Abstract
The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell invasion protein of Yersinia enterocolitica and the lom-encoded protein of bacteriophage lambda. The similarity of PagC and Ail to Lom leads us to hypothesize that Lom is a virulence protein and that bacteriophage gene transfer and lysogeny could have led to the development of proteins essential to survival within macrophages and eucaryotic cell invasion.
Publication types
- Comparative Study
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Adhesins, Bacterial*
- Amino Acid Sequence
- Bacterial Proteins / genetics*
- Bacterial Proteins / isolation & purification
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Cosmids
- DNA Transposable Elements
- DNA, Bacterial / genetics
- Electrophoresis, Gel, Two-Dimensional
- Genes, Bacterial*
- Information Systems
- Membrane Proteins*
- Molecular Sequence Data
- Restriction Mapping
- Salmonella typhimurium / genetics*
- Salmonella typhimurium / pathogenicity
- Sequence Homology, Nucleic Acid
- Virulence / genetics
- Yersinia enterocolitica / genetics*
- Yersinia enterocolitica / pathogenicity
Substances
- Adhesins, Bacterial
- Bacterial Proteins
- DNA Transposable Elements
- DNA, Bacterial
- Membrane Proteins
- invasin, Yersinia
- pagC protein, Salmonella typhimurium
Associated data
- GENBANK/M55546
- GENBANK/M63255
- GENBANK/M63305
- GENBANK/M63306
- GENBANK/M64332
- GENBANK/S69596
- GENBANK/S69601
- GENBANK/S69604
- GENBANK/S69828
- GENBANK/S69830