Evolutionary Transition toward Defective RNAs That Are Infectious by Complementation
ABSTRACT
Passage of foot-and-mouth disease virus (FMDV) in cell culture resulted in the generation of defective RNAs that were infectious by complementation. Deletions (of nucleotides 417, 999, and 1017) mapped in the L proteinase and capsid protein-coding regions. Cell killing followed two-hit kinetics, defective genomes were encapsidated into separate viral particles, and individual viral plaques contained defective genomes with no detectable standard FMDV RNA. Infection in the absence of standard FMDV RNA was achieved by cotransfection of susceptible cells with transcripts produced in vitro from plasmids encoding the defective genomes. These results document the first step of an evolutionary transition toward genome segmentation of an unsegmented RNA virus and provide an experimental system to compare rates of RNA progeny production and resistance to enhanced mutagenesis of a segmented genome versus its unsegmented counterpart.
Mutation, recombination, and ensuing phenotypic modifications in response to selective pressures can be readily observed and quantitated with RNA viruses both in cell culture and in vivo within modest time periods. This has rendered viruses suitable experimental systems for studies of basic Darwinian processes of genetic modification, competition, and selection or random drift as agents of genome diversification and biological adaptation (reviewed in references 1, 8, and 12). However, some major evolutionary transitions such as RNA genome segmentation have never been observed in the laboratory, yet they have probably occurred, given the hundreds of animal, plant, bacterial, and fungal viruses with segmented RNA genomes that have been described, including picornavirus-like plant RNA viruses (43). We have carried out extensive studies on the population dynamics of the important animal picornavirus foot-and-mouth disease virus (FMDV), including that of phenotypic evolution upon long-term serial cytopathic infections of BHK-21 cells (2). Unexpectedly, after more than 200 serial infections of viral population C-S8c1 of FMDV (a clone of serotype C [reviewed in reference 33]), defective genomes became dominant in the population. Defective interfering (DI) particles are frequently produced upon passage of RNA viruses at a high multiplicity of infection (MOI). DI particles are deletion mutants that require the presence of helper virus for replication and can interfere with the replication of the standard infectious virus (14-16, 29, 32, 35). The results described here show that defective genomes which individually could not cause cytopathology could nevertheless complement each other to produce progeny and kill cells in the absence of standard virus. The results provide, to our knowledge, the first description of defective RNA genomes occurring during viral replication that can be stably maintained by complementation upon passage at a high MOI in the absence of standard infectious virus. Therefore, the results provide experimental evidence of the initial step of an evolutionary transition towards genome segmentation of an unsegmented RNA virus.
MATERIALS AND METHODS
Cells, viruses, and infections.
The origin of baby hamster kidney 21 (BHK-21) cells and procedures for cell growth, infection of cell monolayers with foot-and-mouth disease virus (FMDV) in liquid medium, and for plaque assays in semisolid agar medium have been previously described (3, 9, 38). FMDV C-S8c1 is a plaque-purified virus of the European serotype C, natural isolate C1 Santa-Pau Spain 70 (38). FMDV C-S8p260 is a viral population obtained after 260 serial cytolytic passages of C-S8c1 at high MOIs in BHK-21 cells (2 × 106 BHK-21 cells infected with the virus contained in 200 μl of the supernatant from the previous infection). FMDV C-S8p260p3d is a viral population obtained after three serial cytolytic passages of C-S8p260 at low MOIs in BHK-21 cells (2 × 106 BHK-21 cells infected with 200 μl of a 10−3 dilution of the supernatant from the previous infection). FMDV MARLS is a monoclonal antibody escape mutant obtained from FMDV C-S8c1 passaged 213 times in BHK-21 cells (6). The fitness of FMDV C-S8p260p3d and MARLS relative to C-S8c1 is 70 and 130, respectively (28; J. García-Arriaza, unpublished results).
Cell killing assay.
