Abstract
Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Acquired Immunodeficiency Syndrome / prevention & control*
- Amino Acid Sequence
- Animals
- Aotidae / genetics*
- Base Sequence
- Capsid Proteins / metabolism
- Chromosome Mapping
- Cyclophilin A / genetics*
- HIV-1 / growth & development*
- Kidney / metabolism*
- Molecular Sequence Data
- Proteins / genetics*
- RNA, Messenger / analysis
- Recombinant Fusion Proteins / genetics*
- Ubiquitin-Protein Ligases
Substances
- Capsid Proteins
- Proteins
- RNA, Messenger
- Recombinant Fusion Proteins
- TRIM5(alpha) protein, rhesus monkey
- Ubiquitin-Protein Ligases
- Cyclophilin A
Associated data
- GENBANK/AY684991
- GENBANK/AY684992
- GENBANK/AY684993
- GENBANK/AY684994
- GENBANK/AY684995
- GENBANK/AY684996
- GENBANK/AY684997