|
|
| Status |
Public on May 22, 2012 |
| Title |
Harvard_ChipSeq_K562_HMGN3_std |
| Sample type |
SRA |
| |
|
| Source name |
K562
|
| Organism |
Homo sapiens |
| Characteristics |
lab: Harvard
lab description: Struhl - Harvard University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: K562
cell organism: human
cell description: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype: cancer
cell lineage: mesoderm
cell sex: F
treatment: None
treatment description: No special treatment or protocol applies
antibody: HMGN3
antibody antibodydescription: Rabbit polyclonal. Raised against an amino acids 60 to 99 of human HMGN3. Vendor recommeds its use for detection of HMGN3 in human samples using immunohistochemistry. Antibody Target: HMGN3
antibody targetdescription: HMGN3 is a high-mobility group protein. These proteins are small, 8 to 11 kDa, ubiquitous proteins believed to play a role in chromatin structure. HMGNs bind to nucleosomes between the histone core and DNA and reduce chromatin compaction. As a result HMGNs, HMGN3 included, are believed to increase access to DNA for DNA replication, repair and transcription. HMGN3 has specifically been implicated in the action of Thyroid horomone receptors.
antibody vendorname: Novus Biologicals
antibody vendorid: NB100-61076
control: std
control description: Standard input signal for most experiments.
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000615
replicate: 1
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
K562_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX150623 |
| BioSample |
SAMN01001083 |
| Named Annotation |
GSM935544_hg19_wgEncodeSydhTfbsK562Hmgn3StdSig.bigWig |
