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Differentiated Rat Glial Cell Strain in Tissue Culture

Philippe Benda, James Lightbody, Gordon Sato, Lawrence Levine, and William SweetAuthors Info & Affiliations
Science
26 Jul 1968
Vol 161, Issue 3839
pp. 370-371
DOI: 10.1126/science.161.3839.370
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Abstract

Rat glial tumors, induced by injections of N-nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.

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Science
Volume 161 | Issue 3839
26 July 1968

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© 1968.

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Published in print: 26 July 1968

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Authors

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Philippe Benda
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts
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James Lightbody
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts
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Gordon Sato
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts
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Lawrence Levine
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts
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William Sweet
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts
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  • Philippe Benda et al.
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Differentiated Rat Glial Cell Strain in Tissue Culture.Science161,370-371(1968).DOI:10.1126/science.161.3839.370

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