|
Status |
Public on Sep 29, 2011 |
Title |
HudsonAlpha_ChipSeq_K562_SP2_(SC-643)_v041610.2 |
Sample type |
SRA |
|
|
Source name |
K562
|
Organism |
Homo sapiens |
Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. Antibody Target: SP2
antibody targetdescription: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-643
controlid: SL1396
labexpid: SL2951,SL3361
replicate: 1,2
softwareversion: MACS
antibody: SP2_(SC-643)
antibody antibodydescription: Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin.
antibody targetdescription: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-643
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.2
protocol description: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid: SL1396
labexpid: SL2951
replicate: 1
replicate description: tier 1
|
Biomaterial provider |
ATCC
|
Treatment protocol |
None
|
Growth protocol |
K562_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Submission date |
Sep 28, 2011 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9052 |
Series (2) |
GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
Relations |
SRA |
SRX100447 |
BioSample |
SAMN00738364 |
Named Annotation |
GSM803402_hg19_wgEncodeHaibTfbsK562Sp2sc643V0416102RawRep1.bigWig |
Named Annotation |
GSM803402_hg19_wgEncodeHaibTfbsK562Sp2sc643V0416102RawRep2.bigWig |