Ancient DNA from 8400 Year-Old Çatalhöyük Wheat: Implications for the Origin of Neolithic Agriculture
Fig 3
Autoradiograph of the radioactively labeled PCR amplification products of the Çatalhöyük samples separated on DNA sequencing gel.
1) Modern T. durum targeting 243 bp long PCR amplification product of Glu locus. 2) No DNA, negative control of PCR loaded on lane 1. 3) PCR with Çatalhöyük samples extraction blank. 4) PCR with Çatalhöyük62 and 5) Emmer DNA isolates. 6) PCR with Baklatepe sample extraction blank. 7) PCR with a Baklatepe sample. 8) Modern T. durum. 9) No DNA, negative control of PCR loaded on lane 8. 10) PCR with extraction blank. 11) PCR with Çatalhöyük62 and 12) Çatalhöyük61 DNA isolates. 13) PCR with a Baklatepe sample extraction blank. 14) PCR with a Baklatepe sample. B) Blank lanes. Lanes A) DNA sequencing reaction products with ddATP and G) DNA are sequencing reaction products with ddGTP of M13mp18 ssDNA using a T7 primer. The arrows on the left indicate the lengths in bp, the arrows on the right (lanes 11 and 12) indicate the top and bottom alleles in the Çatalhöyük samples.