Cellular properties of extensor carpi radialis brevis and trapezius muscles in healthy males and females
Publication: Canadian Journal of Physiology and Pharmacology
6 May 2015
Abstract
In this study, we sought to determine whether differences in cellular properties associated with energy homeostasis could explain the higher incidence of work-related myalgia in trapezius (TRAP) compared with extensor carpi radialis brevis (ECRB). Tissue samples were obtained from the ECRB (n = 19) and TRAP (n = 17) of healthy males and females (age 27.9 ± 2.2 and 28.1 ± 1.5 years, respectively; mean ± SE) and analyzed for properties involved in both ATP supply and utilization. The concentration of ATP and the maximal activities of creatine phosphokinase, phosphorylase, and phosphofructokinase were higher (P < 0.05) in ECRB than TRAP. Succinic dehydrogenase, citrate synthase, and cytochrome c oxidase were not different between muscles. The ECRB also displayed a higher concentration of Na+–K+-ATPase and greater sarcoplasmic reticulum Ca2+ release and uptake. No differences existed between muscles for either monocarboxylate transporters or glucose transporters. It is concluded that the potentials for high-energy phosphate transfer, glycogenolysis, glycolysis, and excitation–contraction coupling are higher in ECRB than TRAP. Histochemical measurements indicated that the muscle differences are, in part, related to differing amounts of type II tissue. Depending on the task demands, the TRAP may experience a greater metabolic and excitation–contraction coupling strain than the ECRB given the differences observed.
Résumé
Cette étude avait pour objectif de déterminer si des propriétés dissemblables de l’homéostasie cellulaire pourraient expliquer l’augmentation de la fréquence de myalgies associées à l’effort dans l’extenseur radial du carpe (ERC) comparativement au trapèze (TPZ). Nous avons étudié les propriétés liées à l’approvisionnement en ATP et à son utilisation dans des échantillons de tissu d’ERC (n = 19) et de TPZ (n = 17) prélevés chez des hommes et des femmes en bonne santé (âgés de 27,9 ± 2,2 et de 28,1 ± 1,5 ans [moyenne ± ÉT], respectivement). La concentration d’ATP et les activités maximales de la créatine phosphokinase, de la phosphorylase et de la phosphofructokinase étaient plus élevées (P < 0,05) dans l’ERC que dans le TPZ, alors que celles de la déshydrogénase succinique, de la citrate synthase et de la cytochrome c oxydase étaient semblables dans les deux types de muscle. En outre, les concentrations de Na+–K+-ATPase ainsi que les taux de libération et de recapture de l’ion Ca2+ étaient plus élevés dans le réticulum sarcoplasmique de l’ERC que dans celui du TPZ, alors que les caractéristiques des transporteurs de monocarboxylates et des transporteurs du glucose étaient semblables dans les deux types de muscle. Nous en arrivons à la conclusion que les potentiels de transfert de phosphates riches en énergie, de glycogénolyse, de glycolyse et de couplage excitation–contraction sont plus élevés dans l’ERC que dans le TPZ. Les mesures histochimiques indiquent que ces différences entre les 2 types de muscle sont, en partie, liées aux disparités entre les quantités de tissu de type II. Étant donné les différences observées, des contraintes plus importantes pourraient être imposées au TPZ qu’à l’ERC sur le plan métabolique et du couplage excitation–contraction selon les exigences des tâches. [Traduit par la Rédaction]
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Published In
Canadian Journal of Physiology and Pharmacology
Volume 93 • Number 11 • November 2015
Pages: 953 - 966
History
Received: 24 December 2014
Accepted: 31 March 2015
Accepted manuscript online: 6 May 2015
Version of record online: 6 May 2015
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© 2015.
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Howard J. Green, Don Ranney, Margaret Burnett, Sobia Iqbal, Natasha Kyle, David Lounsbury, Jing Ouyang, A. Russell Tupling, Ian C. Smith, Riley Stewart, and Heather Tick. 2015. Cellular properties of extensor carpi radialis brevis and trapezius muscles in healthy males and females. Canadian Journal of Physiology and Pharmacology. 93(11): 953-966. https://doi.org/10.1139/cjpp-2014-0549