|
|
| Status |
Public on Jun 02, 2011 |
| Title |
Bernstein_K562_H3K27ac |
| Sample type |
SRA |
| |
|
| Source name |
K562
|
| Organism |
Homo sapiens |
| Characteristics |
biomaterial_type: immortalized cell line
line: K562
lab: Broad
lab description: Bernstein - Broad Institute
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell organism: human
cell description: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell lineage: mesoderm
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000052
labversion: Illumina_GA2e and Illumina_GA2x
replicate: 1,2
softwareversion: ScriptureVPaperR3
|
| Biomaterial provider |
ATCC
|
| Growth protocol |
K562_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For extraction protocol details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
For data processing details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Submission date |
May 27, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (2) |
| GSE29611 |
Histone Modifications by ChIP-seq from ENCODE/Broad Institute |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| Reanalyzed by |
GSM936088 |
| SRA |
SRX067407 |
| BioSample |
SAMN00622078 |
| Named Annotation |
GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdSig.bigWig |
