An Extensive Class of Small RNAs in Caenorhabditis elegans
Abstract
The lin-4 and let-7 antisense RNAs are temporal regulators that control the timing of developmental events inCaenorhabditis elegans by inhibiting translation of target mRNAs. let-7 RNA is conserved among bilaterian animals, suggesting that this class of small RNAs [microRNAs (miRNAs)] is evolutionarily ancient. Using bioinformatics and cDNA cloning, we found 15 new miRNA genes in C. elegans. Several of these genes express small transcripts that vary in abundance during C. elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse.
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REFERENCES AND NOTES
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We thank A. Lee for computer programming, M. McCarthy for worm staging, D. Jewell for helping with BLAST searches, the Sanger Center for C. briggsae genomic sequence, and W. J. Kent's Intronerator Web site for presorted worm sequences. We are also grateful to A. Grishok and C. Mello for the dcr-1 strain and to T. Tuschl and D. Bartel for sharing data prior to publication. This work was supported by NIH grant R01 GM-34028 to V.A.
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Published In
Science
Volume 294 | Issue 5543
26 October 2001
26 October 2001
Submission history
Received: 13 August 2001
Accepted: 18 September 2001
Published in print: 26 October 2001
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- Rosalind C. Lee,
- Victor Ambros
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