Abstract
The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2'-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro. In a trm7Delta strain, which is viable but grows slowly, translation is impaired, thus indicating that these tRNA modifications could be important for translation efficiency. We discuss the emergence of a family of three 2'-O-RNA methyltransferases in Eukaryota and one in Prokaryota from a common ancestor. We propose that each eukaryotic enzyme is located in a different cell compartment, in which it would methylate a different RNA that can adopt a very similar secondary structure.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Amino Acid Sequence
- Animals
- Anticodon / metabolism*
- Base Sequence
- Catalysis
- Cell Cycle Proteins / chemistry
- Escherichia coli / enzymology
- Eukaryotic Cells
- Humans
- Methyltransferases / chemistry
- Molecular Sequence Data
- Mutagenesis
- Nucleic Acid Conformation
- Protein Biosynthesis
- Protein Structure, Tertiary
- RNA, Fungal / metabolism*
- RNA, Messenger
- RNA, Transfer / metabolism*
- Recombinant Fusion Proteins / chemistry
- Recombinant Fusion Proteins / classification
- Recombinant Fusion Proteins / genetics
- Recombinant Fusion Proteins / metabolism
- Ribose / analogs & derivatives
- Ribose / biosynthesis*
- S-Adenosylmethionine / metabolism
- Saccharomyces cerevisiae / enzymology*
- Saccharomyces cerevisiae / genetics
- Saccharomyces cerevisiae Proteins / chemistry
- Saccharomyces cerevisiae Proteins / classification
- Saccharomyces cerevisiae Proteins / genetics
- Saccharomyces cerevisiae Proteins / metabolism*
- Sequence Homology, Amino Acid
- tRNA Methyltransferases / chemistry
- tRNA Methyltransferases / classification
- tRNA Methyltransferases / genetics
- tRNA Methyltransferases / metabolism*
Substances
- Anticodon
- Cell Cycle Proteins
- RNA, Fungal
- RNA, Messenger
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae Proteins
- D-2-O-methylribose
- Ribose
- S-Adenosylmethionine
- RNA, Transfer
- Methyltransferases
- Trm7 protein, S cerevisiae
- rlmE protein, E coli
- tRNA Methyltransferases