Cell killing was quantified essentially as previously described (36) and consisted in determining the minimum number of viral particles required to kill 104 BHK-21 cells after variable times of infection. The cells were infected with serial dilutions of the viruses to be tested (the number of viral particles added was determined by real-time PCR with a LightCycler [Roche] instrument), and at different times postinfection cell monolayers were fixed with 2% formaldehyde and stained with 2% crystal violet in 2% formaldehyde.
FMDV purification.
FMDV particles were purified as previously described (7). Briefly, virus was concentrated through a sucrose cushion and then resuspended in TNE (0.1 M Tris [pH 7.5], 0.05 M EDTA, 0.5 M NaCl) and sedimented through a sucrose gradient (7.5 to 30% in TNE). Fractions of 700 μl were collected for analysis.
Quantification of viral particles by electron microscopy.
Purified virus was mixed with an equal volume of a solution of latex beads (91-nm diameter; 1.37 × 1012 latex beads/ml) (Balzers Union). The samples were adsorbed for 2 to 3 min to copper grids coated with collodion-carbon and ionized. The grids were then fixed with 2% glutaraldehyde, negatively stained with 2% uranyl acetate, and air dried. The grid was observed in a transmission electron microscope (JEM1010; Jeol, Tokyo, Japan), and the numbers of viral particles and latex beads were counted in multiple, independent images. The number and size of particles were determined, and standard deviations were calculated, as detailed in Results (see also Fig. 3C).
RNA quantification, cDNA synthesis, PCR amplification, and nucleotide sequencing.
Viral RNA was extracted from supernatants of infected cells, purified virus, or individual viral plaques by treatment with Trizol (Invitrogen) as previously described (37). RNA quantification was performed by real-time PCR by using the LightCycler instrument with the LightCycler RNA Master SYBR Green I kit or LightCycler RNA Amplification SYBR Green I kit (both from Roche) for small (<500 bp) or large (>500 bp) DNA fragments, respectively. Reverse transcription (RT) was carried out by using avian myeloblastosis virus reverse transcriptase (Promega), and PCR amplification was performed by using either Ampli-Taq polymerase (Perkin-Elmer) or an Expand High Fidelity DNA polymerase system (Roche), as specified by the manufacturers. Amplification products were analyzed by 1% agarose gel electrophoresis and ethidium bromide staining. Nucleotide sequencing was carried out as previously described (10, 11). The positions of the primers in the FMDV genome will be given upon request (the GenBank accession number for the C-S8c1 genomic sequence is AJ133357 [42]).
Construction of an infectious clone of FMDV C-S8c1 and of derivatives containing defective genomes.
An infectious clone of FMDV C-S8c1 was constructed by recombining subclones that represented the whole genome except the poly(C) tract (41) into a pGEM-1 plasmid under the control of the SP6 promoter. A short poly(C) tract was obtained from the genomic RNA by amplification of a 561-nucleotides DNA fragment with Vent DNA polymerase by using 76°C as the hybridization and elongation temperature. A unique NdeI site was introduced at the 3′ end of the viral genome after a six adenylates tract. The clone was optimized by eliminating three C residues located between the SP6 promoter and the FMDV sequence, increasing the poly(C) tract length to 35 Cs and increasing the 3′-terminal poly(A) length from 6 to 25 residues. This infectious clone is named pMT28 and the specific infectivity of its transcript is 105 PFU/μg of RNA. Plasmids pMTΔ417 and pMTΔ999 were constructed by substituting the region spanning nucleotides 436 to 4201 of pMT28 with the corresponding region from the defective genomes Δ417 and Δ999, respectively. The restriction sites used for the construction were HpaI (position 436) and BglII (position 4201) present in pMT28 as unique recognition sites. Procedures for the purification of plasmids, transformation of Escherichia coli DH5α competent cells, and isolation of bacterial colonies have been previously described (3, 37).
In vitro RNA transcription and cell transfection.
