TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
Corresponding Author
I. Badano
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Correspondence: Ines Badano, PhD., Laboratorio de Biología Molecular Aplicada, Universidad Nacional de Misiones, Av. Mariano Moreno 1375 - Posadas, Misiones 3300, Argentina. Tel/Fax: +54 376 4435118; E-mail: [email protected]Search for more papers by this authorT. G. Schurr
Laboratory of Molecular Anthropology, Department of Anthropology, University of Pennsylvania, Philadelphia, PA, USA
Search for more papers by this authorS. M. Stietz
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorM. C. Dulik
Laboratory of Molecular Anthropology, Department of Anthropology, University of Pennsylvania, Philadelphia, PA, USA
Search for more papers by this authorM. Mampaey
Ministerio de Salud Pública de la Provincia de Misiones, Misiones, Argentina
Search for more papers by this authorI. M. Quintero
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorJ. B. Zinovich
Ministerio de Salud Pública de la Provincia de Misiones, Misiones, Argentina
Search for more papers by this authorR. H. Campos
Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Search for more papers by this authorD. J. Liotta
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorCorresponding Author
I. Badano
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Correspondence: Ines Badano, PhD., Laboratorio de Biología Molecular Aplicada, Universidad Nacional de Misiones, Av. Mariano Moreno 1375 - Posadas, Misiones 3300, Argentina. Tel/Fax: +54 376 4435118; E-mail: [email protected]Search for more papers by this authorT. G. Schurr
Laboratory of Molecular Anthropology, Department of Anthropology, University of Pennsylvania, Philadelphia, PA, USA
Search for more papers by this authorS. M. Stietz
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorM. C. Dulik
Laboratory of Molecular Anthropology, Department of Anthropology, University of Pennsylvania, Philadelphia, PA, USA
Search for more papers by this authorM. Mampaey
Ministerio de Salud Pública de la Provincia de Misiones, Misiones, Argentina
Search for more papers by this authorI. M. Quintero
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorJ. B. Zinovich
Ministerio de Salud Pública de la Provincia de Misiones, Misiones, Argentina
Search for more papers by this authorR. H. Campos
Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Search for more papers by this authorD. J. Liotta
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Search for more papers by this authorSummary
The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the white-admixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.
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