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Cellosaurus Schneider 2 (CVCL_Z232)

[Text version]
Cell line name Schneider 2
Synonyms S2; S-2; SC-2; SL-2; SL2; Line 2; Schneider's Line S2; Schneider's line 2; Schneider Line 2; Schneider L2; Schneider's Drosophila Line2; SCHNEIDER-2; SCHNEIDER S2; Schneider S2; Drosophila Line 2; D. mel. (2); D.Mel-2; D.mel-2; WR69-DM-2; WR-DM-2; Dm2; DL2; S2-ATCC; S2-Invitrogen; S2-RIKEN; S2U
Accession CVCL_Z232
Resource Identification Initiative To cite this cell line use: Schneider 2 (RRID:CVCL_Z232)
Comments Group: Insect cell line.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: ~40 hours (DSMZ=ACC-130).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Children:
CVCL_IZ07 (529SU) CVCL_0Q78 (DeltaWTNdeMYC) CVCL_A8PY (HighMan-S2)
CVCL_Z260 (L-2/M delta 2-3) CVCL_UZ83 (S2 DmECT2-EGFP+Tag-RFP-alpha-tubulin) CVCL_UZ77 (S2 DmMKLP1-EGFP+Tag-RFP-alpha-tubulin)
CVCL_UZ85 (S2 EB1-DeltaC-RacGAP50C-EGFP+Tag-RFP-alpha-tubulin) CVCL_UZ86 (S2 EB1-DeltaPTIV-RacGAP50C-EGFP and TagRFP-alpha-tubulin) CVCL_UZ84 (S2 EB1-RacGAP50C-EGFP+Tag-RFP-alpha-tubulin)
CVCL_UZ80 (S2 EGFP-Rhotekin+Tag-RFP-alpha-tubulin) CVCL_UZ79 (S2 Polo Kinase-EGFP) CVCL_UZ78 (S2 RacGAP50C-EGFP+Tag-RFP-alpha-tubulin)
CVCL_UZ81 (S2 RFP-Rhotekin+DmMKLP1-EGFP) CVCL_UZ82 (S2 RFP-Rhotekin+MRLC-EGFP) CVCL_Z864 (S2-act-GFP-alphaTub84B)
CVCL_IZ08 (S2-B2) CVCL_TZ72 (S2-DGRC) CVCL_Z992 (S2-DRSC)
CVCL_XF59 (S2-GFP-RFP-Atg8a) CVCL_0B20 (S2-Mt-EB1-GFP) CVCL_Z855 (S2-Mt-GFP-Act5C)
CVCL_Z856 (S2-Mt-GFP-alphaTub84B) CVCL_0A56 (S2-SQH-GFP) CVCL_1B57 (S2-Tub-wg)
CVCL_A5UZ (S2:DER2f) CVCL_A5VA (S2:spi4) CVCL_IZ06 (S2c1)
CVCL_Z831 (S2R+) CVCL_Z707 (Sg4)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Schneider_2_cells
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/d/cell-lines-detail-68.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-9.html
https://www.thermofisher.com/order/catalog/product/R69007
http://tools.thermofisher.com/content/sfs/manuals/Drosophila_S2_Cells_UG.pdf
http://www.entu.cas.cz/zurovec/eng/research/cultures.html
https://expressionsystems.com/wp-content/uploads/2017/01/S2-Cells-IFU-Rev-1.pdf
Publications

PubMed=4625067; DOI=10.1242/dev.27.2.353
Schneider I.
Cell lines derived from late embryonic stages of Drosophila melanogaster.
J. Embryol. Exp. Morphol. 27:353-365(1972)

DOI=10.1016/0020-1790(83)90040-9
Kramerov A.A., Metakovsky E.V., Polukarova L.G., Gvozdev V.A.
Glycoprotein patterns in different established cell lines of Drosophila melanogaster responding to 20-hydroxyecdysone.
Insect Biochem. 13:655-663(1983)

DOI=10.2307/3576172
Koval T.M.
Radiosensitivity of cultured insect cells: II. Diptera.
Radiat. Res. 96:127-134(1983)

PubMed=16593348; DOI=10.1073/pnas.80.15.4752
Koval T.M.
Intrinsic resistance to the lethal effects of X-irradiation in insect and arachnid cells.
Proc. Natl. Acad. Sci. U.S.A. 80:4752-4755(1983)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

DOI=10.1016/B978-012229460-0/50004-X
Echalier G.
Drosophila continuous cell lines.
(In) Drosophila cells in culture; Echalier G. (eds.); pp.129-186; Academic Press; New York (1997)

PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2
Grasela J.J., McIntosh A.H.
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)

PubMed=16409117; DOI=10.1290/0412083R.1
McIntosh A.H., Grasela J.J., Popham H.J.R.
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)

PubMed=17417030; DOI=10.1007/978-1-59745-257-1_33
Ceriani M.F.
Basic protocols for Drosophila S2 cell line: maintenance and transfection.
Methods Mol. Biol. 362:415-422(2007)

PubMed=17890361; DOI=10.1534/genetics.107.076356
Williams B.R., Bateman J.R., Novikov N.D., Wu C.-T.
Disruption of topoisomerase II perturbs pairing in Drosophila cell culture.
Genetics 177:31-46(2007)

PubMed=23891577; DOI=10.1016/j.tiv.2013.07.007
Curtis T.M., Collins A.M., Gerlach B.D., Brennan L.M., Widder M.W., van der Schalie W.H., Vo N.T.K., Bols N.C.
Suitability of invertebrate and vertebrate cells in a portable impedance-based toxicity sensor: temperature mediated impacts on long-term survival.
Toxicol. In Vitro 27:2061-2066(2013)

PubMed=24434506; DOI=10.1016/j.ymeth.2014.01.006
Cherbas L., Gong L.
Cell lines.
Methods 68:74-81(2014)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-1963
BCRC; 60442
CCTCC; GDC0138
DSMZ; ACC-130
ECACC; 90070554 - Discontinued
KCB; KCB 200559YJ
RCB; RCB1153
Cell line databases/resources CLO; CLO_0008922
CLO; CLO_0008971
CLO; CLO_0050770
CLDB; cl4242
CLDB; cl4270
CCRID; 1101INS-PUMC000516
DSMZCellDive; ACC-130
FlyBase_Cell_line; FBtc0000999
FlyBase_Cell_line; FBtc9000001
FlyBase_Cell_line; FBtc9000006
FlyBase_Cell_line; FBtc9000008
FlyBase_Cell_line; FBtc9000009
FlyBase_Cell_line; FBtc9000011
FlyBase_Cell_line; FBtc9000068
Lonza; 957
TOKU-E; 4025
Anatomy/cell type resources BTO; BTO:0001677
Chemistry resources ChEMBL-Cells; CHEMBL3308861
PubChem_Cell_line; CVCL_Z232
Encyclopedic resources Wikidata; Q7431879
Experimental variables resources EFO; EFO_0001233
Proteomic databases PRIDE; PXD001455
Entry history
Entry creation 10-Apr-2015
Last entry update 29-Jun-2023
Version number 25