|
|
| Status |
Public on May 22, 2012 |
| Title |
Harvard_ChipSeq_K562_BDP1_std |
| Sample type |
SRA |
| |
|
| Source name |
K562
|
| Organism |
Homo sapiens |
| Characteristics |
lab: Harvard
lab description: Struhl - Harvard University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: K562
cell organism: human
cell description: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype: cancer
cell lineage: mesoderm
cell sex: F
treatment: None
treatment description: No special treatment or protocol applies
antibody: BDP1
antibody antibodydescription: Rabbit polyclonal - Antiserum 2663 was raised by immunizing rabbits with synthetic peptide CSDRYRIYKAQKLRE (human Bdp1 residues 139?153) coupled to KLH. Antibody Target: BDP1
antibody targetdescription: 'B double-prime 1', subunit of RNA polymerase III transcription initiation factor IIIB
antibody vendorname: Donated by Dr. RJ White, The Beatson Institute for Cancer Research
antibody vendorid: 2663
control: std
control description: Standard input signal for most experiments.
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000615
labversion: fragmented with both a probe sonicator and a Misonix sonicator, and precipitated with protein A sepharose beads
replicate: 1
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
K562_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX150673 |
| BioSample |
SAMN01001133 |
| Named Annotation |
GSM935594_hg19_wgEncodeSydhTfbsK562Bdp1StdSig.bigWig |