Plasmids including FMDV cDNA with deletions (pMTΔ417 and pMTΔ999) were linearized at the NdeI site, and RNA transcription by SP6 polymerase was performed as previously described (3). BHK-21 cells were transfected with RNA transcripts by electroporation, as previously described (3, 17). Briefly, 0.5 ml of BHK-21 cells at a concentration of 106 to 107 cells/ml were mixed with 12 μg of RNA in a 4-mm cuvette. The cells were then pulsed twice at 280 V, 400 Ω of resistance, and a capacitance of 250 μF, with a Bio-Rad Gene Pulser. After electroporation the cells were allowed to attach to tissue culture plates in the presence of Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum. The dead cells and medium were removed after 4 h, and fresh medium with 2% fetal calf serum was added to the cultures, followed by incubation at 37°C.
RESULTS
Defective genomes in a multiply passaged FMDV population.
Clone FMDV C-S8c1 was serially passaged at high MOIs in BHK-21 cells, and at passage 143 (population termed C-S8p143) a defective genome dependent on helper, standard FMDV, was detected in the viral population (6). To investigate the evolution of this defective genome, the virus was further passaged at high MOIs, and at passage 260 (population C-S8p260) viral RNA was extracted from the culture medium and analyzed. C-S8p260 RNA was amplified by RT-PCR by using different pairs of primers which covered the entire C-S8c1 genome (Fig. 1A and B). Amplifications that spanned the L protease- and capsid-coding regions detected three RNA molecules smaller than the standard C-S8c1 genome, suggesting the presence of genomes with deletions. Nucleotide sequencing identified three classes of deletions. One class of RNA contained a deletion of 417 nucleotides within the L protease-coding region, and two other classes of RNA contained deletions of 999 or 1,017 nucleotides in the capsid-coding region. These RNAs are termed Δ417, Δ999, and Δ1017, respectively. The Δ417 deletion involves positions 1153 to 1571 (FMDV genome numbering follows the method of reference 10), and it was already present in C-S8p143 (6). The Δ999 deletion spans positions 2793 to 3793, and Δ1017 spans positions 1932 to 2950 (Fig. 1A). Deletions Δ999 and Δ1017 overlap by 156 nucleotides. The deletions did not alter the open reading frame of the aphthovirus genome. The presence of these three classes of deletions was confirmed by using different independent preparations of RNA from C-S8p260 as template. No RNA molecules containing more than one deletion were detected.
To determine the relative abundance of RNA of standard size and RNA with deletions in C-S8p260, the different classes of RNA were quantified by real-time PCR (LightCycler) by using different pairs of primers specific for each genome class (Table 1). In C-S8p260 there was the same amount of Δ417, Δ999, and Δ1017 RNA estimated in 105 RNA molecules per infected cell, and no standard RNA was detected. Amplifications of serial dilutions of a standard RNA indicated that in C-S8p260 there was at least 10,000 times less standard FMDV RNA than genomes with deletions. To rescue standard genomes that could be present in the population, C-S8p260 was serially passaged three times at low MOIs to yield preparation C-S8p260p3d which contained standard size genomes and no RNAs with deletions (Fig. 1A and B). This result suggests that the genomes with deletions are defective and that standard genomes were present as a minority in C-S8p260.
Viral production by FMDV C-S8p260 and C-S8p260p3d.
Many infections of approximately 104 viral particles per cell indicated that viral production in infections with C-S8p260 was consistently lower (average of 0.97 ± 0.78 PFU/cell) than with C-S8p260p3d (average of 187 ± 96 PFU/cell) (each value is the average of at least 15 determinations). Despite the lower yield, C-S8p260 produced complete cytopathology at approximately the same time as C-S8p260p3d (6 to 9 h postinfection). The plaque size for C-S8p260 and C-S8p260p3d on BHK-21 cell monolayers was 0.94 ± 0.53 and 2.68 ± 0.86 mm, respectively (average of measurements of 100 plaques) (Fig. 1C). These data indicate differences in the capacity to produce viral progeny of C-S8p260 and C-S8p260p3d, despite producing a similar cytopathology.
Particles with defective RNAs form plaques on BHK-21 cell monolayers.
