein B homolog and the pp38 gene of Marek’s disease virus. Journal of Virology 66:1402–1408
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