Dissemination of the Phage-Associated Novel Superantigen Gene speL in Recent Invasive and Noninvasive Streptococcus pyogenes M3/T3 Isolates in Japan
ABSTRACT
MATERIALS AND METHODS
Bacterial strains.
PFGE.
Lambda library construction.
PCR.
Preparation of phage DNA.
Nucleotide sequencing.
Nucleotide sequence accession number.
RESULTS
PFGE analysis of S. pyogenes M3/T3 strains.
Cloning of 40-kb fragment.
Distribution of speL.
Induction of φNIH1.1.
DISCUSSION
Strain | M type/ T type | spe profile(s) | Mutation(s) in emm3 from base 81 to base 550a | Isolation year | Specimen | Remarks/diagnosisb |
---|---|---|---|---|---|---|
ATCC 10389 | M3/T3 | B | 1937 | Richards, D58, puerperal septicemia (13, 20, 28) | ||
D58X/11/1 | M3/T3 | B | 328 G to T, 329 A to C | 20 | ||
SS-265 | M3/T3 | B | Before 1956 | |||
Lewis | Untypable/T3 | ABC | 7-base insertion between 210 A and 211 T; 415 G to A | Before 1958 | 20 | |
B930/24/3 | M3/T3 | AB | 245 G to A | 1952 | Throat swab | Rheumatic fever (20) |
K21 | M3/T3 | AB | 415 G to A | 1973 | Throat swab | Pharyngitis |
K22 | M3/T3 | AB | 134 G to A, 341 A to G | 1973 | Throat swab | Pharyngitis |
K23 | M3/T3 | AB | 134 G to A, 341 A to G | 1973 | Throat swab | Pharyngitis |
K24 | M3/T3 | AB | 134 G to A | 1973 | Throat swab | Pharyngitis |
K25 | M3/T3 | AB | 134 G to A | 1973 | Throat swab | Pharyngitis |
K31 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Throat swab | Pharyngitis |
K32 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Throat swab | Pharyngitis |
K33 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Throat swab | Pharyngitis |
K34 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Throat swab | Pharyngitis |
K35 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Throat swab | Pharyngitis |
NIH1 | M3/T3 | AB | 215 A to C, 245 G to A | 1992 | Fascia | TSLS (27) |
NIH3 | M3/T3 | AB | 215 A to C, 245 G to A | 1993 | Blood | Puerperal TSLS |
NIH7 | M3/T3 | AB | 215 A to C, 245 G to A | 1993 | Blood | TSLS, LC, HCC |
NIH8 | M3/T3 | AB | 215 A to C, 245 G to A | 1993 | Blood | TSLS |
NIH9 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Blood | TSLS |
NIH12 | M3/T3 | AB | 215 A to C, 245 G to A, 371 C to T | 1994 | Blood | Cellulitis |
NIH14 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Lower limb soft tissue | TSLS |
NIH18 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Lower limb soft tissue | TSLS |
NIH20 | M3/T3 | AB | 215 A to C, 245 G to A | 1995 | Bullous fluid | TSLS |
NIH33 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Upper limb soft tissue | Necrotizing fasciitis, cutaneous lymphoma |
NIH34 | M3/T3 | AB | 215 A to C, 245 G to A | 1994 | Blood | TSLS |
NIH152 | M3/T3 | AB | 215 A to C, 245 G to A | 2001 | Lower limb soft tissue | TSLS, LC |
NIH158 | M3/T3 | AB | 215 A to C, 245 G to A | 2001 | Bullous fluid | TSLS |
Acknowledgments
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