Viral populations C-S8p260 and C-S8p260p3d were plated on BHK-21 monolayers, and single plaques were isolated. The nature of the RNAs contained in the individual plaques was analyzed by RT-PCR (Table 2). A total of 61 of 62 plaques obtained from C-S8p260 contained Δ417 and Δ999 or Δ1017 RNA and no detectable standard RNA. Some of these plaques were obtained from a second or a third replating. This result strongly suggests that the cytopathology associated with plaque formation was produced by RNAs containing deletions, in the absence of standard RNA. As expected, C-S8p260p3d yielded only plaques containing virus with standard size genomes. One out of 42 plaques isolated from a first plaque assay of C-S8p260 contained a standard genome as its only genome. This result again indicates the existence of standard length genomes as a minority in population C-S8p260, which upon three serial passages at low MOIs originated population C-S8p260p3d, which is devoid of detectable defective genomes.
Cell killing by C-S8p260 and C-S8p260p3d.
An FMDV cell killing assay for BHK-21 cells (reference 36 and Materials and Methods) revealed a very significant difference in the behavior of FMDV populations C-S8p260 and C-S8p260p3d (Fig. 2). The time needed to kill 104 cells depended logarithmically on the number of viral particles added for C-S8p260p3d, MARLS, and C-S8c1 (the latter two FMDV variants, described in Materials and Methods, were included to control the effect of relative fitness on cell killing). However, for C-S8p260, such dependence was a power-law function. A theoretical model (unpublished data) that relates the time needed for cell killing (T), the initial number of viral particles (no), and the fraction of infectious particles produced after a replication cycle (in a single cell) which can effectively infect new cells, indicates a logarithmic function of T versus no when a single particle is sufficient to kill a cell and a power-law function when two (or more) particles are needed to kill a cell, in agreement with the experimental results. Therefore, in C-S8p260 two particles with defective RNAs are needed to initiate infection of a single cell, suggesting that infection is produced by complementation of the two defective RNAs. The need for complementation provides an interpretation of the fact that the yield of C-S8p260 (measured as PFU/cell) was consistently about 200-fold lower than the yield of C-S8p260p3d, despite a similar time of appearance of cytopathology (see above). Therefore, there is an ambiguity in the definition of PFU in such a complementation system.
Complementation of RNA transcripts containing deletions Δ417 and Δ999 in the absence of standard RNA.
To prove that Δ417 and Δ999 RNAs can infect by complementation, BHK-21 cells were transfected with either Δ417 RNA, Δ999 RNA, or the two RNAs together. The RNAs were transcribed from plasmids pMTΔ417 and pMTΔ999. The presence or absence of cytopathology of transfected cells was monitored, and samples of cell culture supernatants at different times posttransfection were analyzed for the presence of defective RNAs or standard RNAs and for infectivity (Table 3). Each Δ417 or Δ999 RNA transcript alone failed to produce cytopathology of BHK-21 cells at 5 days posttransfection. Nor was cytopathology observed upon blind infection of BHK-21 cells with supernatants of transfected cells (see Discussion). In contrast, at 5 days after cotransfection with a mixture of Δ417 and Δ999 RNAs, cytopathology of the electroporated cells was observed and only RNAs Δ417 and Δ999 were detected. At 72 h after cotransfection, although no cytopathology was yet observed, the supernatant of BHK-21 cells cotransfected with Δ417 and Δ999 RNAs produced cytopathology at 5 days postinfection of fresh BHK-21 cells. Again, only Δ417 and Δ999 RNAs were detected as progeny RNAs. Thus, Δ417 and Δ999 RNAs are defective RNAs, and they produce infection by complementation in the absence of standard RNA.
Defective RNAs are encapsidated in particles and each particle contains one defective genome.
