|
|
| Status |
Public on Mar 07, 2007 |
| Title |
BEAS-2B(B) |
| Sample type |
RNA |
| |
|
| Source name |
Human Bronchial Epithelial Cell
|
| Organism |
Homo sapiens |
| Characteristics |
Histology: immortalized normal
Age:
Sex: M
Race:
|
| Biomaterial provider |
ATCC
|
| Growth protocol |
RPMI 5% BFS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit including DNase1 treatment
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Description |
Immortalized with SV40 Large T antigen
|
| Data processing |
Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250
|
| |
|
| Submission date |
Feb 26, 2007 |
| Last update date |
Nov 13, 2015 |
| Contact name |
Luc Girard |
| E-mail(s) |
Luc.Girard@UTSouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Hamon Center for Therapeutic Oncology Research
|
| Lab |
NB8.114A
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8593 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE4824 |
Analysis of lung cancer cell lines |
|
