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Human sample from Homo sapiens

Identifiers
BioSample: SAMN07710032; Sample name: NCI-PBCF-HTB14.Glass.pson0009; SRA: SRS2545578
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Package
Human; version 1.0
Attributes
isolate ATCC:HTB-14
age 44
biomaterial provider ATCC
sex male
tissue BRAIN
cell line NCI-PBCF-HTB14
cell type EPITHELIAL
culture collection ATCC:HTB-14
disease GLIOBLASTOMA; ASTROCYTOMA
disease stage GRADE IV
karyotype This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found
race White
sample type cell culture
treatment cell line grown on a glass
alias U-87 MG
url Cell culture protocol NCI-PBCF-HTB14:http://pson.cancer.gov/docs/bioresource/brain/NCI-PBCF-HTB14_U-87_MG_SOP-508.pdf
Description

The cells grown for this study were pelleted, and RNA was extracted with an RIN of 9.96. DNA from the pelleted cell sample was genotyped using Promega GenePrint 10 System, which detected the following irregularities: AMEL:Heterozygous Imbalance (Y/X) < 0.1.

BioProject
PRJNA412363
Retrieve all samples from this project

Submission
Leidos Biomedical Research Inc, Pontius Joan; 2017-09-27
Accession:
SAMN07710032
ID:
7710032

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