To determine whether defective RNAs are packaged in viral particles, C-S8p260 and C-S8p260p3d were sedimented through a sucrose gradient, and fractions were analyzed for infectivity and the presence of defective or standard genomes by electron microscopy. Defective RNAs present in C-S8p260 and standard RNA present in C-S8p260p3d comigrated in the sucrose gradient, with maximum infectivity and RNA levels in fractions 13 to 15 (Fig. 3A and B). Electron microscopy examination showed that both populations contained particles of 30 ± 0.1 nm in diameter (Fig. 3C). To determine whether one particle contained one or more defective RNA molecules, the amount of RNA in C-S8p260 (determined by real-time PCR), was compared with the number of particles (calculated by quantitative electron microscopy) (Fig. 3C). The values obtained were 3.6 ± 1.6 × 108 RNA molecules/μl and 5.5 ± 0.8 × 108 viral particles/μl for C-S8p260 and 9.5 ± 0.8 × 108 RNA molecules/μl and 1.5 ± 0.6 × 109 viral particles/μl for C-S8p260p3d (average of 3 to 10 determinations). Therefore, in C-S8p260 each viral particle contains one defective RNA molecule, and in C-S8p260p3d each viral particle contains one standard RNA genome. We conclude that FMDV evolved in cell culture to produce a population dominated by virus particles containing defective RNAs that were infectious by complementation.
DISCUSSION
Defective viral genomes requiring a helper virus for replication are frequently generated upon serial high MOI passage of RNA viruses (14-16, 29, 32, 35). Deletions in most picornavirus DI genomes are located in the 5′ portion of the viral RNA in the capsid protein-coding region (20, 26), and they ranged between 4 and 16% of the genomic residues (21). Packaging constraints dictate the approximate size limits of genome deletions that can be accommodated by viral encapsidation mechanisms (14, 35). In the case of FMDV, defective Δ417 RNA-lacking 417 nucleotides of the L region-was shown to be packaged and to contribute capsid proteins to the particles of the helper virus (6). Engineered FMDV RNA lacking the complete open reading frame of protein Lb produced infectious virus upon transfection of BHK-21 cells (30). O1K FMDV RNA transcripts lacking Lb- and capsid-coding regions were replication competent but their trans encapsidation upon superinfection with homologous virus was inefficient (22). The difference between this O1K-based replicon and the defective RNAs described here in their capacities to produce cytopathology may be influenced by nucleotide sequence context, the nature of the deletions, or a longer time required for cytopathology in transfections with C-S8c1 cDNA transcripts than with O1K cDNA transcripts (Table 3). The observed complementation between FMDV-defective genomes lacking the L protease and some capsid proteins is in agreement with the trans-acting properties of these proteins (22, 23, 30, 39).
Engineered RNAs encoding nonstructural or structural proteins of Sindbis virus could function to produce infectious particles with copackaged bipartite RNA (13). DI RNAs of mouse hepatitis virus could complement engineered RNAs expressing the missing proteins (18). In the DNA virus simian virus 40, a defective DNA encoding mostly early functions could complement another defective DNA encoding late functions to produce infectivity (27). Both DNAs contained iterated replication origins that could contribute to their stability and infectivity.
In the present study we have documented that FMDV can evolve to produce defective genomes that can replicate and kill cells in the absence of wild-type virus. This has been shown by independent procedures such as the presence of defective genomes in individual viral plaques, infection by transcripts of the defective genomes, and two-hit kinetics for cell killing.
What could trigger the evolutionary transition from a high fitness, replication-competent standard FMDV genome toward two RNA forms with deletions that become dominant and are infectious by complementation? Theoretical studies have led to two main proposals for the driving force for genome segmentation. One proposal is that genome segmentation evolved in response to high mutation rates to provide multicomponent reproduction as a form of sex to attenuate the effect of deleterious mutations (5, 31). In this respect, it has been recently reported that genomes of nucleopolyhedrovirus with deletions can work with full-length partners to mutual benefit, possibly through complementation (19). Another proposal is that segmentation could result from selection of shorter RNA molecules that replicate faster than the corresponding parental genome, favored at high MOIs to ensure efficient complementation (25, 40). Deleted forms of viral RNAs have indeed been shown to have a selective replicative advantage over parental full-length genomes in vitro (24, 34). A difference of replication capacity or of tolerance to mutations is now amenable to direct experimental testing with the segmented-unsegmented FMDV genome system, and such experiments are currently in progress.
