Mycoplasma protocols
In Mycoplasma Protocols, Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenicity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products. Mycoplasma Protocols provides up-to-date and easy-to-follow mycoplasma methods for practical application in medical and veterinary diagnostic and research laboratories. The techniques permit effective work with these normally fastidious microorganisms, allowing investigators to illuminate their roles across a wide range of chronic respiratory, arthritic, and urogenital diseases. For all those working in this important field, Mycoplasma Protocols immediately becomes the methodological resource of choice
Springer Protocols
Laboratory Manual
1 online resource (xiii, 330 pages)
9781592592692, 9780585328553, 9780896035256, 0585328552, 1592592694, 0896035255
45842675
Medical significance of mycoplasmas / Paul Taylor
The veterinary significance of mycoplasmas / Robin Nicholas
Recovery of human mycoplasmas / Paul Taylor
Recovery of mycoplasmas from animals / Robin Nicholas, Samantha Baker
Recovery of mycoplasmas from birds / Janet M. Bradbury
Cultivation of ureaplasmas / Patricia M. Furr
Quality-control testing of mycoplasma medium / David Windsor, Helena Windsor
Biochemical characteristics in mycoplasma identification / José B. Poveda
Enzyme analysis : the rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes / J. Dennis Pollack
Determination of substrate utilization rates by mycoplasmas / Roger J. Miles, Calista Agbanyim
Serological identification of mycoplasmas by growth and metabolic inhibition tests / José B. Poveda, Robin Nicholas
Identification of mycoplasmas by dot immunobinding on membrane filtration (MF Dot) / Francois Poumarat
Identification of mycoplasmas by immunofluorescence / Janet M. Bradbury
Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Hywel J. Ball, David Finlay
Immunohistochemical staining of fixed tissues / Eugenio Scanziani
Extraction of DNA from mycoplasmas / John B. Bashiruddin
Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers / Karl-Erik Johansson, Malin U. Heldtander, Bertil Pettersson
PCR and RFLP methods for the specific detection and identification of Mycoplasma mycoides subsp. mycoides SC / John B. Bashiruddin
Characterization of mycoplasmas by RAPD fingerprinting / Georges A. Rawadi
Classification of isolates by DNA-DNA hybridization / Konrad Sachse, Helmut Hotzel
Insertion sequence analysis / Joachim Frey
Detection of mycoplasmas in cell cultures by cultural methods / Gerald K. Masover, Frances A. Becker
Detection of mycoplasmas in cell cultures by fluorescence methods / Gerald K. Masover, Frances A. Becker
Transformation of mycoplasmas / Chris F. Minion, Paul A. Kapke
Transposon mutagenesis / LeRoy L. Voelker, Kevin Dybvig
Demonstration of extrachromosomal elements / LeRoy L. Voelker, Kevin Dybvig
Expression of foreign genes in Acholeplasma laidlawii / Tanja K Jarhede, Åke Wieslander
Mycoplasma gene expression in Escherichia coli / Chris F. Minion
Polyacrylamide gel-electrophoresis separation of whole-cell proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham
Immunological and biochemical characterization of membrane proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham
Detection and analysis of mycoplasma adhesins / Konrad Sachse
Transmission electron microscopy and immunogold staining of mollicute surface antigens / Gunna Christiansen, Svend Birkelund
Electronic reproduction, [Place of publication not identified], HathiTrust Digital Library, 2010
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