Nucleotide sequence analyses (unpublished data) suggest that a recombination event within the VP4-coding region could be involved in the transition from the segmented to the unsegmented FMDV genome, a transition which is strongly selected at low MOIs.
It is not possible to know the relevance of these observations in cell culture for the natural evolution of RNA genomes or whether in nature segmented RNA genomes evolved from or preceded unsegmented forms. What our results show, however, is that RNA genomes have an evolutionary potential to evolve towards genome segmentation within infected cells, provided a strong selective pressure to favor complementation (high MOI) is present. A number of plant RNA viruses have segmented genomes encapsidated into separate particles (multipartite genomes) (43). The FMDV provides an experimental system with which to compare possible advantages of segmentation in ways that have not been possible until now because segmented and unsegmented cognate forms can be compared. It will be also intriguing to examine further the evolution of the system dominated by defective RNAs at high MOI passage and to analyze whether additional divergence and functional specialization of the two segments occur. These studies may be relevant for probing the advantage of sex in genetic systems.
FIG. 1.
FIG. 2.
FIG.3.
TABLE 1.
| Amplification no.a | Position of primersb | Size of expected product (bp) | Quantified RNAsc | No. of RNA molecules/μld | ||||
|---|---|---|---|---|---|---|---|---|
| Sense | Antisense | C-S8p260 | C-S8p260p3d | |||||
| 28 | 5344-5363 | 5699-5678 | 356 | total RNA | 2.1 ± 0.6 × 108 | 3.2 ± 0.8 × 108 | ||
| 29 | 1539-1560 | 2926-2903 | 1388 | st | <1.7 ± 0.4 × 104e | 1.7 ± 0.4 × 108 | ||
| 30 | 2744-2770 | 2926-2903 | 183 | st + Δ417 | 1.3 ± 0.1 × 108 | 1.7 ± 0.4 × 108 | ||
| 31 | 1305-1330 | 2140-2122 | 836 | st + Δ999 | 8.5 ± 3.4 × 107 | 2.2 ± 0.9 × 108 | ||
| 32 | 1539-1560 | 2983-2961 | 1445 + 429 | st + Δ1017 | 8.2 ± 5.8 × 107 | 5.8 ± 4.8 × 108 | ||
| 33 | 1692-1710 | 2140-2122 | 449 | st + Δ417 + Δ999 | 2.2 ± 0.6 × 108 | 2.6 ± 0.6 × 108 | ||
| 34 | 3175-3194 | 3518-3496 | 344 | st + Δ417 + Δ1017 | 1.6 ± 0.2 × 108 | 2.6 ± 0.8 × 108 | ||
| 35 | 1305-1330 | 1619-1596 | 315 | st + Δ999 + Δ1017 | 9.8 ± 2.4 × 107 | 1.9 ± 0.6 × 108 | ||
a
Amplifications were carried out with primers that could amplify specifically each class of RNA. Numbers are correlative to those of the amplifications shown in Fig. 1A.
b
Position refers to the FMDV C-S8c1 genome, with the numbering used in reference 10.
c
Class of RNA quantified by the specific primers. In C-S8p260p3d (free of defective genomes), all the amplifications quantified only standard (st) genomes.
d
Number of RNA molecules per microliter of supernatant of infected cells. 108 RNA molecules/μl corresponds to about 105 molecules per infected cell. Each value is the average of at least two independent determinations and represents the mean of triplicate amplification assays; standard deviations are indicated.
e
No standard RNA was detected in C-S8p260 by real-time PCR (Light Cycler). To ensure that standard (st) RNA could be amplified in the presence of an excess of RNAs with deletions amplifications with primers located within the sequences deleted in ΔRNAs (amplification number 29) were carried out with a fixed amount of C-S8p260 RNA (corresponding to 1 μl [≈3.4 ng RNA] of an undiluted RNA preparation extracted from C-S8p260) and 10-fold serial dilutions of C-S8p260p3d (corresponding to RNA extract from C-S8p260p3d). Standard RNA was detected even when present at a 10−4 dilution relative to ΔRNAs; the intensity of the RT-PCR band was about 50% of the intensity observed in the absence of RNAs with deletions. Since the last dilution of C-S8p260p3d in which there was positive amplification was 10−4, in C- S8p260 the maximum amount of st RNA was 1.7×104 molecules per μl of supernatant of infected cells.
TABLE 2.
| Virus and assaya | No. of plaques analyzed | Genomes present in the analyzed plaquesb | ||||
|---|---|---|---|---|---|---|
| st | Δ417 | Δ999 or Δ1017 | ||||
| C-S8p260 | ||||||
| First plaque assay | 41 | − | + | + | ||
| 1 | + | − | − | |||
| Second plaque assay | 17 | − | + | + | ||
| Third plaque assay | 3 | − | + | + | ||
| C-S8p260p3d plaque assay | 5 | + | − | − | ||
a
The origin of C-S8p260 and C-S8p260p3d and the procedure for the plaque assays are described in Materials and Methods. Three serial plaque assays were done with various plaques from C-S8p260.
TABLE 3.
| Parameter | RNA at times post-transfectiona | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pMTΔ417 + pMTΔ999 | pMTΔ417 | pMTΔ999 | |||||||||||||||||
| 0 h | 2 h | 4 h | 16 h | 24 h | 36 h | 72 h | 5 days | 5 days | 5 days | ||||||||||
| Cytopathologyb | − | − | − | − | − | − | − | + | − | − | |||||||||
| RT-PCR of RNA from trans- fection supernatantsc | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417, Δ999 | Δ417 | Δ999 | |||||||||
| Infectiond | − | − | − | − | − | − | + | + | − | − | |||||||||
| RT-PCR of RNA from infection supernatantse | ND | ND | ND | ND | ND | ND | Δ417, Δ999 | Δ417, Δ999, stf | ND | ND | |||||||||
a
Construction of plasmids containing genomes with deletions Δ417 and Δ999 (pMTΔ417 and pMTΔ999, respectively), in vitro transcription, and BHK-21 cell transfection are described in Materials and Methods. Boldface type indicates the presence of complementation of defective RNAs.
b
Cytopathology of 106 cells at the indicated times posttransfection; +, >90% cell killing; −, no detectable cytopathology. In transfections with about 500 ng of an RNA transcript of plasmid pMT28 (standard C-S8c1 RNA, described in Materials and Methods) complete cytpathology is observed between 48 and 72 h posttransfection; the equivalent time for O1K transcripts was about 12 h.
c
The amplifications used were numbers 10, 17, and 21 (Fig. 1A) and 29 (Table 1). At 5 days posttransfection, Δ417 and Δ999 RNA levels in the cotransfection supernatants were about 1.0×104 molecules of RNA per cell, versus 1.4× 104 at time zero after coelectroporation (determined by real-time PCR) (see Materials and Methods). In transfections with Δ999 alone, the amount of RNA was about 7 × 101 molecules per cell at 5 days posttransfection, versus 5×103 molecules per cell at time zero after electroporation.
d
Infection of 104 BHK-21 cells with the supernatant obtained at different times posttransfection is indicated. +, >90% cell killing; −, no detectable cytopathology.
e
f
Sequence analysis revealed that the standard (st) RNA probably arose by recombination of Δ417 and Δ999 RNAs (data not shown; see Discussion).
Acknowledgments
We are indebted to J. J. Holland for valuable suggestions, to M. T. Rejas for the electron microscopy analysis, and to M. Dávila for able technical assistance.
This work was supported by grant BMC2001.1823 C02-01 from MCyT and grant QLRT-2001-00825 from the EU and Fundación Ramón Areces. J.G.-A. was supported by a predoctoral fellowship from MSyC.
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© 2004 American Society for Microbiology.
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Received: 4 March 2004
Accepted: 14 June 2004
Published online: 1 November 2004
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2004. Evolutionary Transition toward Defective RNAs That Are Infectious by Complementation. J Virol 78:.
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